DNase I  去氧核糖核酸酶I | 2000 U (1.0 ml) 2U/ul | 貨號G028

產品介紹: DNase I，即Deoxyribonuclease I，中文名稱為去氧核糖核酸酶I，是一種可以消化單鏈或雙鏈DNA產生單去氧核苷酸或單鏈或雙鏈的寡去氧核苷酸的核酸內切酶。DNase I水解單鏈或雙鏈DNA後的產物，5’端為磷酸基團，3’端為羥基。

DNase I (2000U) | Applied Bioilogical Materials Inc.

活性定義：37℃10分鐘內，將能夠完全降解1μg pBR322質粒DNA所需的酶量定義為1個活性單位。

純度：不含其它DNA40mM Tris-HCl(pH8.0)，10mM MgSO4，1mM CaCl2，1μg of pBR322 DNA。內切酶和外切酶，不含RNA酶。

酶儲存溶液：10 mM Tris-HCl (pH 7.6), 2 mM CaCl2, and 50% (v/v) Glycerol.• Removes DNA from protein preparations and RNA samples

失活或抑制：加入EDTA至終濃度為2.5mM後，65℃加熱10分鐘可使DNase I失活。酚氯仿抽提也可以使DNase I失活。金屬離子螯合劑，達到毫摩爾/升濃度的鋅離子，0.1%的SDS，DTT、巰基乙醇等還原劑，50-100mM以上鹽濃度均對DNase I有顯著抑制作用。

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Product	Quantity	Cat No.
2-Mercaptoethanol	100 ml	CH044
2-Mercaptoethanol	200ml (2 x 100ml)	CH045
3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs	50 Tabs	CH027
3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs	100 Tabs	CH028
Actinomycin D	5.0 mg	CH001
Agarose	100 g	G060-1
Agarose	500 g	G060-2
Agarose II, Low Gelling Temperature	25 g	CH002
Agarose II, Low Gelling Temperature	100 g	CH003
Albumin, Bovine 20mg/ml solution	1.0 ml	CH006
Albumin, Bovine 20mg/ml solution	5.0 ml	CH007
Albumin, Bovine pH 5.1 +/-0.2, Salt Free Form	25 g	CH004
Albumin, Bovine pH 5.1 +/-0.2, Salt Free Form	100 g	CH005
Ammonium Persulfate (APS)	25 g	CH008
Ammonium Persulfate (APS)	100 g	CH009
Aprotinin	10 mg	CH010
Aprotinin	50 mg	CH011
B-Galactosidase	1.0 kU	CH036
B-Galactosidase	5.0 kU	CH037
BCIP, 5-Bromo-4-Chloro-3-Indolyl Phosphate	100 mg	CH012
BCIP, 5-Bromo-4-Chloro-3-Indolyl Phosphate	500 mg	CH013
BCIP, 5-Bromo-4-Chloro-3-Indolyl Phosphate Toluidine Salt	1.0 g	CH014
BCIP/NBT	100 ml	CH015
Beef Extract Powder	50 g	CH016
Beef Extract Powder	100 g	CH017
Beef Extract Powder	500 g	CH018
Bromophenol Blue	5.0 g	G114
Bromophenol Blue	25 g	CH019
Bromophenol Blue	50 g	CH020
Bromophenol Blue	100 g	CH021
Bromophenol Blue, Sodium Salt	50 g	CH022
Bromophenol Blue, Sodium Salt	100 g	CH023
Coomassie Brilliant Blue R-250	10 g	CH024
Coomassie Brilliant Blue R-250	25 g	CH025
Coomassie Brilliant Blue R-250	50 g	CH026
Cumate	500 μl	CH065
DNA Loading Dye	6X; 3 x 1.0 ml	G030
DNA Loading Green Dye	6X; 3 x 1.0 ml	G924
DNA, Fish Sperm, Sodium Salt	5.0 g	CH029
DNA, Fish Sperm, Sodium Salt	10 g	CH030
DNA, Fish Sperm, Sodium Salt	25 g	CH031
DNase I	2000 U (1.0 ml)	G028
EDTA, Disodium Salt, Dihydrate	500 g	CH032
EDTA, Disodium Salt, Dihydrate	1.0 kg	CH033
EDTA, Disodium Salt, Dihydrate	2.5 kg	CH034
Glycerol	500 ml	G115
Glycerol	100 ml	CH038
Glycogen	20 mg/ml; 1.0 ml	G024
Hepes, Sodium Salt	25 g	CH039
Hepes, Sodium Salt	100 g	CH040
Hepes, Sodium Salt	500 g	CH041
IPTG	1.0 g	G116
LB Agar	500 g	G247
LB Broth	500 g	G246
Neutral Red	5 x 1.0ml	G035
Nitroblue Tetrazolium Chloride	500 mg	CH046
Nitroblue Tetrazolium Chloride	1.0 g	CH047
Oligo(dT)18 Primer	1.0OD, 500 μl, 10 μM	G140
Pepstatin	5.0 mg	CH048
Polybrene	1.0 ml; 0.8 mg/ml	G062
Proteinase K	1.0 ml	G029
Random Primer (6N)	1.0OD, 500 μl, 10 μM	G139
RNase A	25 mg	G117
RNase A Solution	1.0 ml; 10 mg/ml	G118
RNaseOFF Ribonuclease Inhibitor	4,000 U (100 µl)	G138
Sodium Azide	250 g	CH052
Tetracycline, Hydrochloride	25 g	CH054
Tetracycline, Hydrochloride	100 g	CH055
TRIS	500 g	CH058
TRIS	1kg (2 x 500g [CH058])	CH059
TRIS	5kg (10 x 500g [CH058])	CH060
Triton X-100, T-Octylphenoxypolyethoxyethanol	1L (2 x 500ml)	CH061
Triton X-100, T-Octylphenoxypolyethoxyethanol	4.0 L	CH062
TWEEN 20, Polyoxyethylene-20-Sorbitan Monolaurate	1.0 L	CH063
TWEEN 20, Polyoxyethylene-20-Sorbitan Monolaurate	4.0 L	CH064