外泌體(Exosomes)分離 | 細胞外囊泡 (Extracellular Vesicle, EV)純化
| 代理廠牌： |  |
| 原廠連結： | |
| 相關下載： |
外泌體(Exosomes)分離- 細胞外囊泡 (Extracellular Vesicle, EV)純化
外泌體(Exosomes )是小的核內體(endosome)衍生的50-120 nm脂質納米顆粒( lipid nanoparticles)，在大多數活細胞中通過胞吐作用(exocytosis)積極分泌。外泌體(Exosomes )具有多效性生理和病理功能，在癌症(cancer)、傳染病(infectious)和神經退行性疾病(neurodegenerative diseases)等多種病理狀況中發揮重要作用。 AMSBIO 提供多種用於外泌體純化(exosome purification)、表徵(characterization)和高質量試劑和試劑盒，應用於外泌體研究。
ExoPure 是一種從生物流體(biofluids )和細胞培養物上清液(cell culture supernatants)中分離外泌體的快速簡便的方法。方法基於化學沉澱(chemical precipitation)。樣品在冰中的ExoPure solution溶液反應，然後通過離心(centrifugation)分離外泌體並溶解( solubilizedin)在PBS 1X 或去離子水。程序易於執行，不耗時（約 1小時），不需要超速離心，也不需要昂貴的實驗室設備。分離的外泌體適用於廣泛的分析，例如 NTA、蛋白質分析不同的技術（Western Blotting、ELISA、FACS）、核酸提取(nucleic acids extraction)和分析mRNA 或 miRNA 標記物。
外泌體分離 (Exosome Isolation)
外泌體分離
AMSBIO開發和優化了用於快速、可擴展和可重複的細胞外囊泡 (Extracellular Vesicle, EV) 純化的方法和工具，並解決了 (Extracellular Vesicle, EV) 異質性和用於生物標誌物發現的高通量解決方案提供快速、經濟高效的解決方案。儘管超速離心仍被廣泛使用，但它往往會改變囊泡的形狀(vesicle shape)和功能。這種耗時的方法和對昂貴設備的需求意味著它不再被視為 (Extracellular Vesicle, EV) 分離的黃金標準。
Our products however; offer fast, cost-effective solutions for all your EV isolation needs.
ExoPure：化學分離(Chemical isolation)
ExoPure：化學分離是一種基於化學沉澱(chemical precipitation based)的方法，可快速方便地從生物體液、細胞培養上清液和植物提取物中分離細胞外囊泡 (Extracellular Vesicle, EV)。該方法基於化學沉澱(chemical precipitation)。樣品與 ExoPure 溶液一起在冰上孵育，以便細胞外囊泡 (Extracellular Vesicle, EV) 在離心後沉澱，然後離心分離外泌體(exosome)並溶解在 PBS 1X 或去離子水中。獲得的顆粒可以重新懸浮在 PBS 1X 或去離子水中。程序易於執行，不耗時（約 1 小時），不需要超速離心或昂貴的實驗室設備。這些分離的囊泡適用於適用於多種分析，例如 NTA、使用不同技術（蛋白質印跡、ELISA、FACS）進行蛋白質分析、核酸萃取(nucleic acids extraction)以及 mRNA 或 miRNA 標記物分析。
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| ExoPure for exosome isolation from Cell Media, K1237-25 |  |
25 ml (25 react) | View |
| ExoPure for exosome isolation from Plasma and serum, K1238-5 | |
5 ml | View |
| ExoPure for exosome isolation from Urine, K1240-25 | |
30 ml (25 react) | View |
Tangential Flow Filters for EV concentration and purification
用於 EV 濃縮和純化的切向流過濾器(Tangential flow filtration, TFF)
切向流過濾(Tangential flow filtration, TFF)是一種快速高效的方法，工業應用中常用於生物分子的分離純化，可成功應用於細胞外囊泡 (Extracellular Vesicle, EV) 的分離。我們提供三種不同類型的過濾器，分別適用於細胞外囊泡 (Extracellular Vesicle, EV) 純化、細胞外囊泡 (Extracellular Vesicle, EV) 濃縮和基於 細胞外囊泡 (Extracellular Vesicle, EV) 大小的分離。
TFF-Simple：用於 細胞外囊泡 (Extracellular Vesicle, EV) 濃縮和透析的過濾器
TFF-Simple：用於 EV 濃縮和透析的過濾器
TFF-Simple 是一種由聚砜(polysulfone)製成的中空纖維(hollow fibers )濾芯(filter cartridge )，可在細胞外囊泡 (Extracellular Vesicle, EV) 純化之前從稀釋基質(diluted matrices)（細胞條件培養基、尿液等）中濃縮顆粒並去除小蛋白質和分子。
Applications
- Concentration of diluted fluid as cell media or urine prior to EV isolation
- Easy removal of small molecules and ions from the EV preparation
- EV dialysis and buffer exchange
- High efficiency of EV isolation if coupled with SEC columns
Advantages
- Washable
- Reusable multiple times. Easy to use
- Fast concentration of EV containing matrices
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| TFF-Simple – tangential flow filtration concentrator | |
1 concentrator | View |
| TFF-Simple – tangential flow filtration concentrator | |
