SweTransRNA 轉染試劑(SweTransRNA Transfection Reagent) | SERVICEBIO 貨號G1806-0.5ML
SERVICEBIO 細胞分析>細胞轉染系列產品- SweTransRNA Transfection Reagent
SweTransRNA 轉染試劑(SweTransRNA Transfection Reagent) | SERVICEBIO 貨號G1806-0.5ML
(SweTransRNA Transfection Reagent)
產品介紹:
本產品是一種高性能的基於陽離子聚合物的RNA轉染試劑，可將RNA直接遞送至靶細胞的細胞質，不被核酸酶降解。基因表現過程發生在細胞質中，不進入細胞核，不受轉錄限制。本產品具有受血清影響小、毒性極低、穩定性好、操作簡便、重複性好等優點，可應用於各種貼壁細胞及懸浮細胞的RNA轉染。
This product is a high-performance cationic polymer-based RNA transfection reagent that delivers RNA directly to the cytoplasm of target cells without being degraded by nucleases.The gene expression process takes place in the cytoplasm without entering the nucleus and is not restricted by the transcriptional regulation of the target cell.This product has the advantages of little influence by serum, extremely low toxicity, good stability, easy operation and good repeatability.It can be applied to RNA transfection of various adherent cells and suspension cells.
| Component | G1806-0.5ML |
| SweTransRNA Transfection Reagent | 0.5 mL |
| Manual | 1 pc |
使用用法:
1.貼壁細胞轉染前準備（以6孔板為例，其他規格請參考附表）：
a.轉染前一天，將細胞以適當的密度均勻種植於孔板中，使正式轉染時細胞收斂至70-85%；
b.轉染前，移除原細胞培養基，以室溫PBS緩衝液洗滌細胞1-2次，再更換為1.9 mL無血清或低血清（低於5%）基礎培養基（不同細胞有不同的血清依賴性，請酌情調整血清百分比）；
c.換液並製備轉染複合物後將細胞置於細胞培養箱中；
2. 轉染用懸浮細胞的製備：
a.轉染當天，離心適量細胞除去培養基後，將細胞重懸於無血清或低血清（5%以下）基礎培養基中至適當濃度，均勻接種1.9 mL重懸浮液在孔板中；
b.將接種有懸浮細胞的孔板放入細胞培養箱中，開始製備轉染複合物（轉染複合物與培養基的體積比可參考貼壁細胞的比例適當調整）；
3. 轉染複合物的製備：
a.配製A液：50 μL無血清基礎培養基，加80 pmol RNA，吹勻混勻；
b. B液配製：50μL無血清基礎培養基加4-20μL SweTransRNA轉染試劑，吹勻混勻；
c. A液和B液在室溫下放置5分鐘；
d.將B液加入A液中，輕輕吹混均勻，繼續室溫孵育15-20分鐘，形成RNA-轉染試劑複合體；
4. 細胞轉染：
a.將上一步驟得到的RNA-轉染試劑複合物加入孔板中，並更換「步驟1」或「步驟2」中的液體；
b.輕輕搖晃培養板，誘導含有轉染複合物的培養基與孔板中的培養基充分混合後，將孔板置於37℃、5%CO2培養箱中培養；
c.培養4-6小時後，將不同細胞換入對應的新鮮培養基，放入培養箱中進一步培養；
d.轉染24-48小時後，觀察或收集細胞。
1. Pre-transfection preparation of adherent cells (6-well plate for example, other specifications refer to the attached table):
a. One day before transfection, the cells are planted uniformly in the well plate at a suitable density so that the cells converge to 70-85% during formal transfection;
b. Before transfection, remove the original cell culture medium, wash the cells with room temperature PBS buffer for 1-2 times, and then replace with 1.9 mL of serum-free or low-serum (less than 5%) basal medium (different cells have different serum dependency, please adjust the serum percentage as appropriate);
c. Cells are placed in a cell culture incubator after fluid exchange and transfection complexes are prepared;
2. Preparation of suspension cells for transfection:
