抗氧化能力分析檢測Analysis and detection of antioxidant capacity

生物體內有許多抗氧化劑(antioxidants)，如SOD、CAT、GSHPx、G6PD、GR、穀胱甘肽(glutathione)等，可以清除體內各種活性氧，防止活性氧(reactive oxygen species)誘導的氧化應激(oxidative stress)的產生。一個系統中各種抗氧化大分子、抗氧化小分子和酶的總水平反映了系統的抗氧化能力(antioxidant capacity)。 雖然 ROS 誘導的氧化損傷的分析被廣泛用作分析指標，但檢測體內細胞、細胞和提取物的抗氧化能力水平也很重要。 細胞內大分子的抗氧化能力可以通過ORAC （antioxidant capacity of oxygen group，氧自由基抗氧化能力 (ORAC) 螢光檢測）、HORAC（antioxidant capacity of hydroxyl group,(HORAC) 螢光檢測）和TAC總抗氧化能力檢測（total antioxidant capacity, TAC）來衡量。

Abbkine 緊隨 Cell Biolabs 的 ORAC 和 HORAC 生化分析。檢測方法為過氧化氫自由基(hydrogen peroxide radical)通過氫原子轉移反應氧化螢光探針(oxidized fluorescence probe)。在組織細胞中，過氧化氫自由基(hydrogen peroxide free radicals)是由其他引發自由基的氧化(free radicals)產生的。在檢測過程中，待測樣品中的抗氧化劑抑製過氧化氫自由基(hydrogen peroxide free radical)對螢光探針的氧化，直至其抗氧化能力(antioxidant capacity)喪失。這樣，剩餘的過氧化氫自由基會繼續氧化破壞螢光探針的螢光效應(fluorescence effect)，直到抗氧化劑的抑制時間和自由基損傷(free radical damage)的抑制達到平衡(balanced)。因此，待測樣品的抗氧化能力(antioxidant capacity)與螢光衰減曲線(fluorescence decay curve)有關。

氧自由基吸收能力（Oxygen Radical Absorbance Capacity , ORAC）是檢測各種樣品中生物分子抗氧化能力(antioxidant Capacity)的經典方法。 ORAC法具有特異性(specificity)好、靈敏度高(high sensitivity)、測定範圍寬(wide determination range)、抗氧化活性(antioxidant activity)篩選高通量等優點。 它已越來越多地應用於天然產物抗氧化提取物。 食品和功能性食品(functional food)企業普遍採用ORAC作為功能性食品的重要評價標準。

羥基自由基吸光度(Hydroxyl Radical Absorbance Capacity , HORAC)是一種檢測各種樣品中生物分子抗氧化能力的方法，可以反映抗氧化劑轉移羥基自由基(Hydroxyl free radicals)的能力

(HORAC) 螢光檢測 | TAC總抗氧化能力檢測  | 氧自由基抗氧化能力 (ORAC) 螢光檢測

CheKine™總抗氧化劑能力分析 | CheKine™ Total Antioxidant Capacity (TAC) Assay Kit貨號KTB1500  偵測範圍: 0.15-3 mM

偵測範圍: 0.15-3 mM

Fig. Standard Curve of Total Antioxidant Capacity (TAC) Assay. The y-axis is OD593 and the x-axis is Fe2+ concentration (mM).

抗氧化劑(Antioxidants)在防止自由基和其他潛在有毒氧化物質的形成和清除方面(scavenging of free radicals )發揮著重要作用。抗氧化劑(antioxidant species)種類有三類：酵素系統穀胱甘肽還原酶(GSH reductase)、過氧化氫酶(catalase)、過氧化物酶(peroxidase)等、小分子抗壞血酸(ascorbate, uric acid)、尿酸(uric acid)、穀胱甘肽(GSH)、維生素E等及蛋白質白蛋白(albumin)、轉鐵蛋白( transferrin)等。CheKine™總抗氧化劑能力（Total Antioxidant Capacity , TAC）測定試劑盒提供了一種簡單易用的比色測定法，用於測量血清，血漿，細胞培養上清液，尿液，組織/細胞裂解液和其他生物流體中的總抗氧化劑能力。 在該測定中，Fe3 + -TPTZ被抗氧化劑還原為Fe2 + -TPTZ。 酶催化的反應產物Fe2 + -TPTZ可以在比色讀數為593 nm的條件下進行測量。偵測範圍: 0.15-3 mM。可應用於以下樣品來源: serum, plasma, cell culture supernatants, urine, tissue/ cell lysates and other biological fluids.

(文獻引用出自) The total antioxidant capacity (TAC) and hydroxyl free radical scavenging capacity assays revealed the ability to remove H₂O₂ and –OH, reflecting the antioxidant ability of the targeted reagent. The results in Figure 4A indicated that the moderate combination with peptide at 600:1 did not weaken the total antioxidant capacity of tFNA.

Kit components	• Assay Buffer (10X) • Substrate Diluent • Substrate • Reaction Buffer • ascorbic acid (positive control)
Features & Benefits	• Determination of Total Antioxidant Capacity in serum, plasma, cell/tissue samples, urine and bacteria samples. • Using a stronger antioxidant, sascorbic acid as positive control, instead of the frequently-used Trolox. • The reaction is carried out under acidic conditions, which can inhibit some endogenous interference factors.
Usage notes	• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range. • Fresh samples are necessary for good results. If not assayed immediately, samples can be stored at -80°C for one month. • Can not use EDTA as the anticoagulant for serum and plasma samples. The samples also can not contain DTT, Mercaptoethanol, Tween, Triton, NP-40.
Storage instructions	Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.