5 concentrators | View |
TFF-Micro：用於大>150 nm 細胞外囊泡 (Extracellular Vesicle, EV)分離和純化的過濾器
TFF-Micro：用於>150 nm細胞外囊泡 (Extracellular Vesicle, EV) 分離和純化的過濾器
TFF-Micro 是一種能夠按大小分離大微泡 (MV) 的過濾器，避免了在 10000g 下離心分離，這通常會導致部分小 細胞外囊泡 (Extracellular Vesicle, EV) 的損失。 TFF-Micro 保留大於 150-200 nm 的囊泡，而小的 細胞外囊泡 (Extracellular Vesicle, EV) 和循環分子則通過滲透液。保留的 MV 可以在 PBS 緩衝液中用注射器回收，無需額外的純化步驟。
Applications
- Separation, concentration and recovery of large EVs (>150 nm)
- Large EV isolation from cell media, biofluids, plant extracts
- Dialysis and desalting of large EVs
- Suitable for large EV isolation from 5 ml of fluid
Advantages
- Washable
- Reusable multiple times
- Sterile
- Fast separation of EVs bigger than 150 nm
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| TFF-Micro- tangential flow filter for large EV separation and recovery | |
1 filter | View |
TFF-Exo：快速 細胞外囊泡 (Extracellular Vesicle, EV) 淨化
TFF-Exo：快速細胞外囊泡 (Extracellular Vesicle, EV) 淨化
TFF-Exo 是新一代過濾器，可對細胞外囊泡 (Extracellular Vesicle, EV) 進行快速、可重複和可擴展的純化，可在實驗室工作台上用於純化少量樣品（最少 5 毫升）或與機械系統連接以純化更大體積的樣品。TFF-Exo 是一種濾芯(filter cartridge)，由聚醚砜中空纖維( polyethersulfone hollow fibers)製成，孔徑為 50 nm（截止 300 kDa）。該過濾器允許純化細胞外囊泡 (Extracellular Vesicle, EV) 和 > 50 nm 的顆粒。
Applications
- Separation, purification and concentration of EVs from conditioned media, biofluids, plant extracts
- Buffer exchange and removal of unbound dye
- Depletion of FBS from bovine EVs
Advantages
- Washable
- Reusable multiple times
- Sterile
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| TFF-Exo – tangential flow filter for EV purification, large | |
1 filter | View |
| TFF-Exo – tangential flow filter for EV purification, small | |
1 filter | View |
ExoSpin：超濾濃縮器 (Ultrafiltration concentrator)
ExoSpin：超濾濃縮器(Ultrafiltration concentrator)
ExoSpin 是一種 2.5 ml 不粘超濾 (non-stick ultrafiltration , UF) 濃縮器，可在濃縮或緩衝液交換步驟中增強細胞外囊泡 (Extracellular Vesicle, EV) 的回收。超濾以與離心力相反的方向工作，提供更高的顆粒回收率。與 V 形濃縮器相比，低蛋白結合膜（聚醚砜）減少了 細胞外囊泡 (Extracellular Vesicle, EV) 損失，細胞外囊泡 (Extracellular Vesicle, EV) 旋轉器的反向設計確保過濾器不會堵塞。
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| ExoSpin: Ultrafiltration concentrator, MWCO 100 kDa, 24 pcs. | |
24 concentrators | View |
| ExoSpin: Ultrafiltration concentrator, MWCO 100 kDa, 48 pcs. | |
48 concentrators | View |
ExoUltra：體積排阻層析(Size exclusion chromatography)
ExoUltra：體積排阻層析(Size exclusion chromatography)
尺寸排阻色譜 (Size Exclusion Chromatography, SEC) 是一種從不同液體中分離和純化細胞外囊泡 (Extracellular Vesicle, EV)的有效方法，不會影響囊泡的原始形狀和功能。我們的 ExoUltra SEC 色譜柱系列允許從小體積（100 微升）到大體積（最多 20 毫升）液體進行細胞外囊泡 (Extracellular Vesicle, EV) 純化。使用 ExoUltra 柱進行細胞外囊泡 (Extracellular Vesicle, EV) 純化大約需要 15 分鐘，可以集成到任何實驗室工作流程中。
Characteristics
- New gel matrix for EV purity improvement
- Purification up to 20 ml volume of fluid
Advantages
- Easy and fast protocol (turnaround time approximately 15 minutes). Isolate EVs from small sample volumes
- Reusable up to 5 times
- Long term stability at 4°C
Applications
- Extracellular vesicles isolation from cell media, biofluids and plant extracts
- Purification of EVs from contaminants