a. On the day of transfection, after centrifuging the appropriate amount of cells to remove the medium, resuspend the cells in serum-free or low-serum (5% or less) basal medium to the appropriate concentration, and inoculate 1.9 mL of the resuspension uniformly in the well plate;
b. Place the well plate inoculated with suspended cells in a cell culture incubator and start to prepare the transfection complex (the volume ratio between the transfection complex and the culture medium can be adjusted appropriately by referring to the ratio of adherent cells);
3. Transfection complex preparation:
a. Prepare Solution A: 50 μL of serum-free basal medium with 80 pmol of RNA, blown up and mixed;
b. Preparation of solution B: 50 μL of serum-free basal medium with 4-20 μL of SweTransRNA transfection reagent, blown up and mixed;
c. Leave Liquid A and Liquid B at room temperature for 5 minutes;
d. Add solution B to solution A, gently blow to mix well, and continue to incubate at room temperature for 15-20 min to form the RNA-transfection reagent complex;
4. Transfection of cells:
a. The RNA-transfection reagent complex obtained in the previous step is added to the orifice plate with the liquid replaced in “Step 1” or “step 2”;
b. After gently shaking the culture plate to induce the medium containing the transfection complex to mix well with the medium in the well plate, the well plate is incubated at 37°C in a 5% CO2 incubator;
c. After 4-6 h of incubation, the different cells are changed into the corresponding fresh medium and placed in the incubator for further incubation;
d. After 24-48 h of transfection, cells are observed or collected.
注意事項
1. 請使用狀態良好的健康細胞，復甦細胞建議在轉染前至少傳代兩次。
2. 請使用高品質RNA進行轉染。
3.產品首次使用，建議單獨包裝，避免重複凍融。
4.在細胞耐受的前提下，可適當延長RNA-轉染試劑複合物的孵育時間，轉染效果較好。RNA與轉染試劑的比例可酌情調整。
5.為了您的健康與安全，操作時請穿戴實驗服和手套。
1. Please use healthy cells in good condition, and resuscitated cells are recommended to be passaged at least twice before transfection.
2. Please use high quality RNA for transfection.
3. The product is used for the first time, it is recommended to pack separately to avoid repeated freezing and thawing.
4. Under the premise of cell tolerance, the incubation time of RNA-transfection reagent complex can be extended appropriately, and the transfection effect is better.The ratio of RNA to transfection reagents can be adjusted as appropriate.
5. For your health and safety, please wear lab coat and gloves during operation.
|
G1802-10ML
|
PEI 40K Transfection Reagent
|
10 mL
|
|
G1802-1ML
|
PEI 40K Transfection Reagent
|
1 mL
|
|
G1803-1ML
|
Polybrene (10 mg/mL)
|
1 mL
|
|
G1805-0.5ML
|
SweTransDNA Transfection Reagent
|
0.5 mL
|
|
G1806-0.5ML
|
SweTransRNA Transfection Reagent
