蘇丹紅 (SUD) 檢測-用於番茄汁、番茄醬、辣椒（參巴）、辣椒粉、飼料、雞蛋中SUD的定量檢測 (0.3 ppb)

蘇丹紅 (SUD) ELISA 檢測試劑盒 | Sudan Red (SUD) ELISA Kit  Abbexa貨號abx364828
Sudan Red (SUD) ELISA Kit

蘇丹 I (Sudan I)（通常也稱為 CI 溶劑黃 14 和溶劑橙 R）是一種有機化合物(organic compound)，通常歸類為偶氮染料(azo dye)。它是一種深橙紅色固體(intensely orange-red solid)，可添加到蠟(colourise waxes)、油(oils)、汽油(petrol)、溶劑(solvents)和拋光劑(polishes)中著色。蘇丹紅I(Sudan I)也被用於各種食品的著色，特別是咖哩粉(curry powder )和辣椒粉(chili powder)，儘管現在許多國家禁止在食品中使用蘇丹 I (Sudan I)，因為蘇丹 I (Sudan I)、蘇丹紅III(Sudan III)和蘇丹紅IV(Sudan IV )已被列為第3類致癌物（未依其對人類的致癌性分類）由國際癌症研究機構分類。蘇丹 I (Sudan I)仍然用於一些橙色煙霧配方中，並作為化學實驗中使用的棉花垃圾的著色劑。

檢測極限：番茄汁/番茄醬 – 12 ppb、辣椒醬 – 12 ppb、雞蛋（雞/鴨/鵝） – 30 ppb、辣椒
粉末/飼料 – 120 ppb
交叉反應性：SUD – 100%，對位紅 – 123%，羅丹明 – 8%

此測定具有競爭性，因此樣品中的 SUD 濃度與吸光度之間存在負相關測量。使用標準濃度（y 軸）和測量的吸光度（x 軸）的對數建立圖表。應用最佳擬合趨勢線穿過標準點。使用此圖根據 OD 值計算樣品濃度。如果樣品有稀釋後，從標準曲線上讀取的濃度必須乘以稀釋倍數。應用：用於番茄汁、番茄醬、辣椒（參巴）、辣椒粉、飼料、雞蛋中SUD的定量檢測。靈敏度：0.3 ppb（奈克/毫升）

樣品前處理 24 小時內將待檢測的樣品儲存在 2-8°C 下。或者，等分並長期保存在 -20°C 或 -80°C 下。避免重複凍融循環。

• 番茄汁、番茄醬或辣椒醬：秤取2 ± 0.05 g 勻漿並加入離心管中。加入 10 毫升甲醇並混合 5 分鐘。室溫下 4000 RPM 離心 10 分鐘，收集上層相。加入 100 µl將此液體加入 700 μl 去離子水中。充分混合並取 50 μl 混合物進行分析。註：樣品稀釋倍數：40，最小檢測劑量：12 ppb。

• 雞蛋：低速均質雞蛋樣本。秤取 1 ± 0.05 g 勻漿並加入離心管中。添加9毫升甲醇並充分混合 5 分鐘。 15°C、4000 RPM 離心 10 分鐘，收集上層相。加入 100 µl 此液體
900μl去離子水。充分混合並取 50 μl 混合物進行分析。註：樣品稀釋倍數：100，最小檢測劑量：30 ppb。

• 辣椒粉/飼料：秤取1 ± 0.05 g 勻漿並加入離心管中。加入10毫升甲醇並充分混合5分鐘。室溫下 4000 RPM 離心 10 分鐘，收集上層相。將 20 µl 此液體加入至 780 µl去離子水。充分混合並取 50 μl 混合物進行分析。註：樣品稀釋倍數：400，最小檢測劑量：120 ppb

Introduction	Sudan I (also commonly known as CI Solvent Yellow 14 and Solvent Orange R), is an organic compound, typically classified as an azo dye. It is an intensely orange-red solid that is added to colourise waxes, oils, petrol, solvents, and polishes. Sudan I has also been adopted for colouring various foodstuffs, especially curry powder and chili powder, although the use of Sudan I in foods is now banned in many countries, because Sudan I, Sudan III, and Sudan IV have been classified as category 3 carcinogens (not classifiable as to its carcinogenicity to humans) by the International Agency for Research on Cancer. Sudan I is still used in some orange-coloured smoke formulations and as a colouring for cotton refuse used in chemistry experiments.
Target	Sudan Red (SUD)
Reactivity	General (All species)
Tested Applications	ELISA
Recommended dilutions	Optimal dilutions/concentrations should be determined by the end user.
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit’s manual.
Validity	The validity for this kit is at least 6 months. Up to 12 months validity can be provided on request.
Stability	The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Test Range	0.3 ppb – 24.3 ppb
Sensitivity	0.3 ppb
Standard Form	Lyophilized
Detection Method	Colorimetric
Assay Type	Competitive
Assay Data	Quantitative
Sample Type	Tomato juice, ketchup, chilli sauce, chilli powder, egg and feed.
Kit Components	Please refer to the kit manual.
Material Required But Not Provided	Please refer to the kit manual.
Sample Collection/Preparation	For target-specific sample preparation procedures, please refer to the kit manual.
Reagent Preparation	For this kit’s reagent preparation procedure, please refer to the kit manual.
Assay Procedure	For this kit’s assay procedure, please refer to the kit manual.
Availability	Shipped within 5-12 working days.
Note	This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Plate coated with	Antigen