CCK-8細胞增殖和細胞毒性試劑盒 | 貨號 KTA1020   吸收光450 nm    CCK8操作手冊 

Abbkine Cell Counting Kit-8（CCK-8）設計用於檢測細胞增殖和細胞毒性。

細胞計數試劑盒-8（CCK-8）使用高度水溶性的四唑鹽WST-8：[2-（2-甲氧基-4-硝基苯基）-3-（4-硝基苯基）-5-（2,4-二磺基苯基 ）-2H-四唑鎓單鈉鹽]通過還原轉化為水溶性橙色甲dye染料。 CCK-8具有低毒性，並且允許用於測定細胞增殖和細胞毒性測定中活細胞數量的靈敏比色測定。 細胞中脫氫酶產生的甲dye染料的量與活細胞的數量成正比。Cell Counting Kit-8（CCK-8）用於檢測細胞增殖(cell proliferation)及細胞毒性(cell toxicity)，其原理基於WST-8經細胞中的脫氫酶降低，得到橙色產物（formazan）。細胞中formazan的含量與活細胞數成正比。細胞計數試劑盒8（Cell Counting Kit-8, CCK-8）通過使用高度水溶性的四唑鹽-WST-8進行非常方便的測定。[2-（2-甲氧基-4-硝基苯基）-3-（4-硝基苯基）-5- （2,4-二磺基苯基）-2H-四唑鎓鹽]在電子介體的存在下還原生成水溶性formazan dye染料。 CCK-8是非放射性的，可進行敏感的比色測定，以測定細胞增殖(cell proliferation)和細胞毒性測定中的活細胞數量。 WST-8被細胞中的脫氫酶(dehydrogenases)還原，得到橙色產物(formazan)，可溶於組織培養基。細胞中脫氫酶(dehydrogenases) 產生的formazan dye染料的量與活細胞的數量成正比。WST-8生產的產品(formazan)是水溶性的，不需要有機溶劑或同位素。而且formazan是穩定和安全的。使用CCK-8的檢測靈敏度高於使用其他四唑鹽（tetrazolium salts, 如MTT，XTT，MTS或WST-1）的檢測靈敏度。

Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8.[2-(2- methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. The product (formazan) produced by WST-8 is water soluble, no organic solvents or isotopes required. And the formazan is stable and safe. The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.

Fig. Abbkine Economical Cell Counting Kit-8 (CCK-8) is designed to detect cell proliferation and cell toxicity.

CCK-8; WST-8; CCK8; WST8

Product name	Cell Counting Kit-8 (CCK-8)
Alternative	CCK-8; WST-8; CCK8; WST8
Formulation	Liquid solution
Kit components	• CCK-8 solution
Features & Benefits	• One-bottle, ready-to-use solution. • The product (formazan) produced by WST-8 is water soluble, No organic solvents or isotopes required. And the formazan is stable and safe. • The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1. • The same cells can be used for other cell assays because of the low toxicity of CCK-8.
Product name	Cell Counting Kit-8 (CCK-8)
Alternative	CCK-8; WST-8; CCK8; WST8
Formulation	Liquid solution
Kit components	• CCK-8 solution
Features & Benefits	• One-bottle, ready-to-use solution. • The product (formazan) produced by WST-8 is water soluble, No organic solvents or isotopes required. And the formazan is stable and safe. • The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1. • The same cells can be used for other cell assays because of the low toxicity of CCK-8.
ackground	Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8.[2-(2- methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. The product (formazan) produced by WST-8 is water soluble, no organic solvents or isotopes required. And the formazan is stable and safe. The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
Alternative	CCK-8; WST-8; CCK8; WST8