|
0.5 mL
|
Servicebio台灣品牌介紹-BRAND/AGENCY/品牌介紹- Servicebio
| Classfication | Cat No. | Name | Place of Origin | Spec. |
|
Amino Acids
|
GC304002 | L-Glutamine | 產品資料 | 100 g |
| GC304003 | L-Arginine hydrochloride | 產品資料 | 25 g | |
| GC304004-100g | L-Glutamine acid | 產品資料 | 100 g | |
| GC304005 | L-Isoleucine | 產品資料 | 25 g | |
| GC304006-25g | L-Leucine | 產品資料 | 25 g | |
| GC304007 | L-Lysine hydrochloride | 產品資料 | 25 g | |
| GC304009 | L-Methionine | 產品資料 | 25 g | |
| GC304010 | L-Phenylalanine | 產品資料 | 10 g | |
| GC304011-25g | L-Alanine | 產品資料 | 25 g | |
| GC304012 | L-Histidine | 產品資料 | 25 g | |
| GC304013 | L-Serine | 產品資料 | 25 g | |
| GC304014 | L-tryptophan | 產品資料 | 5 g | |
| GC304016 | L-Threonine | 產品資料 | 25 g | |
| GC304017 | L-Valine | 產品資料 | 10 g | |
| GC304019 | Glycine | 產品資料 | 500 g | |
| GC304021 | L-Histidine hydrochloride monohydrate | 產品資料 | 25 g | |
| GC304022 | Glycine cell culture grade 甘氨酸Glycine (細胞培養等級 |
產品資料 | 100 g | |
|
Protein Derivatives
|
GC305002-5g | Iodoacetamide | 產品資料 | 5 g |
| GC305003 | Trypsin 1:250 Source: Porcine Pancreas | 產品資料 | 10 g | |
| GC305004-5g | Pepsin 1:3000 | 產品資料 | 5 g | |
| GC305005 | Horseradish peroxidase | 產品資料 | 1 g | |
| GC305006 | Bovine Serum Albumin – import | 產品資料 | 100 g | |
| GC305009 | Human Albumin-FITC | 產品資料 | 0.5 mL | |
| GC305010 | Bovine Serum Albumin BSA | 產品資料 | 100 g | |
| GC305010-25G | Bovine Serum Albumin BSA | 產品資料 | 25 g | |
| GC305010-50G | Bovine Serum Albumin BSA | 產品資料 | 50 g | |
| GC305010-5G | Bovine Serum Albumin BSA | 產品資料 | 5 g | |
| GC305013-100mg | Collagenase I | 產品資料 | 100 mg | |
| GC305014-100mg | Collagenase II | 產品資料 | 100 mg | |
| GC305015-100mg | Collagenase IV 膠原酶IV (Collagenase IV) |
產品資料 | 100 mg | |
| GC305016-100g | Gelatine | 產品資料 | 100 g | |
|
Hormones
|
GC302002-25mg | Abscisic acid | 產品資料 | 25 mg |
| GC302003 | Angiotensin II | 產品資料 | 10 mg | |
|
Biological pigments
|
GC307002-25g | Crystal violet | 產品資料 | 25 g |
| GC307003-5g | Trypan Blue | 產品資料 | 5 g | |
| GC307005 | Alcian Blue8GX | 產品資料 | 1 g | |
| GC307006-5g | Bromphenol Blue | 產品資料 | 5 g | |
| GC307007 | Serva blue G-250 | 產品資料 | 10 g | |
| GC307008-5g | Congo Red | 產品資料 | 5 g | |
| GC307009-25g | Alizarin Red S | 產品資料 | 25 g | |
| GC307010-1g | Giemsa′s Stain | 產品資料 | 1 g | |
| GC307011 | Fuchsin basic | 產品資料 | 5 g | |
| GC307012-10g | Ponceau S | 產品資料 | 10 g | |
| GC307013 | Acid red 26 2R | 產品資料 | 5 g | |
| GC307014 | Direct Red 80 | 產品資料 | 5 g | |
| GC307015-5g | Sudan Black B | 產品資料 | 5 g | |
| GC307017 | Aniline blue | 產品資料 | 5 g | |
| GC307018 | Nuclear fast red | 產品資料 | 5 g | |
