液態重組蛋白A-Protein A Liquid |IgG結合位，純化單株或多株抗體
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液態重組蛋白A-Protein A (Liquid form)
產品貨號: 6500-10/6500-25/6500-100/6500-1000 |包裝10mg/25mg/100mg/1g
產品規格: Physical Form Description: 50 mg/ml in H2O、Molecular Weight: 39.21 kDa
Purity by SDS-PAGE: ≥99%、Purity by HPLC: ≥99%、Endotoxin Level: <0.1 ng/μg
應用於抗體之純化。含有IgG結合位，可和大多數免疫球蛋白之Fc或輕鏈區域結合，純化單株或多株抗體。其應用之樣品，可來自培養液culture media、血清serum、腹水ascites fluid或Hybridoma supernatants；亦可分離Immunoprecipitation實驗中抗體與抗原的複合物。
Background Information: The recombinant Protein A is a genetically engineered protein containing five Ig-binding regions of protein A and 6x His-tag on N-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
Figure: Purification of IgG using Protein A Sepharose (Cat. # 6501): (a) SEC analyses of IgG purified from rabbit serum. 1 ml rabbit serum in Protein A IgG Binding Buffer was loaded on 1 ml Protein A Sepharose & IgG was purified according to the protocol. 200 µl of starting sample (A) (serum) and purified IgG (B) were analyzed on Superdex 200 HR 10/30 column at 0.5 ml/min in 50 mM Tris, 0.25 M NaCl pH 7.5. (b) SDS-PAGE (10%) of purified IgG under reduced conditions. Lane 1: Marker, Lane 2: IgG fraction (5 µg).
Protein A／G／L重組蛋白介紹
Protein A、G和L已被廣泛地用於抗體之純化；BioVision公司提供廣泛的Agarose，Sepharose及Protein A、Protein G、Protein L、ProteinA/G、ProteinA/G/L磁珠商品。其含有IgG結合位，可和大多數免疫球蛋白之Fc或輕鏈區域結合，純化單株或多株抗體。其應用之樣品，可來自培養液culture media、血清serum、腹水ascites fluid或Hybridoma supernatants；亦可分離Immunoprecipitation實驗中抗體與抗原的複合物。
Protein A／G／L重組蛋白產品特點
- 專一性高：已排除白蛋白、細胞壁、及其他非專一蛋白結合區。
- 選擇性多：有Protein A、Protein G、Protein L、Protein A/G、Protein A/G/L等商品。依不同實驗需求提供磁珠、Agarose／Sepharose、Sepharose Column等產品
- 品質佳：SDS-PAGE和HPLC純化，純度＞98％；內毒素＜1EU/mg
- 具有高效的抗體結合能力
- 高化學和物理穩定性
- 展現高流速、親水性和高凝膠強度
Protein A、G：從E. coli純化出僅包含IgG binding domains的蛋白，可和大多數免疫球蛋白之Fc區域（如重鏈）結合。Protein L：包含Kappa light chain binding region，可以和各類Ig（IgG、IgM、IgA、IgE和IgD）的輕鏈結合。除此之外，Single chain variable fragments（ScFv）和 Fab fragments也會和Protein L結合。
不同型式之beads商品
| Catalog # | Product Name+ | Size |
| 7934-1 | Calmodulin-Sepharose Beads | 1 ml |
| 7934-5 | Calmodulin-Sepharose Beads | 5 ml |
| 7934-10 | Calmodulin-Sepharose Beads | 10 ml |
| 7933-1 | Hi-Bind™ Albumin-IgG Depletion Beads | 1 ml |
| 7933-5 | Hi-Bind™ Albumin-IgG Depletion Beads | 5 ml |
| 7933-25 | Hi-Bind™ Albumin-IgG Depletion Beads | 25 ml |
| 7931-1 | Immobilized Catalase Beads | 1 ml |
| 7931-10 | Immobilized Catalase Beads | 10 ml |
Protein A重組蛋白相關商品
Protein G重組蛋白相關商品
| Catalog # | Product Name+ | Size |
| 6513-1 | Hi-Bind™ Protein G-Agarose | 1 ml |
