檸檬酸鹽( Citrate)檢測試劑盒(BioAssay Systems’ Citrate Assay Kit) BioAssay Systems 貨號ECIT-100

可檢測線性檢測範圍：檸檬酸鹽為 4 至 400 µM含量

CITRATE 是檸檬酸循環(citric acid cycle )的中間體，參與脂肪酸合成(fatty acid synthesis)。 BioAssay Systems 的檸檬酸鹽檢測試劑盒(BioAssay Systems’ Citrate Assay Kit)提供了一種簡單且自動化的程序來測量檸檬酸鹽濃度(citrate concentration)。檸檬酸鹽( Citrate)轉化為丙酮酸鹽( pyruvate)，然後丙酮酸鹽被氧化，染料轉化為有色和螢光形式( fluorescent form)。 570 nm 處的顏色強度或 λex/em = 530/585 nm 處的螢光強度與樣品中檸檬酸鹽的濃度成正比。

主要特點 (KEY FEATURES)

快速、靈敏。線性檢測範圍：用於比色測定的檸檬酸鹽為 4 至 400 µM，用於螢光測定的檸檬酸鹽為 0.5 至 40 µM。方便、高通量。均質(Homogeneous)“混合-孵育測量(mix-incubatemeasure)”型測定。可在 HTS 液體處理系統(HTS liquid handling systems)上輕鬆實現自動化，每天處理數千個樣品。應用 生物樣品（例如血漿、血清、尿液、組織和培養基）中的檸檬酸鹽(Citrate)測定。

注意事項Sample Preparation:

Tissue or cell samples (2 x10⁶) can be homogenized in 100 μL PBS. Centrifuge at 14,000 rpm for 5 min. Use clear supernatant for assay. If planning to measure citrate in culture media, avoid media with high pyruvate concentrations (e.g. DMEM, L-15, F12, etc.).

Serum and plasma samples should be deproteinated using a 10 kDa spin filter (e.g. Amicon Ultra-0.5). Alternatively, untreated serum and plasma can measured directly if an internal standard is used.

Urine samples should be diluted at least 5-fold and an internal standard should be used.

Application

• For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.

Key Features

• Fast and sensitive. Linear detection range: 4 to 400 µM citrate for colorimetric assays and 0.5 to 40 µM for fluorimetric assays.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD570nm, or FL530/585nm

Samples

• Serum, plasma, agriculture etc

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• OD, FL: 4, 0.5 µM

Shelf Life

• 6 months

More Details

• CITRATE is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. BioAssay Systems’ Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample.

BioAssay Systems 品牌介紹

BioAssay Systems 公司研製開發和銷售創新(innovative)高通量的檢測試劑組(high-throughput assay solutions)，以滿足不斷增加的需求的生命科學行業(life sciences industry)。BioAssay Systems 專注於安全，無放射性等格式的吸收光，螢光和發光檢測技術。BioAssay Systems 在現代藥物發現方面擁有 50 多年的豐富經驗。在過去的 18 年中，開發 200 多種用於藥物發現和生命科學研究的檢測試劑盒。提供的服務和簡單、使用方便且性能卓越的產品而自豪。

BioAssay Systems檢測試劑盒及試劑

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