pGB Caspase-3 siRNA Vector 貨號1143-5

pGB expression vector to supress Caspase 3 activity in transfected cells

產品介紹: pGB表達載體抑制轉染細胞中的半胱天冬酶3活性。pGB表達載體含有人U6 RNA聚合酶III啟動子，其引導許多細胞中短RNA轉錄本的組成型，高水平表達。 每個載體還含有新黴素/卡那黴素抗性基因，以提供細菌中的卡那黴素抗性和哺乳動物細胞中的G418抗性。 pGB向量用於克隆您自己的插入。 載體包含兩個獨特的限制性位點，BamH I和Xba I用於定向克隆。 pGB陰性對照載體含有與人，小鼠和大鼠中表達的哺乳動物基因不具有顯著同源性的插入片段，可用作pGB-Caspase siRNA載體的陰性對照。 pGB胱天蛋白酶siRNA載體含有設計用於單獨抑制每種半胱天冬酶表達的siRNA插入片段。

Highlights

• (GeneBlocker™ Caspase-3 siRNA Vector, pGB-Casp-3) • Application- The pGB siRNA vectors can be transfected into mammalian cells using Lipofectamine (Invitrogen). For transient transfection, cells can be analyzed in 24-96 hours following transfections, by Western blot analysis or other detection means. For stable transfections, cells can be selected in G418 selection medium to obtain stable cell lines with the specific gene blocked.The pGB cloning vector is designed for cloning your own siRNA insert. • Features and Positions: Human U6 Promoter: 1-256 Multiple cloning Site: 259-285 3’ Primer: 398-426 (GAAGCATTTATCAGGGTTATTGTCTCATG) SV40 Promoter: 470-808 Neomycin/Kanamycin Resistance ORF: 843-1634 5’ Primer: 2789-2813 (CGTCGATTTTTGTGATGCTCGTCAG) pUC Origin of Replication: 2222-3003

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