| GC307019 | Solvent Blue 38 | 產品資料 | 5 g | |
| GC307020 | Hematoxylin | 產品資料 | 5 g | |
| GC307021 | Solvent Red 43 | 產品資料 | 10 g | |
| GC307022 | Eosin Y, water soluble | 產品資料 | 10 g | |
| GC307023 | Safranin O | 產品資料 | 10 g | |
| GC307024 | Fast Green | 產品資料 | 10 g | |
| GC307026-5g | Pyronin G | 產品資料 | 5 g | |
| GC307027-1g | Janus Green B | 產品資料 | 1 g | |
| GC307030-10g | Brilliant Cresyl Blue | 產品資料 | 10 g | |
| GC307031 | Coomassie brilliant blue R-250 | 產品資料 | 5 g | |
| GC307034-1g | Thionine acetate | 產品資料 | 1 g | |
| GC307035-1g | Cresyl Fast Violet | 產品資料 | 1 g | |
| GC307036-1g | Orcein | 產品資料 | 1 g | |
| GC307037-1g | Carmine | 產品資料 | 1 g | |
| GC307039-10g | Mordant Blue 29 | 產品資料 | 10 g | |
| GC307042-5g | Oil Red O | 產品資料 | 5 g | |
| GC307045-25g | Rhodamine B | 產品資料 | 25 g | |
|
Vitamins
|
GC301015-25g | Vitamin C | 產品資料 | 25 g |
| GC303001 | Folic acid | 產品資料 | 5 g | |
| GC303004 | Ribofavin | 產品資料 | 25 g | |
| GC303005-5g | VitaminB6 | 產品資料 | 5 g | |
| GC303006-25g | VitaminB3 | 產品資料 | 25 g | |
| GC303008 | VitaminB12 | 產品資料 | 1 g | |
| GC303011-25mg | NADPH-Na4 | 產品資料 | 25 mg | |
| GC303013-250mg | β-nicotinamide adenine dinucleotide NAD | 產品資料 | 250 mg | |
| GC303014-250mg | NADH-Na2 | 產品資料 | 250 mg | |
|
Antibiotics
|
GC301002 | Streptozocin STZ | 產品資料 | 1 g |
| GC301002-100mg | Streptozocin STZ | 產品資料 | 100 mg | |
| GC301003 | Penicillin G Sodium salt | 產品資料 | 1 g | |
| GC301004 | Ampicillin | 產品資料 | 1 g | |
| GC301005 | Streptomycin sulfate | 產品資料 | 5 g | |
| GC301008 | Kanamycin | 產品資料 | 1 g | |
| GC301009 | Kanamycin sulfate | 產品資料 | 5 g | |
| GC301009-1g | Hygromycin B | 產品資料 | 1 g | |
| GC301011 | Geneticin G-418 | 產品資料 | 1 g | |
| GC301013 | Chloramphenicol | 產品資料 | 5 g | |
| GC301013-10mg | Pseudomonic acid | 產品資料 | 10 mg | |
| GC301014-5g | Norfloxacin | 產品資料 | 5 g | |
| GC301016 | Cisplatin | 產品資料 | 100 mg | |
| GC301017-100mg | Sirolimus | 產品資料 | 100 mg | |
|
Heterocyclic compounds
|
GC207001 | Imidazole | 產品資料 | 10 g |
| GC207002 | PMSF | 產品資料 | 5 g | |
| GC207003 | Freund’s Adjuvant Complete | 產品資料 | 10 mL | |
| GC207004 | Freund’s Adjuvant Incomplete | 產品資料 | 10 mL | |
|
Salt
|
GC201001 | Guanidine HCl | 產品資料 | 500 g |
| GC201002 | Guanidine thiocyanate | 產品資料 | 500 g | |
|
Sugar Alcohols
|
GC205001 | L(+)-Lactic acid | 產品資料 | 100 g |
| GC205002 | Pyruvic acid sodium salt | 產品資料 | 25 g | |
| GC205003 | D-Glucose, anhydrous | 產品資料 | 500 g | |
| GC205004-10g | D(+)-Trehalose dihydrate | 產品資料 | 10 g | |
| GC205005 | D(+)- Galactose | 產品資料 | 100 g | |
| GC205006-10g | D-(-)Arabinose | 產品資料 | 10 g | |
| GC205007-1g | DTE | 產品資料 | 1 g | |
