溶酶體細胞內活性檢測試劑盒 | Lysosomal Intracellular Activity Assay Kit貨號 K448 (ABCAM ab234622)
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溶酶體細胞內活性檢測試劑盒 | Lysosomal Intracellular Activity Assay Kit貨號 K448 (ABCAM ab234622)
溶酶體(Lysosomes)是對各種細胞過程很重要的膜結合細胞器(membrane-bound organelles)。它們含有用於某些生物分子代謝的水解酶(hydrolytic enzymes)。細胞外貨物(extracellular cargo)（例如營養素毒素, nutrients toxins）與細胞膜(cell membrane)結合，隨後被轉運到膜結合的內體(membrane-bound endosomes)中，由溶酶體(lysosomes)進一步降解，而細胞內成分通過自噬(autophagy)轉運到溶酶體。溶酶體功能障礙與許多人類疾病有關，例如衰老(aging)和神經退行性疾病(neurodegenerative disease)。雖然溶酶體(lysosomes)的細胞內活性(intracellular activity)難以在活細胞中測量，但 BioVision 開發了一種專有的溶酶體特異性自猝滅底物(Lysosome-Specific Self-Quenched Substrate)，該底物具有低背景螢光、高信背景比且對 pH 不敏感。作為內吞貨物的底物可以被細胞吸收並在內溶酶體囊泡內(endo-lysosomal vesicle)降解。螢光信號從自猝滅底物中恢復。降解產生的螢光信號與細胞內溶酶體活性成正比，可以使用螢光顯微鏡和/或流式細胞儀進行測量。溶酶體細胞內活性檢測試劑盒(Lysosomal Intracellular Activity Assay Kit)（細胞）包括 Cytochalasin D，一種可滲透細胞的內吞作用抑制劑，用作實驗對照。這種易於使用的非放射性試劑盒可以對培養細胞中的去淬滅底物進行成像和準確測量。
| abID | ab234622 |
|---|---|
| Cat # +Size | K448-50 |
| Size | 50 assays |
| Kit Summary | • Detection method- Flow cytometry and Fluorescence Microscopy • Species reactivity- Mammalian • Applications – Measurement of Intracellular lysosomal activity – Screening compounds with anti-lysosomal activity |
| Detection Method | Flow Cytometry (FL1)/ Microscopy (Ex: 488 nm) |
| Species Reactivity | Mammalian |
| Applications | • Measurement of lysosomal intracellular activity. • Elucidation of the mechanisms of endocytic pathway in living cells. • Screening compounds with anti-lysosomal intracellular activity |
| Features & Benefits | Measurement of lysosomal intracellular activity. |
| Kit Components | • Assay Buffer (50X) • Self-Quenched Substrate • Cytochalasin D (100X) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
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