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氯黴素 ELISA 試劑盒 | Chloramphenicol ELISA Kit貨號SL0054Ot

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氯黴素 ELISA 試劑盒 | Chloramphenicol ELISA Kit貨號SL0054Ot

This kit is Chloramphenicol Quantitative detection of residues in feed, fish, shrimp, tissue, egg, honey, milk, serum and urine sample.

產品描述:

This kit uses a direct competitive ELISA method in microplate coated with Chloramphenicol coupled antigen, adding Chloramphenicol standard or samples, free Chloramphenicol and pore strips Pre-coated Chloramphenicol conjugated antigen compete against Chloramphenicol antibody enzyme marker color with TMB substrate, stop solution added after the color from blue to yellow, with a microplate reader detection wavelength at 450nm, the sample absorbance value and Chloramphenicol content is inversely proportional to the sample through the standard curve Chloramphenicol content.

本試劑盒採用直接競爭ELISA法( direct competitive ELISA method),用於飼料、魚、蝦、組織、雞蛋、蜂蜜、牛奶、血清和尿液樣本中氯黴素殘留量的定量檢測。應用原理為: 在包被有氯黴素偶聯抗原(Chloramphenicol coupled antigen)的微孔板上加入氯黴素標準品(Chloramphenicol standard )或樣品、 free Chloramphenicol 和孔條,預包被的氯黴素偶聯抗原與TMB底物競爭氯黴素抗體酶標顯色,加入終止液後顏色由藍色轉為黃色,用酶標儀檢測氯黴素(Chloramphenicol) 含量。

網站規格小圖_工作區域 1_工作區域 1

Cat # +Size SL0054Ot
Size 96 tests
Species Reactivity Chloramphenicol ELISAKit uses a direct competitive ELISA method in microplate coated with
Chloramphenicol coupled antigen, adding Chloramphenicol standard or samples, free
Chloramphenicol and pore strips Pre-coated Chloramphenicol conjugated antigen compete against Chloramphenicol antibody enzyme marker color withTMB substrate, stop solution added after the color from blue to yellow, with a microplate reader detection wavelength at 4 5 0 nm, the sample absorbance value andChloramphenicol content is inversely proportional to the sample through the standardcurve Chloramphenicol content.
Applications This kit is Chloramphenicol Quantitative detection of residues in feed, fish, shrimp, tissue, egg, honey, milk, serum and urine sample.
Kit Parameters —This kit detection limit 0.05ppb. —B0 best value should be greater than 1.0 absorbance —Absorbance plate kit error of less than 8%, less than 15% between the plates. — With the manual extraction of tissue samples provided by more than 80%recoveryrate.
Kit Components

( 1) Pre-coated Chloramphenicol coupled antigen removable microtiter plates: 1
block ( 12 wells × 8 strips)
(2) Chloramphenicol Standard: 6 bottles ( 1ml / vial), contents were: 0 ppb, 0.05ppb,0.15ppb, 0.45ppb,1.35ppb, 4.05ppb. Anti- Chloramphenicol antibody conjugate: 1 bottle (6ml)
(4) Color liquid A: 1 bottle (6ml)
(5) Color liquid B: 1 bottle (6ml)
(6) Stop Solution: 1 bottle (6ml), 2M sulfuric acid. (7) Sample Diluent: 1 bottle ( 10 × , 6ml), used for sample dilution. (8) washing liquid concentrate: 1 bottle (20 × , 20ml), for the washer
(9) A description

 

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