Type I Bovine Collagen | 貨號1200-02/1200-02S (凍乾粉/溶液)

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Type I Bovine Collagen | 貨號1200-02/1200-02S (凍乾粉/溶液) 規格0.5 mg/mL
細胞免疫專家極力推薦SouthernBiotech高品質膠原– Type I Collagen
關於膠原(Collagen):
膠原蛋白是動物結締組織重要的蛋白質,在哺乳動物中的含量尤為豐富,佔有機體蛋白質百分之25%-35%含量。膠原是細胞外最重要的水不溶性纖維蛋白, 是構成細胞外基質(extracellular matrix, ECM)的骨架。膠原在細胞外基質中形成半晶體的纖維,給細胞提供抗張力和彈性,並在細胞的遷移和發育中起作用。膠原蛋白的基本結構單位是原膠原(tropocollagen), 原膠原肽鏈的一級結構具有(Gly-x-y)n重複序列, 其中x常為脯氨酸(Pro), y常為羥脯氨酸(Hypro)或羥賴氨酸(Hylys)。Hylys殘基可發生糖基化修飾,其糖單位有的是一個半乳糖殘基(Gal), 但通常是二糖(Glu-Gal-),膠原上的糖所占的量約為膠原的10%。原膠原是由三條α-肽鏈組成的纖維狀蛋白質, 相互擰成三股螺旋狀構型, 長300nm, 直徑1.5nm。目前已發現29種不同組織中不同類型的膠原; 不同類型的膠原定位於體內的特定組織,也有2-3種不同的膠原存在於同一組織中。而機體內90%的膠原為I、II、III、IV型。
膠原蛋白(Collagen)是細胞外空間的主要結構蛋白,也是 ECM 中最豐富的蛋白質。膠原蛋白分為兩類 – 原纖維( fibril) I、II、III、V 型)和非原纖維(non-fibril)(IV、VI 型)。 I 型膠原蛋白(Type I collagen)是最豐富的膠原蛋白,幾乎在所有結締組織中表達,包括皮膚(skin)、肌腱(tendon)和骨組織(bone tissue)。它也是間隙基質(interstitial matrix)的主要成分。 I 型膠原蛋白突變與一系列疾病有關,包括成骨不全症(osteogenesis imperfecta)和埃勒斯-當洛斯症候群(Ehlers–Danlos syndrome)。 I 型膠原蛋白由兩條 α1(I) 鏈和一條 α2(I) 鏈組成。
| Description | Collagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes – fibril (types I, II, III, V) and non-fibril (types IV, VI). Type I collagen is the most abundant collagen and is expressed in almost all connective tissues including skin, tendon, and bone tissue. It is also the predominant component of the interstitial matrix. Type I collagen mutations are associated in a range of diseases including osteogenesis imperfecta and Ehlers–Danlos syndrome. Type I collagen consists of two α1(I) chains and one α2(I) chain. |
|---|---|
| Source | Placental villi |
| Purity | > 90% by SDS-PAGE |
| Purification Method | Controlled and limited pepsin digestion followed by selective salt precipitation |
| Buffer Formulation | 500 mM acetic acid |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications | ELISA – Quality tested SDS-PAGE – Quality tested Surface Plasmon Resonance – Reported in literature 2 Coating Material for – Adhesion Studies – Reported in literature 1-3 Migration Studies – Reported in literature 4 |
- 1. Switalski LM, Butcher WG, Caufield PC, Lantz MS. Collagen mediates adhesion of Streptococcus mutans to human dentin. Infect Immun. 1993;61:4119-25. (Coating, Adhesion Studies)
- 2. House-Pompeo K, Boles JO, Höök M. Characterization of bacterial adhesin interactions with extracellular matrix components utilizing biosensor technology. Methods. 1994;6:134-42. (SPR, Coating, Adhesion Studies)
- 3. McGrady JA, Butcher WG, Beighton D, Switalski LM. Specific and charge interactions mediate collagen recognition by oral lactobacilli. J Dent Res. 1995;74:649-57. (Coating, Adhesion Studies)
膠原蛋白
| 產品名稱 | 編號(cat#) | 包裝形式 | 規格 |
| Type I Human Collagen | 1200-01S | 溶液 | 0.50mg |
| Type I Bovine Collagen | 1200-02/1200-02S | 凍乾粉/溶液 | 0.25mg/0.50mg |
| Type I Rat Collagen | 1200-03/1200-03S | 凍乾粉/溶液 | 0.25mg/0.50mg |
| Type II Rat Collagen | 1220-02/1220-02S | 凍乾粉/溶液 | 0.25mg/0.50mg |
| Type III Human Collagen | 1230-01S | 溶液 | 0.50mg |
| Type III Bovine Collagen | 1240-02S | 溶液 | 0.50mg |
| Type IV Human Collagen | 1250-01S | 溶液 | 0.50mg |
| Type IV Mouse Collagen | 1250-04S | 溶液 | 0.50mg |
| Type IV Bovine Collagen | 1260-02 | 凍乾粉 | 0.25mg |
| Type V Human Collagen | 1270-01/1270-01S | 凍乾粉/溶液 | 0.1mg/0.25mg |
| Type V Bovine Collagen | 1280-02S | 溶液 | 0.25mg |
| Type VI Human Collagen | 1290-01S | 溶液 | 0.25mg |
| Type VI Bovine Collagen | 1300-02/1300-02S | 凍乾粉/溶液 | 0.10mg/0.25mg |
膠原蛋白多株抗體
| 產品名稱 | 編號(cat#) | 包裝形式 | 規格 |
| Goat Anti-Type I Collagen | 1310-01/1310-08 | 未標記/生物素化 | 0.2mg/0.2mg |
| Goat Anti-Type II Collagen | 1320-01/1320-08 | 未標記/生物素化 | 0.2mg/0.2mg |
| Goat Anti-Type III Collagen | 1330-01/1330-08 | 未標記/生物素化 | 0.2mg/0.2mg |
| Goat Anti-Type IV Collagen | 1340-01/1340-08 | 未標記/生物素化 | 0.2mg/0.2mg |
| Goat Anti-Type V Collagen | 1350-01/1350-08 | 未標記/生物素化 | 0.2mg/0.2mg |
| Goat Anti-Type VI Collagen | 1360-01/1360-08 | 未標記/生物素化 | 0.2mg/0.2mg |
膠原蛋白單株抗體
| 產品名稱 | 編號(cat#) | 包裝形式 | 規格 |
| Mouse Anti-Human Type I Collagen | 1440(1)-01 | 未標記 | 0.1mg |
| Mouse Anti-Human Type IV Collagen | 1460-01 | 未標記 | 0.1mg |
| Mouse Anti-Human Type IV Collagen | 1460-02 | FITC | 0.1mg |
| Mouse Anti-Human Type IV Collagen | 1460-08 | BIOT | 0.1mg |
| Mouse Anti-Human Type IV Collagen | 1460-09 | R-PE | 0.1mg |
應用: ELISA、免疫螢光、免疫細胞化學、免疫組織化學(冰凍切片)
Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |
