活性白細胞介素 2 (IL2) | Cloud clone貨號APA073Hu02
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活性白細胞介素 2 (IL2) | Cloud clone貨號APA073Hu02
TCGF; Lymphokine; T-Cell Growth Factor; Aldesleukin
活性白細胞介素 2 (IL2) (Active Interleukin 2, IL2)
白血球介素-2 (Interleukin-2, IL2) 是由T細胞在抗原或促有絲分裂刺激下所產生的細胞激素。白血球介素-2 (Interleukin-2, IL2) 是免疫系統中的訊號分子，參與T細胞和B細胞的增殖以及其他調節免疫反應的關鍵活動。因此，為了檢測重組人白血球介素-2 (Interleukin-2, IL2) 的生物活性，製備脾臟單懸液，以刀豆球蛋白A（終濃度為3 ug/ml）活化。 72小時後收集細胞，用漢克斯洗滌。然後將小鼠脾臟淋巴細胞以10,000個/孔的密度接種到96孔板的三個孔中，並在加入或不加入不同濃度的重組人白血球介素-2 (Interleukin-2, IL2) 的情況下進行培養。培養96小時後，倒置顯微鏡觀察細胞，並以CCK-8試劑盒檢測細胞增生。每孔加入10 µl CCK-8 溶液，37 ℃孵育1-4小時後，以酶標儀在450nm處測定吸光度。倒置顯微鏡觀察IL-2作用96h後脾臟淋巴球增生情況，結果如圖1所示。重組人白血球介素-2 (Interleukin-2, IL2) 的量效曲線如圖2所示。結果顯示，重組人白血球介素-2 (Interleukin-2, IL2) 對脾臟淋巴球增生有明顯的促進作用，其EC50一般為0.088-0.092 ug/ml。
產品介紹: Cloud clone提供原核及真核生物表現之重組蛋白系列商品；其中白血球介素-2 (Interleukin-2, IL2) 為活性蛋白，Organism Species Homo sapiens (Human) 。純度> 95%，提供凍乾型式包裝(Freeze-dried powder)。可應用於Cell culture; Activity Assays等不同研究。
活性白細胞介素 2 (IL2) 蛋白質保存型式
蛋白質溶於10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. 避免vortex. 短期保存Store at 2-8°C for one month.分裝-80°C長期12 個月。請避免反覆冷凍解凍。
Figure 2. The dose-effect curve of IL-2 on Splenic lymphocytes cells.
活性白細胞介素 2 (IL2)活性重組蛋白相關之抗體 | 蛋白質 產品
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APA073Hu01 | Active Interleukin 2 (IL2) | Cell culture; Activity Assays. |
| APA073Hu02 | Active Interleukin 2 (IL2) | Cell culture; Activity Assays. |
| EPA073Hu61 | Eukaryotic Interleukin 2 (IL2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA073Hu01 | Recombinant Interleukin 2 (IL2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA073Hu61 | Active Interleukin 2 (IL2) | Cell culture; Activity Assays. |
| RPA073Hu02 | Recombinant Interleukin 2 (IL2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA073Hu01 | Polyclonal Antibody to Interleukin 2 (IL2) | WB; IHC; FCM |
| PAA073Hu06 | Polyclonal Antibody to Interleukin 2 (IL2) | WB |
| FAA073Hu01 | Anti-Interleukin 2 (IL2) Polyclonal Antibody | FCM |
| LAA073Hu71 | Biotin-Linked Polyclonal Antibody to Interleukin 2 (IL2) | WB; IHC; ICC. |
| MAA073Hu26 | Monoclonal Antibody to Interleukin 2 (IL2) | WB; IHC; ICC; IP. |
| MAA073Hu21 | Monoclonal Antibody to Interleukin 2 (IL2) | WB; IHC; ICC; IP. |
| MAA073Hu22 | Monoclonal Antibody to Interleukin 2 (IL2) | WB |
| MAA073Hu24 | Monoclonal Antibody to Interleukin 2 (IL2) | WB; IHC; ICC; IP. |
| MAA073Hu25 | Monoclonal Antibody to Interleukin 2 (IL2) | WB; IHC; ICC; IP. |
| MAA073Hu23 | Monoclonal Antibody to Interleukin 2 (IL2) | WB; IHC; ICC; IP. |
| MAA073Hu27 | Monoclonal Antibody to Interleukin 2 (IL2) | WB; IHC; ICC; IP. |
| MAA073Hu29 | Monoclonal Antibody to Interleukin 2 (IL2) | WB; IHC; ICC; IP. |
| HEA073Hu | High Sensitive ELISA Kit for Interleukin 2 (IL2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA073Hu | Mini Samples ELISA Kit for Interleukin 2 (IL2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA073Hu | ELISA Kit for Interleukin 2 (IL2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA073Hu | CLIA Kit for Interleukin 2 (IL2) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA073Hu | Multiplex Assay Kit for Interleukin 2 (IL2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA073Hu01 | ELISA Kit DIY Materials for Interleukin 2 (IL2) | Main materials for “Do It (ELISA Kit) Yourself”. |