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黃麴毒素(AFT)Elisa試劑盒 | Aflatoxin(AFT) Elisa kit 貨號SL0004Ot

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黃麴毒素(AFT)Elisa試劑盒操作手冊

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黃麴毒素(AFT)Elisa試劑盒 | Aflatoxin(AFT) Elisa kit 貨號SL0004Ot

This kit is Aflatoxin(AFT) Quantitative detection of residues in feed, fish, shrimp, tissue, egg, honey, milk, serum and urine sample.

產品描述:

This kit uses a direct competitive ELISA method in microplate coated with AFT coupled antigen, adding AFT standard or samples, free AFT and pore strips
Pre-coated AFT conjugated antigen compete against AFT antibody enzymemarker color with TMB substrate, stop solution added after the color fromblue to yellow, with a microplate reader detection wavelength at 450nm, the sampleabsorbance value and AFT content is inversely proportional to the sample throughthestandard curve AFT content.

本試劑盒採用直接競爭 ELISA 方法(direct competitive ELISA method),在塗有 AFT 偶聯抗原(AFT coupled antigen)的微孔板上添加 AFT 標準品(AFT standard)或樣本、 free AFT 和孔條預包覆的AFT標記抗原與TMB底物競爭AFT抗體酶標顯色,加入終止液後顏色由藍色變為黃色,用酶標儀檢測波長450nm,透過標準曲線繪製樣本吸光度值與AFT含量成反比。

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Cat # +Size SL0004Ot
Size 96 tests
Species Reactivity This kit uses a direct competitive ELISA method in microplate coated with AFTcoupled antigen, adding AFT standard or samples, free AFT and pore strips
Pre-coated AFT conjugated antigen compete against AFT antibody enzymemarker color with TMB substrate, stop solution added after the color fromblue to yellow, with a microplate reader detection wavelength at 450nm, the sampleabsorbance value and AFT content is inversely proportional to the sample throughthestandard curve AFT content. Kit provided by the standard curve range 0.1ppb~8.1ppb.
Applications This kit is Aflatoxin(AFT) Quantitative detection of residues in feed, fish, shrimp, tissue, egg, honey, milk, serum and urine sample.
Features & Benefits —This kit detection limit 0.3ppb. —B0 best value should be greater than 1.0 absorbance —Absorbance plate kit error of less than 8%, less than 15% between the plates. —With the manual extraction of tissue samples provided by more than 80%recoveryrate.
Kit Components

(1) Pre-coated AFT coupled antigen removable microtiter plates: 1 block (12 wells × 8 strips)

(2) AFT Standard: 6 bottles (1ml / vial), contents were:  0 ppb, 0.1 ppb,0.3 ppb,0.9 ppb,2.7 ppb,8.1 ppb.

Anti- AFT antibody conjugate: 1 bottle (6ml)

 (4) Color liquid A: 1 bottle (6ml)

(5) Color liquid B: 1 bottle (6ml)

(6) Stop Solution: 1 bottle (6ml), 2M sulfuric acid.

(7) Sample Diluent: 1 bottle (10 ×, 6ml), used for sample dilution.

(8) washing liquid concentrate: 1 bottle (20 ×, 20ml), for the washer

(9) A description

 

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