D Tag Antibody 單株抗體 (1E6) | ABMGOOD貨號G191
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Anti- D Tag Antibody 單株抗體 ( 1E6) | 貨號G191
Anti- D Tag Antibody (Mouse Monoclonal)
重組技術的一個重要特徵: 將重組蛋白與蛋白質/肽標籤融合,在蛋白質上的標籤蛋白,可以幫助融合重組蛋白的溶解、細胞定位、或通過使用標籤特異性抗體Tag antibody大大簡化純化之過程。 通常使用的表位標籤包括His tag(6-組氨酸),HA tag(血凝素),cMYC tag,GST tag(穀胱甘肽-S-轉移酶)和FLAG tag(DYKDDDDK)。 abm提供了廣泛的anti- tag抗標籤抗體,每種標籤抗體對相關表位標籤表現出高度的特異性。單株Tag抗體和多株tag特異性抗體,均可用於各種應用,如Western Blotting,ELISA,IP免疫沉澱和IF免疫螢光實驗。
抗體特異性: 抗-D標籤抗體識別出轉染的哺乳動物細胞中含有與靶向蛋白質的氨基或羧基末端融合的D表位標籤的過表達蛋白質。免疫原為synthetic peptide (DYKDDDDK) coupled to KLH。D抗體辨視D tag融合蛋白。
Immunogen | A synthetic peptide (DYKDDDDK) coupled to KLH. |
Species Reactivity | other |
Specificity | Anti-D tag Antibody recognises over-expressed proteins containing D epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells. |
Tested Applications | WB, IP, Other |
Application Notes | Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows: Western Blot at dilutions of 1:1000. Immunoprecipitation amd immunostaining at dilutions of 1:1000. |
Raised In | mouse |
Clonality | 1E6 |
Isotype | IgG2b with lambda light chain |
Storage Buffer | PBS, pH 7.4 with 0.05% sodium azide. |
Formulation | Liquid |
Concentration | 100μg / 200μl |
His Tag單株抗體 | HA Tag 單株抗體 | GFP Tag 單株抗體 | D Tag 單株抗體 |GST Tag 單株抗體 | Myc Tag 單株抗體 | V5 Tag 單株抗體