- Dye excess removal post EV labelling process
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| ExoUltra PLUS: Size exclusion chromatography columns and TFF concentrator | – | 5 columns + 1 TFF concentrator | View |
| ExoUltra PLUS: Size exclusion chromatography columns, TFF-Simple, TFF-Micro | – | 5 columns + 2 TFF concentrator | View |
| ExoUltra-max PLUS: Size exclusion chromatography column and TFF concentrator | – | 1 column + 1 TFF concentrator | View |
| ExoUltra-max: Size exclusion chromatography column | |
3 columns | View |
| ExoUltra-max: Size exclusion chromatography column | |
6 columns | View |
| ExoUltra-min: Size exclusion chromatography column | |
10 columns | View |
| ExoUltra-min: Size exclusion chromatography column | |
20 columns | View |
| ExoUltra: Size exclusion chromatography column | |
10 columns | View |
| ExoUltra: Size exclusion chromatography column | |
5 columns | View |
ExoUltra：免疫親和分離(Immunoaffinity isolation)
Understanding and addressing extracellular vesicle heterogeneity has become essential for the EV field, especially because of the different roles that EVs have in pathological processes such as cancer, infection, and neurodegenerative diseases.
Our pre-coated ELISA immunoplates and our latex or magnetic immunobeads are perfect for the capture and enrichment of total or specific EV-subpopulations.
ExoUltra：免疫親和分離(Immunoaffinity isolation)
理解和解決細胞外囊泡異質性對於細胞外囊泡 (Extracellular Vesicle, EV) 領域至關重要，特別是因為 細胞外囊泡 (Extracellular Vesicle, EV) 在癌症、感染和神經退行性疾病等病理過程中具有不同的作用。預包被 ELISA 免疫板和乳膠或磁性免疫珠非常適合捕獲和富集全部或特定 細胞外囊泡 (Extracellular Vesicle, EV) 亞群。
Applications
- Multiple profiling of EV markers from both a single sample or screening a large number of samples
- EV capture and quantification from human biofluids (plasma, serum, urine, saliva)
- Immunocaptured EVs are suitable for downstream processes such as nucleic acid extraction
Advantages
- Ready to use.
- Long term storage (24 months).
- No EV pre-purification required
- Small sample amounts required (100 μl per well).
- Flexibility and ease when designing a multiplexing assay.
- Open platform for customized coating solutions.
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| ExoUltra Immunoplate for capture CD63 positive Exosomes (Concentrated Cell culture supernatants) | |
1 plate | View |
| ExoUltra Immunoplate for capture EphrinB positive exosomes | |
1 plate | View |
| ExoUltra Immunoplate for capture L1CAM positive exosomes | |
1 plate | View |
| ExoUltra Immunoplate for capture PLP1 positive Exosomes | |
1 plate | View |
| ExoUltra Immunoplate for capture TM9SF4 positive exosomes | |
1 plate | View |
| ExoUltra Immunoplate for capture of CD9 positive Exosomes (Human Serum) | |
1 plate | View |
| Immunobeads for Mouse exosome capture (CD9 Positive) | |
10 reactions | View |
| Immunobeads for capture CD63 positive Exosomes | |
10 reactions | View |
| Immunobeads for capture TM9SF4 positive exosomes | |
10 reactions | View |
| Immunobeads for capture of CD9 positive Exosomes | |
10 reactions | View |