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【SOD超氧化物歧化酶檢測】Superoxide Dismutase (SOD) 活性分析Abcam (Biovision)K335-100

Superoxide Dismutase (SOD) 活性分析套組 | 貨號K335-100 

biovision商品插圖

原理介紹Biovision提供一系列氧化相關分析套組;其中SOD活性分析套組,可選擇吸收光Absorbance (450 nm)偵測;可用於細胞組織supernatants、尿液、血漿、或血清之液體型式的樣品來源。操作時間大約30分鐘。超氧化物歧化酶(SOD)是最重要的抗氧化酶 (antioxidative enzymes)之一。 它催化超氧陰離子( superoxide anion)分解為過氧化氫( hydrogen peroxide )和分子氧(molecular oxygen)。SOD測定試劑盒利用WST-1,其在用超氧化物陰離子( superoxide anion)還原時產生水溶性染料。 超氧陰離子還原的速率與黃嘌呤氧化酶( xanthine oxidase, XO)活性線性相關,並且被SOD抑制。 SOD的抑制活性可以通過呈色法測定。Superoxide dismutase (SOD) is one of the most important . It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen. The sensitive SOD assay kit utilizes WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD (below). Therefore, the inhibition activity of SOD can be determined by a colorimetric method.

相關產品 觸酶Catalase活性分析 | 骨髓過氧化酶(Myeloperoxidase) 呈色分析 |Hydrogen Peroxide分析套組  | 總抗氧化物呈色分析套組

Superoxide Dismutase (SOD)活性分析套組 | K335-100內容物
• WST Solution
• SOD Enzyme Solution
• SOD Assay Buffer
• SOD Dilution Buffer

Cat # +Size K335-100
Size 100 assays
Detection Method Absorbance (450 nm)
Applications Kit contains the necessary reagents for convenient measurement of activity of Superoxide Dismutase (SOD) by colorimetric method.
Features & Benefits • Simple one-step procedure; takes around than 30 minutes
• Fast and convenient
Kit Components • WST Solution
• SOD Enzyme Solution
• SOD Assay Buffer
• SOD Dilution Buffer
K335
Madhusmita Dhupal, et al., ( 2018) Immunotoxicity of titanium dioxide nanoparticles via simultaneous induction of apoptosis and multiple toll-like receptors signaling through ROS-dependent SAPK/JNK and p38 MAPK activation, International journal of Nanomedicine, Oct. 2018, 30425486
Jingsi Zhang, et al., (2018) Jia-Jian-Di-Huang-Yin-Zi decoction exerts neuroprotective effects on dopaminergic neurons and their microenvironment, Scientific Reports, Jun. 2018, 29959371
Lin, CH. et al., (2017) Activation of AMPK is neuroprotective in the oxidative stress by advanced glycosylation end products in human neural stem cells, Experimental Cell Research, Oct.2017, 359( 2): 367-373
Baysal, M et al., (2017) Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress, PLoS One, 2017, 12(4): e0175990
Ga, Yoon Na et al. (2017) The relationship between circulating neutrophil gelatinase-associated lipocalin and early alteration of metabolic parameters is associated with dietary saturated fat intake in non-diabetic Korean women, Endocr J. 2017 Mar 31;64(3):303-314.
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