| GC205008 | Glycerol | 產品資料 | 100 mL | |
| GC205009 | LPS | 產品資料 | 10 mg | |
| GC205010 | DTT | 產品資料 | 1 g | |
| GC205011 | IPTG | 產品資料 | 1 g | |
| GC205013 | Agarose | 產品資料 | 100 g | |
| GC205014 | Sucrose | 產品資料 | 500 g | |
| GC205015 | D-Sorbitol | 產品資料 | 500 g | |
|
Solvent
|
GC203001 | TEMED | 產品資料 | 100 mL |
| GC203002 | DMSO(Dimethyl sulfoxide) | 產品資料 | 100 mL | |
| GC203003 | Urea | 產品資料 | 500 g | |
| GC203005 | DMSO cellular level | 產品資料 | 10 mL | |
|
Complex
|
GC202001 | EDTA | 產品資料 | 500 g |
| GC202002 | EDTA -Na2 | 產品資料 | 500 g | |
|
Buffer salt
|
GC208001 | Tris (Hydroxymethyl) Aminomethane | 產品資料 | 500 g |
| GC208003 | RIS-HCL | 產品資料 | 500 g | |
| GC208004-100g | BIS-TRIS | 產品資料 | 100 g | |
| GC208006 | N-Tris[Hydroxymethyl]Methylglycine | 產品資料 | 100 g | |
| GC208007 | 4-propanesulfonyl morpholine | 產品資料 | 100 g | |
| GC208008-25g | HEPPS | 產品資料 | 25 g | |
| GC208009 | HEPES, Free acid | 產品資料 | 100 g | |
| GC208012-1g | TCEP·HCL | 產品資料 | 1 g | |
|
Surfactant
|
GC204001 | Tween-80 | 產品資料 | 100 mL |
| GC204002 | Tween-20 | 產品資料 | 100 mL | |
| GC204003 | Triton X-100 | 產品資料 | 100 mL | |
| GC204004 | Sodium dodecylsulfate | 產品資料 | 500 g | |
| GC204005 | Sodium dodecylsulfate | 產品資料 | 100 g | |
| GC204006 | Nonidet TM P40 Substitute NP-40 | 產品資料 | 100 g | |
| GC204007 | Deoxycholic acid， sodium salt | 產品資料 | 25 g | |
| GC204009-100ml | APES | 產品資料 | 100 mL | |
|
Amine organics
|
GC206002 | Acrylamide | 產品資料 | 500 g |
| GC206003 | N’-MBA | 產品資料 | 100 g | |
|
Polymer
|
GC209002-250g | PEG 8000 | 產品資料 | 250 g |
| GC209003-250g | PEG 6000 | 產品資料 | 250 g | |
| GC209004-100g | PEG 3350 | 產品資料 | 100 g | |
| GC209005-500g | Poly(ethylene glycol)2000 | 產品資料 | 500 g | |
| GC209006-250g | Poly(ethylene glycol) 20000 | 產品資料 | 250 g | |
|
Inorganic salt
|
GC106001 | Orthoboric acid | 產品資料 | 500 g |
| GC104004 | Sodium bicarbonate | 產品資料 | 500 g | |
| GC108003 | Citric acid monohydrate | 產品資料 | 100 g | |
| GC108004 | Sodium citrate dihydrate | 產品資料 | 100 g | |
| GC102001 | Calcium chloride | 產品資料 | 100 g | |
| GC102002 | Magnesium chloride hexahydrate | 產品資料 | 100 g | |
| GC102003 | Potassium chloride | 產品資料 | 500 g | |
| GC102004 | Sodium chloride | 產品資料 | 500 g | |
| GC101004 | Magnesium Sulfate | 產品資料 | 100 g | |
| GC101013 | Ammonium persulfate | 產品資料 | 25 g | |
| GC105001 | Sodium phosphate monobasic | 產品資料 | 500 g | |
| GC105002 | Sodium Phosphate, Dibasic | 產品資料 | 500 g | |
| GC105003 | Potassium Phosphate Monobasic | 產品資料 | 500 g | |
| GC107001 | Sodium hydroxide | 產品資料 | 100 g | |
| GC306001 | Lodixanol | 產品資料 | 5 g | |
| GC310001-100g | skimmed milk powder | 產品資料 | 100 g |