| 6513-5 | Hi-Bind™ Protein G-Agarose | 5 ml |
| 6513-25 | Hi-Bind™ Protein G-Agarose | 25 ml |
| 6513-100 | Hi-Bind™ Protein G-Agarose | 100 ml |
| 6510-1 | Protein G | 1 mg |
| 6510-10 | Protein G | 10 mg |
| 6510-100 | Protein G | 100 mg |
| 6510-1000 | Protein G | 1 g |
| 6510-5 | Protein G | 5 mg |
| 5510-100 | Protein G Antibody | 100 µg |
| 6522-5 | Protein G Coated 96-well Plates | 5 plates |
| 6517-1 | Protein G Magnetic Beads | 1 ml |
| 6511-1 | Protein G Sepharose | 1 ml |
| 6511-100 | Protein G Sepharose | 100 ml |
| 6511-25 | Protein G Sepharose | 25 ml |
| 6511-5 | Protein G Sepharose | 5 ml |
| 6518-1 | Protein G Sepharose Column | 1 ml |
| 6518-5 | Protein G Sepharose Column | 5 ml |
| 6512-100 | Protein G-Biotin | 100 µg |
| 6512-1000 | Protein G-Biotin | 1 mg |
Protein L重組蛋白相關商品
| Catalog # | Product Name+ | Size |
| 6530-1 | Protein L | 1 mg |
| 6530-10 | Protein L | 10 mg |
| 6530-100 | Protein L | 100 mg |
| 6530-5 | Protein L | 5 mg |
| 5530-100 | Protein L Antibody | 100 µg |
| 6537-1 | Protein L Magnetic Beads | 1 ml |
| 6531-1 | Protein L Sepharose | 1 ml |
| 6531-100 | Protein L Sepharose | 100 ml |
| 6531-25 | Protein L Sepharose | 25 ml |
| 6531-5 | Protein L Sepharose | 5 ml |
| 6538-1 | Protein L Sepharose Column | 1 ml |
| 6538-5 | Protein L Sepharose Column | 5 ml |
Protein A/G重組蛋白相關商品
| Catalog # | Product Name+ | Size |
| 6502-1 | Protein A/G | 1 mg |
| 6502-100 | Protein A/G | 100 mg |
| 6502-1000 | Protein A/G | 1 g |
| 6502-25 | Protein A/G | 25 mg |
| 6502-5 | Protein A/G | 5 mg |
| 6527-1 | Protein A/G Magnetic Beads | 1 ml |
| 6503-1 | Protein A/G Sepharose | 1 ml |
| 6503-100 | Protein A/G Sepharose | 100 ml |
| 6503-25 | Protein A/G Sepharose | 25 ml |
| 6503-5 | Protein A/G Sepharose | 5 ml |
| 6528-1 | Protein A/G Sepharose Column | 1 ml |
| 6528-5 | Protein A/G Sepharose Column | 5 ml |
| 6506-01 | Protein A/G-Biotin | 100 µg |
| 6506-1 | Protein A/G-Biotin | 1 mg |
| 6504-01 | Protein A/G-FITC | 100 µg |
| 6504-1 | Protein A/G-FITC | 1 mg |
Protein G/L重組蛋白相關商品
| Catalog # | Product Name+ | Size |
| 6540-1 | Protein A/G/L | 1 mg |
| 6540-100 | Protein A/G/L | 100 mg |
| 6540-1000 | Protein A/G/L | 1 g |
| 6540-25 | Protein A/G/L | 25 mg |
| 6540-5 | Protein A/G/L | 5 mg |
| 6547-1 | Protein A/G/L Magnetic Beads | 1 ml |
| 6541-1 | Protein A/G/L Sepharose | 1 ml |
| 6541-100 | Protein A/G/L Sepharose | 100 ml |
| 6541-25 | Protein A/G/L Sepharose | 25 ml |
| 6541-5 | Protein A/G/L Sepharose | 5 ml |
| 6548-1 | Protein A/G/L Sepharose Column | 1 ml |
| 6548-5 | Protein A/G/L Sepharose Column | 5 ml |