胰島素 ELISA (INS)試劑盒 | Human Insulin (INS) ELISA Kit貨號KTE62671

Human Insulin (INS) ELISA Kit

產品描述：胰島素 ELISA (INS)試劑盒採用兩點夾心ELISA(two-site sandwich ELISA)定量樣品中的胰島素 (Insulin (INS)。對胰島素 (Insulin (INS)特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中，並且存在的任何胰島素 (Insulin (INS)都被固定的抗體結合。除去所有未結合的物質後，將HRP-綴合胰島素 (Insulin (INS)檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後，將色原溶液添加到孔中，並且根據在初始步驟中結合的胰島素 (Insulin (INS)的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 1 mU/L-16 mU/L, 最低靈敏度0.1 mU/L。可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

Human Insulin (INS) ELISA Kit employs a two-site sandwich ELISA to quantitate Insulin in samples. An antibody specific for Insulin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Insulin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human Insulin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Insulin bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product name	Human Insulin (INS) ELISA Kit
Reactivity	Human
Applications	ELISA
Applications notes	Human Insulin (INS) ELISA Kit employs a two-site sandwich ELISA to quantitate Insulin in samples. An antibody specific for Insulin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Insulin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human Insulin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Insulin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method	Colorimetric
SampleType	Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type	Sandwich ELISA
Calibration range	1 mU/L-16 mU/L
Limit of detection	0.1 mU/L
Usage notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions	The unopened kit should be stored at 2 – 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

使用注意事項

• 請勿混用不同試劑盒中的組分，或在試劑盒到期日期之後使用試劑。
• 打開之前，讓所有試劑至少加室溫30分鐘。
• 用試劑預清洗移液器吸頭，為每個樣品，標準液和試劑使用新鮮的移液器吸頭，以免污染。
• 未使用的孔必須在提供的密封袋中於4°C乾燥。
• 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
• 建議所有樣品和標準品一式兩份或一式三份進行測定。

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