總膠原蛋白測定試劑盒（無高氯酸） | Total Collagen Assay Kit (Perchlorate-Free)貨號K406-100 (ABCAM, ab222942)
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總膠原蛋白測定試劑盒（無高氯酸） | 貨號K406-100 (ABCAM, ab222942)
Total Collagen Assay Kit (Perchlorate-Free)
Estimation of total collagen content in rat tissues. Rat leg muscle, spleen, heart and lung samples were prepared followind the sample preparation procedure described in the protocol (homegenized in ddH2O, hydrolyzed with 10 N NaOH for 1 hour at 120°C and neutralized with 10 N HCl). For each sample, 10 μL of the final neutralized hydrolysate was assayed. Total Collagen levels (calculated as μg collagen/mg wet tissue) for the samples were: 13.80 +- 0.73 ug/mg for muscle, 3.77 +- 0.54 ug/mg for spleen, 4.55 +- 0.81 ug/mg for heart and 12.51 +- 1.27 ug/mg for lung. Data are mean ± SEM of 3-4 replicates.
產品描述：膠原蛋白是哺乳動物中最豐富的蛋白質，佔身體蛋白質含量的近30％。膠原蛋白是在皮膚，肌腱，骨，軟骨，韌帶和肌肉以及其他組織中發現的細胞外支架蛋白。有超過二十種不同的特徵類型的膠原蛋白，但I型，II型和III型的纖維類占哺乳動物總膠原蛋白的90％以上。膠原具有三重螺旋結構，由具有重複Gly-X-Hyp三肽序列的三個“前原膠原”鏈組成，其中X是任何氨基酸，Hyp是羥脯氨酸，由脯氨酰脯氨酸形成的翻譯後修飾的氨基酸-4-羥化酶。在動物中，羥脯氨酸幾乎全部發現在膠原中（其含有約12-14％的羥脯氨酸），有助於其結構剛性和高拉伸強度。因此，羥脯氨酸是組織中存在的膠原的量的直接測量。許多疾病會影響膠原周轉，包括腫瘤侵襲和轉移，類風濕關節炎，心肺纖維化和肌營養不良。 BioVision的全膠原測定試劑盒II（無高氯酸鹽）是一種快速便捷的方法，用於檢測各種生物樣品中的少量膠原蛋白。該測定基於樣品的水解以產生游離的Hyp，其被氧化並反應以產生在560nm具有吸收峰的明亮著色的髮色團。該測定使用專有的酸性顯影劑溶液來精確測量水解物中的膠原蛋白，而不使用有害的高氯酸，從而避免了任何特殊處理和廢物處理方案的需要。該測定法可以以96孔的形式每孔檢測至少0.5μg的膠原蛋白，並產生幾乎與經典的高氯酸方法相同的比色結果。
• Simple, fast and convenient assay
• Can detect as low as 0.5 µg of collagen in a variety of samples
• Simple procedure; takes less than ~60 minutes
• Faster than Perchlorate-Based Assay
• PCA-free
| Cat # +Size | K406-100 |
| Size | 100 Reactions |
| Kit Summary | • Detection method- Absorbance (OD = 560 nm). • Sample Types- Mammalian tissues, protein/peptide hydrolysates, serum, Urine • Applications- Measurement of collagen in various sample types. |
| Detection Method | Detection method- Absorbance (OD = 560 nm). |
| Species Reactivity | Mammalian tissues, protein/peptide hydrolysates, serum, urine |
| Applications | Measurement of collagen in various sample types. |
| Features & Benefits | • Simple, fast and convenient assay • Can detect as low as 0.5 µg of collagen in a variety of samples • Simple procedure; takes less than ~60 minutes • Faster than Perchlorate-Based Assay • PCA-free |
| Kit Components | • Oxidation Buffer • Chloramine T Concentrate • Developer Solution • DMAB Concentrate (in DMSO) • Collagen I Standard (3 mg/ml) • Microplate Sealing Film |