LuminolPen™冷光筆-HRP | Visual Protein 貨號LH03-50
LuminolPen™冷光筆-HRP | 貨號LH03-50
LuminolPen™，HRP系統是Western印跡中化學發光檢測的完美工具，世界上第一款發光發光標記筆。 只需在預先染色的標記上書寫，分子量的位置即可顯示在圖像上。 它也可以幫助您注意實驗條件或評估ECL底物的功效。可以使用數據結果在圖像上進行記錄和顯示。適用於大多數品牌的ECL底材
Figure 1. Use LuminolPen™ EZ to take notes and mark prestained protein marker on the blot
Figure 1. Labeling molecular weight and note on the membrane with LuminolPen™.
(A) and (C) are general Western blot chemiluminescence detection image; (B) and (D) are the image using LuminolPen™ to indicate the pre-stained marker.
Figure 2. Distinguish target signal and non-specific binding signal with LuminolPen™
30 μg cell lysate SP-1 and detect with anti-ADNP (mouse, 1:2,000). Secondary antibody : anti-mouse IgG-HRP (1:15,000). Membrane : Hyond™ P. Detection : Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. The molecular weight note by LuminolPen™ reveal the correct target signal (ADNP 124KDa) on X-ray film.
Figure 3. Evaluate ECL substrate efficacy by LuminolPen™.
30 μg cell lysate HepaG2 and detect with anti-AMP-active protein kinase (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP (1:10,000). Membrane: Hyond™ P. Detection: Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. The signal result present by the normal ECL substrate is stronger than using the expired ECL substrate. LuminolPen™ noted marker can be an indicator to check the result or adjust different experiments to similar experimental conditions.
Figure 2. Application of LuminolPen™ EZ on the ECL-base Western Blot experiments.
Label the positions of molecular weight marker (VC01, VisualProtein) and notes the PVDF membrane with LuminolPen™ EZ. Capture the annotated signal according standard ECL reaction by image Capture System.
Reference:
1. Tsai, Yen-Yin, et al. “Identification of the nanogold particle-induced endoplasmic reticulum stress by omic techniques and systems biology analysis.” ACS nano 5.12 (2011): 9354-9369.
2. Chen, Han-Min, Ching-Yu Lin, and Vinchi Wang. “Amyloid P component as a plasma marker for Parkinson’s disease identified by a proteomic approach.” Clinical biochemistry 44.5 (2011): 377-385.
3. Chiu, Cheng‐Di, et al. “Investigation of the effect of hyperglycemia on intracerebral hemorrhage by proteomic approaches.” Proteomics 12.1 (2012): 113-123.
4. Pei-Jung Katy Chung, et al. “microRNA-205 targets tight junction-related proteins during urothelial cellular differentiation.” Molecular & Cellular Proteomics (2014) 13, 9: 2321-2336
| Cat. No. | Product Name | Description |
|
LH03-50 |
LuminolPen™, HRP System |
About 1,000 membranes drawing
|
|
LH03-10 |
LuminolPen™, HRP System |
About 100 membranes drawing
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| BR01-500 | Bradford 蛋白質定量試劑 | Dual-Range Bradford Reagent (1X), 500 ml , | |
| BR05-500 | 5X Bradford 蛋白質定量試劑 | Dual-Range Bradford Reagent (5X), 500ml | |
| BR05-500-K | 5X Bradford 蛋白質定量試劑組 |
Dual-Range Bradford Protein Assay Kit, (500 ml +10* 2mg/ml BSA standard) |
|
| BP01-1L | 西方墨點法遮蓋液 | BlockPRO Blocking Buffer, 500mlx2 | |
| BF01-1L | 西方墨點法遮蓋液-Protein Free | BlockPRO Protein-Free Blocking Buffer, 500mlx2 | |
| BF20-50P | 西方墨點法遮蓋液-Protein Free |
BlockPRO Protein-Free Blocking Buffer, 20X, 20包，每包2.5 mL， 使用前加入500mL ddH2O 回溶成 1X |
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| 西方墨點法遮蓋液-Protein Free, 20X | |||
| BM01-500 | 西方墨點法遮蓋液-1分鐘配方 | BlockPRO 1 Min Protein-Free Blocking Buffer, 500ml | |
| SP01-500 | 抗體去除試劑 | StripPRO 1 Min Stripping Buffer, 500ml | |
| LF01-500 | 冷光試劑組- Prime | LumiFlash Prime Chemiluminescent Substrate,( 250mL solnA + 250mL soln B) | |
| LF08-500 | 冷光試劑組- Ultima | LumiFlash Ultima Chemiluminescent Substrate, (250mL solnA + 250mL soln B) | |
| LF16-500 | 冷光試劑組- Infinity | LumiFlash Infinity Chemiluminescent Substrate, (250mL solnA + 250mL soln B) | |
| VC01-250 | 預染蛋白質標準液 | VisColorTM Pre-Stained Protein Marker, 250ul x 1 | |
| VC01-500 | 預染蛋白質標準液 | VisColorTM Pre-Stained Protein Marker, 250ul x 2 | |
| VC03-250 | 全範圍預染蛋白質標準液 |
VisColorTM Full-Range Pre-Stained Protein Marker, 250ul x 1
|
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| VC03-500 | 全範圍預染蛋白質標準液 |
