β-葡萄糖苷酶測定試劑盒| beta-Glucosidase Assay Kit (ab272521)
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β-葡萄糖苷酶測定試劑盒beta-Glucosidase Assay Kit | ABCAM貨號ab272521
產品描述:
β-葡萄糖苷酶檢測試劑盒 (Beta-Glucosidase Assay Kit, ab272521) 是一種簡單、直接且自動化的程序，用於測量 β-葡萄糖苷酶(β-glucosidase)。 β 葡萄糖苷酶檢測試劑盒 (beta Glucosidase Assay Kit, ab272521) 旨在直接測量生物樣品中的 β-葡萄糖苷酶(β-glucosidase)活性，無需預處理。改進的方法利用對硝基苯基-β-D-吡喃葡萄糖苷，它被β-葡萄糖苷酶特異性水解成黃色產物（最大吸光度在405nm處）。反應速率與酶活性成正比。高靈敏度和寬線性範圍。使用 20 μL 樣品。檢測限為 2 U/L，線性高達 250 U/L。程序均質且簡單。簡單的“混合和測量”程序可以在 20 分鐘內對 β-葡萄糖苷酶(β-glucosidase)活性進行可靠的定量。堅固耐用且適合 HTS。所有試劑均與高通量液體處理儀器兼容。
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example of beta-Glucosidase standard curve.
Example of beta-Glucosidase reaction progress curves.
Storage instructions
| Components | 100 tests |
|---|---|
| β-NPG Substrate | 1 x 1ml |
| Assay Buffer | 1 x 24ml |
| Calibrator | 1 x 10ml |
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Product name
beta-Glucosidase Assay Kit -
Detection method
Colorimetric -
Assay type
Quantitative -
Range
2 mU/ml – 250 mU/ml -
Product overview
Beta-Glucosidase Assay Kit (ab272521) is a simple, direct and automation-ready procedure for measuring β-glucosidase activity. beta-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L.
Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of β-glucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate