Griess Reagent Kit | Biotium 貨號#30100 (2500個反應) 

Nitrite concentrations in the samples should be within the linear range of the assay (~1-100 uM).

Griess 試劑用於生物樣本（例如細胞裂解液或組織灌流液）中基於吸光度的亞硝酸鹽檢測。本試劑含有兩種化學物質：磺胺酸(sulfanilic acid )和N-(1-萘二胺) (N-(1-naphthalenediamine))。在酸性條件(acidic conditions )下，磺胺酸(sulfanilic acid)被亞硝酸鹽(nitrite)轉化為重氮鹽(diazonium salt)，它很容易與N-(1-萘二胺)(N-(1-naphthalenediamine)) 偶聯，形成高度著色的偶氮染料(azo dye)，可透過548 nm 處的吸光度進行測量。生理條件下，NO不穩定，很快就被氧化成亞硝酸鹽(nitrite)和硝酸鹽(nitrate)的混合物。為了間接測量亞硝酸鹽中的 NO 水平，在進行測定之前，必須使用硝酸鹽還原酶（不包括在內）將硝酸鹽酶(nitrate reductase)促還原為亞硝酸鹽，以便測量亞硝酸鹽的總量。

Griess Reagent Kit優點

• Detect and measure nitrite in biological samples
• Can determine NO levels by adding a nitrate reduction step
• Measure by absorbance at 548 nm
• Solution contains sulfanilic acid and N-(1-naphthalenediamine)
• Enough reagents for 500 cuvette assays or 2500 microplate assays

1. Materials required but not provided

• Nitrate Reductase ≥300 U/g (Sigma catalog no. N7265)

• L-Glutamic Dehydrogenase from bovine liver ≥20 U/mg (Sigma catalog no. G7882)

• β-NADPH (Sigma catalog no. N1630)

• α-Ketoglutaric acid

• Ammonium chloride (NH4 Cl)

• 10X Phosphate-Buffered Saline (Biotium catalog no. 22020)

Biotium 提供範圍廣泛的螢光探針用於研究凋亡細胞(Apotosis)

The NucView® Caspase-3 Substrate comes in 3 colors, and measures caspase activity in real time, in live cells. MitoView™ 633 can measure the decrease in mitochondria membrane potential that occurs in early apoptosis. CF® Dye Annexin V conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes.