【Minute™蛋白萃取】哺乳動物、海洋動物、昆蟲、植物和細菌胞器蛋白萃取
Minute™蛋白萃取套組,使用創新『Column Filter』技術,效果更佳,完成只需 一 分 鐘
蛋白萃取只需要vortex、transfer、spin
- 簡單四步驟完成,相較同類型產品節省6-12個步驟,降低加藥產生的錯誤。
- 無須長時間等待,節省至少25分鐘!
- 免超音波震盪、避免蛋白加熱與變性。
- 免除重複性的wash步驟。
Minute™蛋白萃取套組,使用創新『Column Filter』技術,效果更佳,完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒;適用於多種物種(包括哺乳動物、海洋動物、昆蟲、植物和細菌)快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同,優化的緩衝液系統,結合最新的專有濾筒技術(spin column);快速提高蛋白萃取的效能及方便性;用於總蛋白提取,細胞分離和膜蛋白分離,只需簡單四步驟即可完成蛋白質萃取。無須長時間等待、免除重複性的wash步驟。
Figure 1. A. SDS-PAGE profiles of total cell lysate (TC) and isolated plasma membrane proteins (PM) from human and rat cultured cells. Lane 1, Human lung cancer cell A549 total cell lysate; Lane 2, Isolated PM of A549; Lane 3, PM of rat REL-6TN cells; Lane 4, Total cell lysate of rat REL-6TN cells. B. Western blottings : Proteins in A were transferred to nitrocellulose membrane and probed with anti-Na/K ATPase alpha1, a plasma membrane marker (Upstate, Clone 464.6)) and anti-lamin B1, a nuclear envelope marker (ab16048, abcam Cambridge, MA). The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD). C. Densitometry measurement of Na/K ATPase alpha1 signals in B (TC vs. PM). Total cell lysates were extracted by Minute Denaturing Total Protein Extraction Kit (SD-001, Invent Biotechnologies, Eden Prairie, MN).
Figure 2 A. SDS-PAGE profiles of isolated total membrane proteins from mouse tissues (T=Total Cell lysate, C=Cytosol Fraction, M= Total Membrane Fraction) B. Proteins shown in A were transferred to a nitrocellulose membrane and probed with rabbit-anti mouse pan-cadherin (ab6529,abcam, Cambridge, MA), and anti-actin by Western blotting. The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD). Signals of pan-cadherin (a 130 kda plasma membrane marker) were significantly enhanced in total membrane protein fractions. Total cell lysates were extracted by MinuteTM Denaturing Total Protein Extraction Kit (SD-001, Invent Biotechnologies, Eden Prairie, MN).
Figure 3. A. SDS-PAGE profiles of total membrane protein fraction (TM) and isolated plasma membrane protein fraction from mouse tissues.
B. Western blotting of proteins in A were transferred to nitrocellulose membrane and probed with rabbit anti-cadherin (abcam, Cambridge, MA). The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD). C. Densitometry measurement of cadherin signals in B (TM vs. PM).
Item | Amount | Part # |
Buffer A | 25 ml | SM-005-A |
Buffer B | 10 ml | SM-005-B |
Protein Extraction Filter Cartridges | 50 | P-001 |
Collection tubes with cap | 50 | P-002 |
Plastic rods | 4 | P-003 |
Tissue dissociation beads | 10 g | P-004 |
Minute™ 膜蛋白萃取套組 | Plasma Membrane Protein Isolation Kit 貨號SM-005
Minute™質膜(PM)蛋白分離試劑盒 (plasma membrane (PM) protein isolation kit);無洗滌劑和EDTA)的PM蛋白分離試劑盒;結合獨特的專利技術機制,細胞/組織只需通過Filter即完成質膜蛋白的萃取。在該過程中,細胞膜破裂不必使用傳統的均質化或超音波處理、不需要超速離心。處理量:1×106-50×106 cells/sample。時間:<45 min。Yield:1-3 mg/ml
(Native) Minute™細胞質和核提取試劑盒| Minute™ Cytoplasmic & Nuclear Extraction Kits貨號SC-003
Minute™細胞質和核提取試劑盒,15分完成萃取,可萃取1-3mg/ml蛋白質含量。由優化的細胞質提取緩衝液、核蛋白提取緩衝液和2.0ml收集管的蛋白質提取濾筒組成。該試劑盒設計用於從培養的哺乳動物細胞或組織中快速分離天然細胞質和核蛋白。由於使用蛋白質提取濾筒,可以在不到15分鐘內完成細胞質和核蛋白的分離。可應用於乳動物細胞 (mammalian cells)和植物( plant),細菌(bacteria),酵母( yeast ) 和真菌的原生質體(protoplasts)。
Minute™ 植物蛋白提取試劑盒 | Detergent-Free Plant Protein Extraction貨號SN-010
Invent Biotechnologies’Minute™植物蛋白提取試劑盒由優化的蛋白質提取緩衝液 ( detergent-free) 和蛋白質提取濾芯組成,採用2.0 ml採集管。 該試劑盒旨在從植物組織中快速提取無去污劑 ( detergent-free)的總蛋白質。 蛋白質提取緩衝液不含任何去污劑和有機溶劑。 由於使用了蛋白質提取濾芯,提取體積可以低至50μl和500μl。 無菌的總蛋白質可以在不到8分鐘內以非常小的植物樣品(葉,種子,軟莖等)從高產量(1-6毫克/毫升)中提取。
Minute™蛋白萃取套組,使用創新『Column Filter』技術,效果更佳,完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。Minute ™一系列蛋白質提取試劑盒;適用於多種物種(包括哺乳動物、海洋動物、昆蟲、植物和細菌)快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同,優化的緩衝液系統,結合最新的專有濾筒技術(spin column);快速提高蛋白萃取的效能及方便性;用於總蛋白提取,細胞分離和膜蛋白分離,只需簡單四步驟即可完成蛋白質萃取。無須長時間等待、免除重複性的wash步驟。
Item | Amount | Part # |
DF-lysis buffer (with 5 g beads) | 25 ml | SN-010-L |
Protein Extraction Filter Cartridges | 50 | P-001 |
Collection Tubes with Cap | 50 | P-002 |
plastic rods |
真菌蛋白(細胞壁)提取試劑盒(detergent-free) | Invent貨號YT-015
Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps)
適用於酵母(yeast),絲狀真菌(filamentous fungus),革蘭氏陽性(gram-positive)和革蘭氏陰性細菌(negative bacteria),昆蟲卵(insect eggs),微藻類(microalgae)等蛋白質萃取
產品介紹: 含有細胞壁的微生物(cell-wall-containing microbes) 中提取蛋白質是一種常見程序。YT-015提供了一種無需儀器,快速而溫和的方式,可從具有厚而堅固的細胞壁的微生物 (microbes) 中提取蛋白質。這些微生物包括酵母(yeast),絲狀真菌(filamentous fungus),革蘭氏陽性(gram-positive)和革蘭氏陰性細菌(negative bacteria),昆蟲卵(insect eggs),微藻類(microalgae)等。該試劑盒包含優化的變性(denaturing protein extraction buffers 貨號) 和天然蛋白質提取緩衝液 (native protein extraction buffers)供您選擇。與許多其他苛刻條件下的方法不同(例如8 M尿素),使用酵母進行儀器進行玻璃珠裂解並用鹼萃取將樣品煮沸等方法用於酵母蛋白質萃取。通常使用這些方法優先提取某些分子量範圍內的蛋白質。該試劑盒具有單管操作規程,可以無偏差地提取所有種類的酵母蛋白。該試劑盒適用於對數期(log-phase)和固定期(stationary-phase)之微生物。整個過程不到10分鐘即可完成,蛋白質產量在2-5 mg / ml的範圍內。提供的材料足以進行50次反應。
套組內容物Kit Components | Amount | Part # |
Cytoplasmic Extraction Buffer | 25 ml | SC-003-L |
Nuclear Extraction Buffer | 25 ml | SC-003-N |
Protein Extraction Filter Cartridges | 50 | P-001 |
Collection Tubes with Cap | 50 | P-002 |
Sub-Cellular Structure Fractionation/Isolation | |||
Cat # | Description | Size | |
SC-003 | Cytoplasmic & Nuclear Extraction Kits | 50 Preps | |
SM-005 | Plasma Membrane Protein Isolation Kit | 50 preps | |
NE-013 | Nuclear Envelope Protein Extraction Kit | 50 preps | |
MP-007 | Mitochondria Isolation Kit For Mammalian Cells And Tissues | 50 preps | |
AF-023 | Adipose Tissue Fractionation Kit | 20 preps | |
NI-024 | Detergent-free nuclei isolation kit | 20 preps | |
YM-017 | Yeast Mitochondria Enrichment Kit | 50 preps | |
MM-018 | Plant Microsomal Membrane Extraction Kit | 50 preps | |
CP-011 | Chloroplast Isolation Kit | 50 preps |
Specialty Kits | |||
Cat # | Description | Size | |
PN-019 | Protein/Nucleic Acid Extraction Kit from Gels | 50 preps | |
SC-012 | Single Cell Isolation Kit | 50 preps | |
HP-014 | Histone/DNA Binding Protein Extraction Kit | 50 preps |
Protein Analysis Reagents | |||
Cat # | Description | Size | |
WA-001 | HRP Substrate Kit | 30 ml/30 ml | |
WA-002 | Antibody Enhancer | 30 ml | |
WA-003 | Western Blot Stripping Solution | 250 ml | |
WA-004 | Ponceau S Stain | 250 ml | |
WA-005 | Peroxidase Suppressor | 30 ml | |
WA-006 | High Efficiency Protein Precipitation kit | 30 ml/30 ml | |
WA-007 | RBC Lysis Buffer |