肝細胞生長因數(HGF)檢測試劑盒| CLIA Kit for Hepatocyte Growth Factor (HGF)
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肝細胞生長因數(HGF)檢測試劑盒(化學發光免疫分析法) |貨號SCA047Mu
CLIA Kit for Hepatocyte Growth Factor (HGF)
商品: 肝細胞生長因數(HGF)檢測試劑盒(化學發光免疫分析法) |貨號SCA047Mu，偵測的靈敏度1.37-1000pg/mL、最低靈敏度0.63pg/mL。
NEW化學發光免疫分析(CL IA ) 是用化學發光劑直接標記抗原或抗體的免疫分析方法。化學發光酵素免疫分析( chemiluminescen t enzyme immunoassay,CLIA ) ，亦屬酵素免疫分析, 只是酵素的底物是發光劑，操作步驟與ELISA分析完全相同。CLIA是將具有高靈敏度的化學發光測定技術，與高特異性的免疫反應二項技術相結合，主要用於各種抗原、半抗原、抗體、激素、酶、脂肪酸、維生素和藥物等的檢測分析方法。CLIA繼放射免疫分析、酵素免疫分析、
Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法))/CLIA (化學發光酵素免疫分析)，專門生產研究用檢測試劑的生技公司，於美國研發和生產，產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein；ELISA產品系列超過6000種以上，物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。
肝細胞生長因數(HGF)檢測試劑盒套組內容
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Substrate A | 1×10mL | Substrate B | 1×2mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
免疫分析法的概括流程
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
|肝細胞生長因數(HGF)檢測相關之抗體 | 蛋白質 | ELISA | CLIA產品
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications |
| RPA047Mu02 | Recombinant Hepatocyte Growth Factor (HGF) | SDS-PAGE; WB; ELISA; IP. |
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