碘化丙啶(Propidium iodide, PI) 核酸染劑 | A red-fluorescent DNA stain CAS# 25535-16-4 APExBIO貨號B7758
碘化丙啶(Propidium iodide, PI) 核酸染劑 | A red-fluorescent DNA stain CAS# 25535-16-4 APExBIO貨號B7758
A red-fluorescent DNA stain
產品介紹
碘化丙啶(Propidium iodide)是一種細胞膜不可滲透(cell-membrane impermeable)的染料，經常用於 DNA 染色，最大激發波長(excitation maximum)為 535 nm，最大發射波長(emission maximum)為 617 nm。雖然碘化丙啶(Propidium iodide)通常被排除在具有完整膜(intact membranes)的活細胞之外，但它可以輕鬆穿透死亡(dead)或受損細胞(damaged cells)，這使得它通常用於鑑定細胞群中的細胞活力(cell viability)或用作多色螢光技術(multicolor fluorescent techniques)中的複染劑。碘化丙啶(Propidium iodide)以每 4 ~ 5 個鹼基對一種染料的化學計量嵌入核酸，幾乎沒有序列偏好，然後通過螢光顯微鏡(microscopy)和流式細胞術(flow cytometry)檢測。碘化丙啶(Propidium iodide)已被證明是評估異質組織(heterogeneous tissues)（包括骨髓、淋巴結和胸腺）中某些細胞群凋亡(apoptosis)的有用工具。
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 668.39 |
| Cas No. | 25535-16-4 |
| Formula | C27H34I2N4 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥9.84 mg/mL in DMSO |
| Chemical Name | 3,8-diamino-5-(3-(diethyl(methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide |
| SDF | Download SDF |
| Canonical SMILES | NC1=CC2=[N+](C(C3=CC=CC=C3)=C(C=C4N)C(C=C4)=C2C=C1)CCC[N+](CC)(C)CC.[I-].[I-] |
| Shipping Condition | Ship with blue ice, or upon other requests. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon. |
References:
- Coder DM. Assessment of cell viability. Current Protocols in Cytometry, 2001, Chapter 9: Unit 9.2.
- Hezel M, Ebrahimi F, Koch M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron, 2012, 43(10): 1031-1038.
- Nicoletti I, Migliorati G, Pagliacci MC, et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. Journal of Immunological Methods, 1991, 139(2): 271-279.
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