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氧化鐵磁性顆粒抗體標誌套組 | 0.5μm Magnetic Bead Conjugation Kit 貨號 1300-0010 (Abcam, ab269890)

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氧化鐵磁性顆粒抗體標誌套組操作手冊

特點

Magnetic Bead Conjugation Kit

Magnetic Bead氧化鐵磁性顆粒抗體標誌套組 |貨號 1300-0010  產品規格: 10個反應 ( 10 mini kit)  (Abcam, ab269890)

產品敍述:  INNOVA提供磁性顆粒共價抗體標誌套組; 特殊處理磁性顆粒,不需再廣泛優化共軛之反應。一個步驟即可將抗體標誌於磁性顆粒上。0.5μm特殊處理氧化鐵磁性顆粒,提供高表面積和快速分離之間的平衡。磁性顆粒的結合能力:≥70µg of Goat anti-rabbit antibody/mg of beads高於市場上的其他磁性顆粒之兩倍。提供更少的顆粒,即能達到所需的靈敏度,從而將非特異性結合的風險降至最低。

Data

Figure 1. Rabbit IgG immunoprecipitation and detection by Western blot. 25 μg and 5 μg Goat anti-rabbit antibody were added to each of two Mini vials of magnetic particles, and to 25 μl of 1% magnetic beads from a leading competitor, and processed as per the respective protocols. The conjugates were used to immunoprecipitate rabbit IgG from rabbit serum. The eluted samples were run by SDS-PAGE and analysed by Western blot against rabbit IgG. Innova’s Magnetic Conjugation Kit has a higher binding capacity and coupling efficiency than the competitor products, and allows you to detect the antigen of interest with fewer reagents.

Figure 2. Magnetic Conjugation Kit binding capacity. Goat anti-rabbit antibody was conjugated to 25 μl of 1% magnetic particles from Innova’s conjugation kit (1h procedure), and to magnetic particles from a leading competitor (4h procedure) as per the respective protocols. The quantity of IgG added to the magnetic particles, and the amount of unbound IgG at the end of the conjugation, were qualified by Bradford protein assay. Innova’s Magnetic Conjugation Kit demonstrates a considerably higher binding capacity.

Features Benefits
Quick and easy to use Save time, no special knowledge required
High binding capacity Achieve high sensitivity with less material
High coupling efficiency No antibody loss during conjugation
Covalent bond Highly stable conjugates
Freeze dried Ships at ambient temperature, long shelf-life
Fully scalable Easy transfer from R&D to manufacturing
No extensive optimization required Save precious antibody / protein
Can be used in a wide range of applications Flexible

熱門商品 400nm乳膠珠抗體標誌套組 (紅色) | 400nm乳膠珠抗體標誌套組 (藍色) | 400nm乳膠珠抗體標誌套組 (黑色)相關商品 Streptavidin-gold奈米金粒子(40nm) | Streptavidin-gold奈米金粒子(20nm) | Biotin-gold奈米金粒子(40nm) | 10-80nm奈米金抗體標誌套組 | 奈米金溶液

 

 

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