CheKine™ 微量總穀胱甘肽 (T-GSH) 檢測試劑盒 | Toal Glutathione (T-GSH) Assay Kit KTB1670 

CheKine™ Micro Toal Glutathione (T-GSH) Assay Kit   (0.04-4 ug/mL)

CheKine™ Micro Pyruvate Acid (PA) Assay Kit
穀胱甘肽(Glutathione)是由麩胺酸( glutamate)、半胱胺酸(cysteine)和甘胺酸(glycine)組成的三肽。穀胱甘肽(Glutathione)含有巰基，具有抗氧化和綜合解毒作用。穀胱甘肽有還原型（GSH）或氧化型(oxidized form, GSSG），在生理條件下以還原型穀胱甘肽為主。穀胱甘肽還原酶催化兩種形式之間的交替。 GSH/GSSG 是細胞中最重要的氧化還原對之一。因此，細胞內GSH和GSSG含量以及GSH/GSSG比值的測定可以很好地反映細胞的氧化還原狀態，也是穀胱甘肽氧化還原循環的主要指標之一。

Product name	CheKine™ Micro Toal Glutathione (T-GSH) Assay Kit
Applications notes	GSSG is reduced to GSH using Glutathione Reductase (GR). GSH reacts with 5,5′-dithiobis-bis-(2-nitrobenoicacid) to form 2-nitrobenoicacid which has a characteristic absorption peak at 412 nm, the content of total glutathione is determined then. It can test samples of tissues, bacteria, fungus, cells, serums, ect.
Alternative	Toal Glutathione; T-GSH; t-GSH
Kit components	• Extraction Buffer • Assay Buffer • GR • GR Cofactor • Chromogen • Standard
Features & Benefits	• Determination of the content of total glutathione in a variety of biological samples such as tissues, bacteria, fungus, cells, serums, ect. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. • Standard and standard curve line are provided.
Usage notes	• Do not mix or substitute reagents or materials from other kit lots or different vendors, or it may cause abnormal result. •Avoid foaming or bubbles when mixing or reconstituting components. • To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. •Ensure all reagents and solutions are at the appropriate temperature before starting the assay. •Pls dilute the sample furtherly when the concentration of sample is higher than the maximal concentration of standard.
Storage instructions	Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Glutathione is a tripeptide consisting of glutamate, cysteine and glycine. Glutathione contains sulfhydryl groups and has antioxidant and integrated detoxification effects. Glutathione has reduced form (GSH) or oxidized form (GSSG), reduced glutathione predominates in physiological conditions. Glutathione reductase catalyzes the alternations between the two forms. GSH/GSSG is one of the most important redox pairs in cells. Therefore, the determination of GSH and GSSG content and GSH/GSSG ratio in cells can well reflect the redox state of cells and is also one of the main indicators of the glutathione redox cycle.
Alternative	Toal Glutathione; T-GSH; t-GSH

 

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