VisColorTM Full-Range Pre-Stained Protein Marker, 250ul x 2
|
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| LH03-10 | 冷光筆 |
LuminolPen HRP system, (約 150 張 Western blot’s membranes 使用) |
|
| LH03-50 | 冷光筆 |
LuminolPen HRP system, (約1000 張 Western blot’s membranes 使用) |
|
| LH05-10 | 冷光筆 EZ版 | LuminolPen EZ HRP System, (約 150 張 Western blot’s membranes 使用) | |
| LH05-50 | 冷光筆 EZ版 | LuminolPen EZ HRP System, (約 1000 張 Western blot’s membranes 使用) | |
| RB10-1L | SDS-PAGE 電泳緩衝液 | SDS-PAGE Running Buffer , 10X (Tris-Glycine, pH 8.3), 500 ml *2 | |
| RB500P | SDS-PAGE 電泳緩衝液濃縮包 |
SDS-PAGE Running Buffer , 10X (Tris-Glycine, pH 8.3), (20包，每 包須於使用前加入500mL ddH2O 回溶成 1X) |
|
| WTB10-1L | Western Blot Transfer Buffer | Western Blot Transfer Buffer, 10X (Tris-Glycine, pH 8.3), 500 ml *2 西方墨點法轉印緩衝液 | |
| WTB500P | 西方墨點法轉印緩衝液濃縮包 |
Western Blot Transfer Buffer,(20包，每包須於使用前加入500mL ddH2O 回溶成 1X) |
|
| TBS10-1L | TBS緩衝液 | TBS Buffer, 10X, 500 mL*2 | |
| TBS200P | TBS 緩衝液濃縮包 |
TBS Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) |
|
| TBST10-1L | TBST 緩衝液 | TBS Tween-20 Buffer, 10X, 500 mL*2 | |
| TBST200P | TBST 緩衝液濃縮包 |
TBS Tween-20 Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) |
|
| PBS10-1L | PBS 緩衝液 | PBS Buffer, 10X , 500 mL*2 | |
| PBS200P | PBS 緩衝液 | PBS Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) | |
| PBST10-1L | PBST 緩衝液 | PBS Tween-20 Buffer, 10X, 500 mL*2 | |
| PBST200P | PBST 緩衝液 |
PBS Tween-20 Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) |
|
| SBN06-15 | 6x Laemmli SDS Sample Buffer, non-reducing | 6x Laemmli SDS Sample Buffer, non-reducing, 15ml | |
| SBR06-15 | 6x Laemmli SDS Sample Buffer, reducing | 6x Laemmli SDS Sample Buffer, reducing, 15ml | |
| VP01-125 | 全蛋白質負染色試劑組 |
VisPRO 5 Minutes Protein Stain Kit, (125mL soln 1 + 125mL soln2) |
|
| VP01-500 | 全蛋白質負染色試劑組 |
VisPRO 5 Minutes Protein Stain Kit, (500mL soln 1 + 500mL soln 2) |
|
| VP05-125 | 全蛋白質負染色試劑組- 五倍濃縮液 |
5x VisPRO 5 Minutes Protein Stain Kit, (125mL soln 1 + 125mL soln 2)
|
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| VP05-500 | 全蛋白質負染色試劑組- 五倍濃縮液 | 5x VisPRO 5 Minutes Protein Stain Kit, (250mL soln 1 + 250mL soln 2) | |
|
PP03-2C
|
磷酸化蛋質萃取試劑組 | PhosPRO Phosphoprotein Enrichment Kit, (2 pre-packed column + 2mL Lysis buffer+250mL System buffer) +(100mL Elution buffer+100 mL Stringent buffer stock) | |
| PP03-6C | 磷酸化蛋質萃取試劑組 |
PhosPRO Phosphoprotein Enrichment Kit, (6 pre-packed column + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock ) |
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PP03-5E
|
磷酸化蛋質萃取試劑組 |
PhosPRO Phosphoprotein Enrichment Kit, (3 mL Resin + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock) |
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PP05-2C
|
PhosPRO Phosphoprotein Purification Kit, (2 pre-packed column + 2mL Lysis buffer+250mL System buffer) +(100mL Elution buffer+100 mL Stringent buffer stock) | ||
|
PP05-6C
|
磷酸化蛋白質純化試劑組 | PhosPRO Phosphoprotein Purification Kit, (6 pre-packed column + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock) 磷酸化蛋白質純化試劑組 | |
|
PP05-5E
|
磷酸化蛋白質純化試劑組 | PhosPRO Phosphoprotein Purification Kit, (3 mL Resin + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock ) | |
| EP05-30 | 全蛋白質萃取試劑 | ExtractPRO Protein Extraction Reagent | |
| FP01-5 | 胞器蛋白質分離試劑組 | FracPRO Protein Fractionation Kit, (10mL Soln1+ 10mL Soln2 +7.5mL Soln3+1.5mL Soln4) | |
| FP01-50 | 胞器蛋白質分離試劑組 | FracPRO Protein Fractionation Kit, (100mL Soln1 + 100mL Soln2+ 75mL Soln3 +15mL Soln4) | |
| HB01-1L | 促融合瘤細胞生長因子 | HybriMore, Hybridoma cloning factor, for 1L medium dilution | |
| IF01-4N | 快速免疫佐劑 | ImmunoFast Adjuvant, for 4 injections to mouse | |
| DG03-1P | DIGE蛋白質標記試劑組 | DIGE-Kit, (Cy2 400pmole + Cy3 400pmole + Cy5 400pmole+ 20ml DMF) | |
| GLP01 | 負染膠片光箱 | Gel Lighting Plate 21 x 29.7 cm(A4) | |
| BLP01 | 正染膠片光箱 | Band Lighting Plate 21 x 29.7 cm(A4) | |
| BSB01 | 黑染盒 | Black Staining Box 13 x 13 x 4 cm (For mini gel) | |
| BSB02 | 黑染盒 | Black Staining Box 17.5 x 17.5 x 4.5 cm (For regular gel) | |
| BSB03 | 黑染盒 | Black Staining Box 20 x 24 x 5 cm | |
| BSB04 | 黑染盒 | Black Staining Box 27 x 27 x 5 cm |