透明質酸 (HA)檢測試劑盒 (酶聯免疫吸附試驗法) | CLOUD CLONE貨號CEA182Ge
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特點
透明質酸 (HA)檢測試劑盒 (酶聯免疫吸附試驗法) | CLOUD CLONE貨號CEA182Ge
ELISA Kit for Hyaluronic Acid (HA)
產品介紹: 透明質酸 (HA)檢測試劑盒 (酶聯免疫吸附試驗法) ，偵測的靈敏度4.94-400ng/mL、最低靈敏度1.71ng/mL。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。該檢測方法對透明質酸 (HA) 具有高靈敏度和出色的特異性。未觀察到透明質酸 (HA) 和類似物之間的顯著交叉反應或干擾。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法)，專門生產研究用檢測試劑的生技公司，於美國研發和生產，產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein；ELISA產品系列超過6000種以上，物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。Uncoated ELISA型式；包括Capture Antibody, Detection Antibody、Standard、Streptavidin-HRP、TMB Substrate, 96-well Plate。
Specificity
This assay has high sensitivity and excellent specificity for detection of Hyaluronic Acid (HA).
No significant cross-reactivity or interference between Hyaluronic Acid (HA) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Hyaluronic Acid (HA) and the recovery rates were calculated by comparing the measured value to the expected amount of Hyaluronic Acid (HA) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 80-93 | 85 |
| heparin plasma(n=5) | 92-101 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyaluronic Acid (HA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyaluronic Acid (HA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hyaluronic Acid (HA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-101% | 95-104% | 79-88% | 84-98% |
| EDTA plasma(n=5) | 79-97% | 78-104% | 87-105% | 89-103% |
| heparin plasma(n=5) | 96-105% | 89-97% | 94-105% | 97-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
透明質酸 (HA) 檢測試劑盒 (酶聯免疫吸附試驗法)套組內容
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
透明質酸 (HA) 檢測試劑盒簡單流程
- Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
透明質酸 (HA) 檢測試劑盒相關之抗體 | 蛋白質 | ELISA | CLIA產品
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CPA182Ge11 | BSA Conjugated Hyaluronic Acid (HA) | Immunogen; SDS-PAGE; WB. |
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