【R-PE |抗體標誌套組】INNOVA BIOSCIENCES 703-0010

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R-PE (R-Phycoerythrin) /抗體標誌套組
【R-PE 紅色螢光蛋白】 R-PE/抗體標誌套組,INNOVA BIOSCIENCES #703-0010 (100-200UG)
類別: R-PE螢光Dye抗體/蛋白質/胜肽(amino group)標誌
產品敍述: R-PE( fluorescent protein) 抗體標誌directly labeling kit (貨號#703-0010),首選英國innova biosciences公司專利研發之lightning-link抗體螢光標誌套組。市面上antibody labeling kit,在pH8~9環境下做反應。如此地環境易造成抗體活性失效,即使照著原廠提供的Protocol下進行反應,抗體回收的效能也很差; 太多抗體未被標誌,所以必須經由Dialysis及desalt步驟移去未結合的抗體。如此一來浪費了珍貴的抗體, 抗體結合效能也不理想。Lightning-link商品克服抗體標誌效能不理想的問題,專利研發改良,抗體標誌效能近乎100%,是標誌的首選商品。標誌抗體範圍10ug-1mg,大量標誌請來電洽詢02-86609496
生物標誌的專家innova biosciences
英國INNOVA BIOSCIENCES,以生物結合為主要事業。於1996年專利研發,中性環境下進行抗體結合反應,只要一個步驟,30秒hands on time。其抗體回收效能近似100%。不須再做Dialysis或desalt的步驟。Lightning-Link 只要一個步驟,即可將蛋白質/抗體/胜肽做標誌,具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合,因此無須desalt及dialysis,抗體標誌回收率100%。
Lightning-link抗體標誌原理
Lightning-Link以共價鍵結,辨視amino groups,性質極為穩定,一次反應後,可冷凍保存,節省實驗時間。
Lightning-link抗體標誌操作影片如下所示範
R-PE螢光蛋白特性
R-PE Label 498.544.566/580 (excitation/emission)
**R-PE excitation/emission光譜資料檔案下載**
R-Phycoerythrin (R-PE) is a fluorescent protein from the phycobiliprotein family, and is isolated from red algae. The absorbance spectrum of R-PE covers a broad wavelength range, which provides an advantage for multicolor immunofluorescent staining or flow cytometry as a number of excitation wavelengths can be used. R-PE is one of the most intensely fluorescent phycobiliproteins and has orange fluorescence. It is significantly brighter than conventional organic fluorophores. R-Phycoerythrin (R-PE) is a fluorescent protein from the phycobiliprotein family, present in red algae and cryptophytes. It has three maximal absorbance values of 498, 544 and 566nm (the optimal will depend on the application), and it has a strong emission peak at 580nm. RPE is closely related to B-Phycoerythrin (B-PE) and these are the most intense fluorescent phycobiliproteins providing an orange fluorescence. RPE conjugates are commonly used for immunofluorescence and flow cytometry and are ideal for multiple labeling with other fluorochromes.Click here to view other fluorescent proteins and dyes available in the Lightning-Link® range.
Lightning-link抗體標誌V.S.保存液
抗體保存於Azide、Gelatin及BSA溶液下,會干擾一般結合的效果,所以會再經過純化步驟,浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率,省下您寶貴的抗體。別於其它結合的方式,Lightning-Link無須在High pH的溶液下操作,所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體,都可反應。
Lightning-link抗體標誌應用
可以應用在ELISA. immunofluorescent. Flow. confocol. Western Blot等檢診及分子病毒之研究!!直接在一抗上做標誌 (可標誌螢光蛋白 螢光dye HRP Biotin等) ,克服二抗所造成的背景值過高及Host來源不同所造成Crosstalk的現象。
Lightning-link產品種類
**Lightning-link各種螢光光譜PDF資料下戴**
**Rapid Fluorescence Labeling Kits資料下載**
抗體標誌產品,可分為五大類產品
1. 酵素型標誌產品;HRP、AP、Glucose Oxidase等。
2. 螢光蛋白標誌產品;APC、PE、PerCP、RPE等螢光蛋白。
3. Biotin/Streptavidin標誌產品;Avidin、Streptavidin、Biotin等。
4. Tandem螢光標誌產品;應用FRET原理,使螢光emission於較遠的波段,例如APC/Cy5.5、PE/Cy7、PE/Cy5.5等。
Fluorescent dye Labeling Kits 螢光Dye/抗體標誌套組
Fluorescent Dye Labeling Kits
|
Lightning-Link®* |
Lightning-Link® Rapid* |
Maximal Absorbance |
Maximal Emission |
||
| AMCA |
352 |
452 |
|||
| DyLight® 350 |
354 |
432 |
|||
| Atto 390 |
388 |
468 |
|||
| DyLight® 405 |
402 |
428 |
|||
| PerCP |
484 |
678 |
|||
| PerCP/Cy5.5 |
484 |
692 |
|||
| DyLight® 488 |
496 |
524 |
|||
| Fluorescein |
498 |
532 |
|||
| R-Phycoerythrin |
498,544,566** |
580 |
|||
| PE/Texas Red |
498,544,566** |
618 |
|||
| PE/Atto594 |
498,544,566** |
632 |
|||
| PE/Cy5 |
498,544,566** |
672 |
|||
| PE/Cy5.5 |
498,544,566** |
700 |
|||
| PE/Cy7 |
498,544,566** |
782 |
|||
| Atto 488 |
504 |
530 |
|||
| B-Phycoerythrin |
546 |
580 |
|||
| Cyanine Dye 3 (Cy3) |
552 |
576 |
|||
| Rhodamine |
555 |
588 |
|||
| DyLight® 550 |
556 |
584 |
|||
| Atto 565 |
570 |
598 |
|||
| DyLight® 594 |
594 |
629 |
|||
| Texas Red® |
596 |
616 |
|||
| DyLight® 633 |
628 |
660 |
|||
| Atto 633 |
634 |
660 |
|||
| FluoProbes647H |
650 |
684 |
|||
| Cyanine Dye 5 (Cy5) |
652 |
678 |
|||
| Allophycocyanin |
652 |
666 |
|||
| APC/Cy5.5 |
652 |
700 |
|||
| APC/Cy7 |
652 |
790 |
|||
| DyLight® 650 |
656 |
686 |
|||
| Cyanine Dye 5.5 (Cy5.5) |
680 |
705 |
|||
| DyLight® 680 |
686 |
716 |
|||
| Atto 700 |
704 |
724 |
|||
| DyLight® 755 |
756 |
794 |
|||
| DyLight® 800 |
776 |
798 |
Lightning-link螢光蛋白抗體標誌/文獻期刊發表連結
Lightning-Link /期刊發表References
ATTO488抗體標誌套組訂購貨號資訊如下:
#貨號733-0030 (10-20UG) 反應3小時
#貨號733-0010 (100-200UG) 反應3小時
#貨號733-0015 (1-2MG) 反應3小時
#貨號350-0030 (10-20UG) 反應15分
#貨號350-0010 (100-200UG) 反應15分
#貨號350-0015 (1-2MG) 反應15分
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