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Goat Anti-Mouse IgE-HRP抗體 | SouthernBiotech 貨號1110-05

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Goat Anti-Mouse IgE-HRP操作手冊

特點

Goat Anti-Mouse IgE-HRP抗體 (HRP標誌) | 貨號1110-05

細胞免疫專家極力推薦SouthernBiotech高品質二級抗體

美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全,包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質(FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)各種螢光標誌、另具酵素(HRP、AP)、或未標誌及Low Endotoxin/Azide Free(LE/AF)等標定。

Specificity Reacts with the heavy chain of mouse IgE
Source Pooled antisera from goats hyperimmunized with mouse IgE
Cross Adsorption Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgE covalently linked to agarose
Isotype Goat IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation Supplied in a stock solution in 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications

Quality tested applications for relevant formats include –

ELISA 1-17
FLISA

Other referenced applications for relevant formats include –

Flow Cytometry 18
Immunohistochemistry 19
Immunoblotting 20,21
Immunoprecipitation 22

Recommended Dilutions Please refer to product specific Technical Bulletin
 Cat. No. Product Description Size
1110-01  Goat Anti-Mouse IgE-UNLB 1.0 mg
1110-02  Goat Anti-Mouse IgE-FITC 1.0 mg
1110-04  Goat Anti-Mouse IgE-AP 1.0 mL
1110-05  Goat Anti-Mouse IgE-HRP 1.0 mL
1110-08  Goat Anti-Mouse IgE-BIOT 1.0 mg
1110-09  Goat Anti-Mouse IgE-PE 0.5 mg

Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |

References

  1. Costa-Pinto FA, Basso AS, Britto LR, Malucelli BE, Russo M. Avoidance behavior and neural correlates of allergen exposure in a murine model of asthma. Brain Behav Immun. 2005;19:52-60. (ELISA)
  2. Andrade MC, Menezes JS, Cassali GD, Martins-Filho OA, Cara DC, Faria AM. Alcohol-induced gastritis prevents oral tolerance induction in mice. Clin Exp Immunol. 2006;146:312-22. (ELISA)
  3. Prescott VE, Forbes E, Foster PS, Matthaei K, Hogan SP. Mechanistic analysis of experimental food allergen-induced cutaneous reactions. J Leukoc Biol. 2006;80:258-66. (ELISA)
  4. Arnaboldi PM, Roth-Walter F, Mayer L. Suppression of Th1 and Th17, but not Th2, responses in a CD8+ T cell mediated model of oral tolerance. Mucosal Immunol. 2009;2:427-38. (ELISA)
  5. Paveglio S, Puddington L, Rafti E, Matson AP. FcRn-mediated intestinal absorption of IgG anti-IgE/IgE immune complexes in mice. Clin Exp Immunol. 2012;42:1791-800. (ELISA)
  6. Kawabe Y, Hayashida Y, Numata K, Harada S, Hayashida Y, Ito A, et al. Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens. PLoS One. 2012;7(10):e48512. (ELISA)
  7. Kin NW, Stefanov EK, Dizon BL, Kearney JF. Antibodies generated against conserved antigens expressed by bacteria and allergen-bearing fungi suppress airway disease. J Immunol. 2012;189:2246-56. (ELISA)
  8. Lochmatter C, Schneider CL, Ingram K, Keiser J, Schifferli JA. Schistosoma mansoni tetraspanning orphan receptor (SmTOR): a new vaccine candidate against schistosomiasis. Clin Exp Immunol. 2012;170:342-57. (ELISA)
  9. Durham CG, Schwiebert LM, Lorenz RG. Use of the cockroach antigen model of acute asthma to determine the immunomodulatory role of early exposure to gastrointestinal infection. Methods Mol Biol. 2013;1032:271-86. (ELISA)
  10. Vukcevic M, Zorzato F, Keck S, Tsakiris DA, Keiser J, Maizels RM, et al. Gain of function in the immune system caused by a ryanodine receptor 1 mutation. J Cell Sci. 2013;126:3485-92. (ELISA)
  11. Brachs S, Turqueti-Neves A, Stein M, Reimer D, Brachvogel B, Bösl M, et al. Swiprosin-1/EFhd2 limits germinal center responses and humoral type 2 immunity. Eur J Immunol. 2014;44:3206-19. (ELISA)
  12. Zhou W, Goleniewska K, Zhang J, Dulek DE, Toki S, Lotz MT, et al. Cyclooxygenase inhibition abrogates aeroallergen-induced immune tolerance by suppressing prostaglandin I2 receptor signaling. J Allergy Clin Immunol. 2014;134:698-705. (ELISA)
  13. Joshi VB, Adamcakova-Dodd A, Jing X, Wongrakpanich A, Gibson-Corley KN, Thorne PS, et al. Development of a poly (lactic-co-glycolic acid) particle vaccine to protect against house dust mite induced allergy. AAPS J. 2014;16:975-85. (ELISA)
  14. Sun R, Tang X, Yao H, Hong S, Yang Y, Kou W, et al. Establishment of a new animal model of allergic rhinitis with biphasic sneezing by intranasal sensitization with Staphylococcal enterotoxin B. Exp Ther Med. 2015;10:407-12. (ELISA)
  15. Bracken SJ, Adami AJ, Szczepanek SM, Ehsan M, Natarajan P, Guernsey LA, et al. Long-term exposure to house dust mite leads to the suppression of allergic airway disease despite persistent lung inflammation. Int Arch Allergy Immunol. 2015;166:243-58. (ELISA)
  16. Patel PS, Kearney JF. Neonatal exposure to pneumococcal phosphorylcholine modulates the development of house dust mite allergy during adult life. J Immunol. 2015;194:5838-50. (ELISA)
  17. Chen X, Lin J, Pan T, Cao M, Shi C, Cai Q, et al. Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay. Fish Sci. 2015;81:551-8. (ELISA)
  18. Vogel L, Lüttkopf D, Hatahet L, Haustein D, Vieths S. Development of a functional in vitro assay as a novel tool for the standardization of allergen extracts in the human system. Allergy. 2005;60:1021-8. (FC)
  19. Samarasinghe AE, Hoselton SA, Schuh JM. The absence of VPAC2 leads to aberrant antibody production in Aspergillus fumigatus sensitized and challenged mice. Peptides. 2011;32:131-7. (IHC)
  20. Burns TA, Westerman T, Nuovo GJ, Watts MR, Pettigrew A, Yin C, et al. Role of oxidative tissue injury in the pathophysiology of experimentally induced equine laminitis: a comparison of 2 models. J Vet Intern Med. 2011;25:540-8. (WB)
  21. Nieuwenhuizen NE, Meter JM, Horsnell WG, Hoving JC, Fick L, Sharp MF, et al. A cross-reactive monoclonal antibody to nematode haemoglobin enhances protective immune responses to Nippostrongylus brasiliensis. PLoS Negl Trop Dis. 2013;7(8):e2395. (WB)
  22. Hazenbos WL, Wu P, Eastham-Anderson J, Kinoshita T, Brown EJ. Impaired FcεRI stability, signaling, and effector functions in murine mast cells lacking glycosylphosphatidylinositol-anchored proteins. Blood. 2011;118:4377-83. (IP)

 

 

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