Mouse Anti-Chicken CD3-UNLB (CT-3)抗體 | Southernbiotech貨號8200-01
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Mouse Anti-Chicken CD3-UNLB (CT-3)抗體 | 貨號8200-01
Purified Anti-Mouse H-2Kd/H-2Dd antibody for use in flow cytometry, immunocytochemistry, electron microscopy, immunoprecipitation, CMCD, blocking, and adhesion assays.
細胞免疫專家極力推薦SouthernBiotech高品質抗體
純化的抗雞 CD3 抗體，用於流式細胞術(flow cytometry)、免疫組織化學(immunohistochemistry)、免疫沉澱(immunoprecipitation)和刺激試驗( stimulation assays)。CD3 是 T 細胞受體相關 CD3 複合物的成員(T cell receptor-associated CD3 complex)。單克隆抗體 CT-3 可識別雞 T 細胞上至少三種 Mr 20、19 和 17 kDa（其中兩種是 N-糖基化）多肽(polypeptides)的複合物。該抗體還從毛地黃皂苷溶解(digitonin solubilized )的 T 細胞裂解物中共沉澱(coprecipitates a polypeptide )出 90 kDa 的多肽，該多肽可減少為 Mr 50 和 40 kDa 的兩種多肽。Mouse Anti-Chicken CD3-UNLB (CT-3)抗體，免疫原為Chicken thymocytes and Ig– blood mononuclear cells。可應用於Flow Cytometry – Quality tested、Immunohistochemistry-Frozen Sections 、Immunohistochemistry-Paraffin Sections 、Immunoprecipitation、Stimulation等試劑。
| Clone | CT-3 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-UNLB (15H6) |
| Specificity | Chicken/Pigeon CD3 |
| Alternative Names | T3/TCR complex |
| Description | CD3 is a member of the T cell receptor-associated CD3 complex. The monoclonal antibody CT-3 recognizes a complex of at least three polypeptides of Mr 20, 19, and 17 kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90 kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50 and 40 kDa. |
| Immunogen | Chicken thymocytes and Ig– blood mononuclear cells |
| Conjugate | UNLB (Unconjugated) |
| Buffer Formulation | Borate buffered saline, pH 8.2 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications | Flow Cytometry – Quality tested 1,5,10-18 Immunohistochemistry-Frozen Sections – Reported in literature 2-8 Immunohistochemistry-Paraffin Sections – Reported in literature 9 Immunoprecipitation – Reported in literature 1 Stimulation – Reported in literature 1 |
| RRID Number | AB_2796417 |
| Gene ID | 396062 (Chicken) 102092091 (Pigeon) |
| Gene ID Symbol | CD3E (Chicken) CD3E (Pigeon) |
| UniProt ID | Q98910 (Chicken) |
| UniProt Name | CD3E_CHICK (Chicken) |
美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全，包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質（FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）各種螢光標誌、另具酵素（HRP、AP）、或未標誌及Low Endotoxin/Azide Free（LE/AF）等標定。
Isotype control 分型對照組 |分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |
