乙醇測定試劑盒| Ethanol Assay Kit 貨號Abnova™KA4784
| 代理廠牌： | |
| 原廠連結： | |
| 相關下載： |
乙醇測定試劑盒| Ethanol Assay Kit 貨號Abnova™KA4784
100 assays
酒精飲料(Alcoholic drinks )是日常飲用的飲料之一。研究顯示大量飲酒可能導致各種形式的肝臟疾病( liver diseases)並增加死亡率。定量測定酒精（乙醇，C2H5OH）在基礎研究、藥物發現、臨床研究和酒精工業。用於測量乙醇濃度的簡單、直接和自動化的程序是非常理想的。乙醇測定試劑盒(Ethanol Assay Kit, 貨號KA4784) 基於乙醇脫氫酶催化乙醇氧化，其中形成NADH 與甲臢 (MTT) 髮色團偶聯。在 565 nm 處測量的產品顏色強度，與樣本中的乙醇濃度成正比。
✓ 應用
● 直接測定：血清、血漿、尿液和唾液樣本中的乙醇。
● 藥理學：藥物對酒精代謝的影響。
✓ 特點
● 靈敏、準確。檢測極限 0.0008 vol %（140 µM 或 8 ppm），乙醇溶液中線性度高達 0.1% 96 孔板測定。
● 方便。過程包括添加工作試劑、孵育30分鐘並停止反應。無需 37°C 加熱器。
● 高通量。可輕鬆自動化，作為高通量 96 孔板檢測，可進行數千次檢測每天樣品
Product Description
Ethanol Assay Kit is a colorimetric determination of ethanol at 565 nm.
Suitable Sample
Plasma, Saliva, Serum, Urine
Sample Volume
10 uL
Detection Method
Colorimetric
Calibration Range
0.0008 to 0.1%
Limit of Detection
0.0008%
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard Curve
The standard curve is for the purpose of demonstration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store the kit at -20°C.
- Sulforaphane alleviates ethanol-mediated central inhibition and reverses chronic stress-induced aggravation of acute alcoholism via targeting Nrf2-regulated catalase expression.
Jun-Feng Xu, Jia-Jing Lu, Yu Cao, Wen Wang, Hou-Hong Li, Jian-Guo Chen, Fang Wang, Peng-Fei Wu.
Neuropharmacology 2020 Jul; 176:108235.
Application：Quant, Mouse, Blood.
- Ethanol promotes alcohol-related colorectal cancer metastasis via the TGF-β/RUNX3/Snail axis by inducing TGF-β1 upregulation and RUNX3 cytoplasmic mislocalization.
Zheng K, Yu J, Chen Z, Zhou R, Lin C, Zhang Y, Huang Z, Yu L, Zhao L, Wang Q.
EBioMedicine 2019 Dec; 50:224.
Application：Func, Mouse, Mouse serum.