草酸鹽檢測試劑盒(BioAssay Systems’ oxalate assay kit ) | BioAssay Systems 貨號EOXA-100
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草酸鹽檢測試劑盒(BioAssay Systems’ oxalate assay kit ) BioAssay Systems 貨號EOXA-100
可檢測線性檢測範圍：20 至 1500 µM 草酸鹽含量
樣品預處理更快、更容易。高通量適應性強
草酸鹽(Oxalate)或草酸(Oxalic Acid )是真核生物(eukaryotes)克雷布循環(Kreb’s Cycle )和其他微生物(microorganisms)乙醛酸循環( glyoxylate cycle)的代謝分解產物。它可以在人類和其他哺乳動物的尿液中找到。草酸鹽濃度( Oxalate concentration )可用作腎功能的衡量標準，其中高水平的草酸鹽是腎結石(kidney stones)的指標，腎結石(kidney stones)主要由不溶性草酸鈣鹽組成。作為腎結石的標誌物，測量草酸鹽比測量鈣更準確，因為即使在正常尿液中，鈣也會以高濃度排出。用於測量草酸濃度的簡單、直接和高通量的測定方法具有廣泛的應用。 BioAssay Systems 的草酸鹽檢測試劑盒(BioAssay Systems’ oxalate assay kit )使用單一工作試劑，將草酸鹽氧化酶反應(oxalate oxidase reaction)和顯色反應結合在一個步驟中。反應產物在 595 nm 處顏色強度的變化與樣品中的草酸鹽成正比。
主要特點 (KEY FEATURES)
靈敏、準確。使用少至 10 µL 的樣品。 96 孔板中孵育 10 分鐘的線性檢測範圍：20 至 1500 µM 草酸鹽。快捷方便。與其它檢測試劑盒中使用活性炭(activated carbon)相比，樣品預處理更快、更容易。高通量適應性強。該程序包括添加單一工作試劑並在室溫下孵育 10 分鐘。可以自動化每天處理數千個樣品。應用 直接測定：尿液、動物和植物組織樣品中的草酸鹽濃度。可應用於藥物發現/藥理學：藥物對草酸鹽濃度、代謝和排泄的影響。
注意事項:
程序 樣品可在採集後立即進行分析，或分裝在 4°C 或 –20°C 下保存 7 天。為了長期保存，每 1 mL 樣品添加 20 µL 6N HCl ，並保存在 -80°C 下。測定前，用 20 µL 6N NaOH 中和樣品。通過乘以 n = 1.04 來校正稀釋因子。避免反覆凍融循環。如果存在顆粒，則離心樣品並使用澄清的上清液進行測定。將所有組件平衡至室溫。實驗過程中，將解凍的酶保存在冰箱或冰上。
Application
- For quantitative determination of oxalate (oxalic acid) in urine and other samples.
Key Features
- Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 10 minute incubation: 20 to 1500 µM oxalate.
- Fast and convenient. Sample pre-treatment is faster and easier than using activated carbon in competitor’s assay kits.
- High-throughput adaptable. The procedure involves addition of a single working reagent and incubation for 10 min at room temperature. Can be automated for processing thousands of samples per day.
Method
- OD595nm
Samples
- urine, animal and plant tissue samples
Species
- All
Procedure
- 10 min
Size
- 100 tests
Detection Limit
- 20 µM
Shelf Life
- 6 months
More Details
- Oxalate or Oxalic Acid is a metabolic breakdown product of the Kreb’s Cycle in eukaryotes, and the glyoxylate cycle in other microorganisms. It can be found in the urine of humans and other mammals. Oxalate concentration can be used as a measure of kidney function where a high level of oxalate is an indicator for kidney stones which are primarily made of the insoluble salt calcium oxalate. Measuring oxalate is more accurate than measuring calcium as a marker for kidney stones because calcium is excreted at high concentrations even in normal urine. Simple and high-throughput assays for measuring oxalate concentration find wide applications. BioAssay Systems’ oxalate assay kit uses a single Working Reagent that combines the oxalate oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 595 nm is directly proportional to oxalate in the sample.
BioAssay Systems 品牌介紹
BioAssay Systems 公司研製開發和銷售創新(innovative)高通量的檢測試劑組(high-throughput assay solutions)，以滿足不斷增加的需求的生命科學行業(life sciences industry)。BioAssay Systems 專注於安全，無放射性等格式的吸收光，螢光和發光檢測技術。BioAssay Systems 在現代藥物發現方面擁有 50 多年的豐富經驗。在過去的 18 年中，開發 200 多種用於藥物發現和生命科學研究的檢測試劑盒。提供的服務和簡單、使用方便且性能卓越的產品而自豪。
BioAssay Systems檢測試劑盒及試劑
- Agriculture & Environment
- Blood & Urine Chemistry
- Cations & Anions
- Diabetes & Obesity
- Enzyme Activity
- Food & Beverage Analysis
- HTS Reagents & Kits
- Inhibitor HTS Kits
- Metabolism
- Oncology
- Other Reagents
- Oxidative Stress
- Quick Test Strips
- Signal Transduction
Hong, S., et al. (2020). Ginsenoside compound-Mc1 attenuates oxidative stress and apoptosis in cardiomyocytes through an AMP-activated protein kinase-dependent mechanism.Journal of Ginseng Research.44(4): 664-671. Assay: Collagen in mouse heart tissue.
