Anti-PDL1 抗體 |absoluteantibody 貨號 Ab00373-1.32
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抗體介紹: Anti-PDL1 抗體對murine PDL1.的結合特異性 (Specificity)。此抗體可應用於ELISA, FC, IF等實驗。
Flow-cytometry using anti-PD-L1 antibody YDC 127.1.1 (Ab00373) Mouse spleenocytes
were stained with an isotype control (Ab00102-23.0, purple) or the rabbit-chimeric version of YDC 127.1.1 (Ab00373-23.0, grey) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.
Immunofluorescence staining of fixed mouse splenocytes with anti-PDL1 (CD274) antibody YDC 127.1.1 (Ab00373) Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of YDC 127.1.1 (Ab00373-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00373-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using anti-PDL1 antibody YDC 127.1.1 (Ab00373) Mouse lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of YDC 127.1.1 (Ab00373-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.
Alternative Name(s) of Target: : CD274; Programmed death-ligand 1; PD-L1; B7 Homolog 1; B7-H1; B7-H; PDCD1L1; PDCD1LG1
免疫原(Immunogen: Murine PDL1 UniProt Accession Number of Target Protein: Q9EP73
Size: 100 μg Purified antibody. Purification: Protein A affinity purified Supplied In: PBS with 0.02% Proclin 300. Storage Recommendation: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at – 20⁰C. Concentration: 1 mg/ml.
| 產品貨號 | Mouse target | 抗體clone | Original Antibody format |
| Ab01052 | 4-1BB (CD137) | LOB12.3 | Rat IgG1 |
| Ab01054 | 4-1BBL (CD137L) | AT113-2 | Rat IgG1 |
| Ab00881 | 4-1BBL (CD137L) | TKS-1 | Rat IgG2a |
| Ab01027 | BTLA | HMBT-6B2 |
Hamster IgG |
| Ab00670 | CD27 | LG.3A10 | Hamster IgG |
| Ab00895 | CD27 |
|
Rat IgG2a |
| Ab00286 | CD28 | E18 |
Mouse IgG2b |
| Ab00285 | CD28 | D665 |
Mouse IgG1 |
| Ab01109 | CD40 | 3/23 |
Rat IgG2a |
| Ab01087 | CD40L (CD154) | MR1 |
Hamster IgG |
| Ab00816 | CD70 | TAN 1-7 |
Rat IgG2a |
| Ab01056 | CD70 | TAN1-6 |
Rat IgG2a |
| Ab01028 | CD80 (B7-1) | RM80 | Rat IgG2a |
| Ab01029 | CD86 (B7-2) | PO.3 | Rat IgG2b |
| Ab01089 | CD153 | RM153 | Rat IgG2b |
| Ab01297 | CD96 | 6A6 | Rat IgG2a |
| Ab00894 | CTLA-4 | 9H10 | Hamster IgG2 |
| Ab01018 | CTLA-4 | 9D9 | Mouse IgG2b |
| Ab01088 | GAL-9 | RG9-35 | Rat IgG2a |
| Ab01060 | GITR | DTA-1 | Rat IgG2a |
| Ab00374 | GITR | YGITR 860.103.5 | Rat IgG2b |
| Ab00631 | GITR | YGITR765 | Rat IgG2b |
| Ab00630 | GITRL | YGL386 | Rat IgG1 |
| Ab01026 | HVEM | HMHV-1B18 | Hamster IgG |
| Ab00814 | ICOS | 7E.17G9 | Rat IgG2b |
| Ab00882 | ICOSL | HK5.3 | Rat IgG2a |
| Ab01295 | LAG3 | C9B7W | Rat IgG1 |
| Ab01295 | LAG3 | C9B7W | Rat IgG1 |
| Ab00110 | OX40 (CD134) | OX86 | Rat IgG1 |
| Ab00564 | OX40L | OX-89 | Rat IgG1 |
| Ab00813 | PD-1 | RMP1-14 | Rat IgG2a |
| Ab01017 | PD-1H (VISTA) | mam82 | Mouse IgG1 |
| Ab01016 | PD-1H (VISTA) | MH5A | Hamster IgG |
| Ab00373 | PD-L1 | YDC 127.1.1 | Rat IgG2a |
| Ab00883 | PD-L2 | TY25 | Rat IgG2a |
| Ab00875 | PDPN (MAP-Tag) | PMab-1 | Rat IgG2a |
| Ab00973 | TIGIT | 4D4 | Hamster IgG |
| Ab01258 | TIGIT | 1B4 | Mouse IgG1 |
| Ab01032 | TIM-1 | 3B3 | Rat IgG2a |
| Ab01110 | TIM-2 | RMT2-14 | Rat IgG2a |
| Ab01057 | TIM-3 | 2C12 | Rat IgG1 |
| Ab01058 | TIM-4 | 5G3 | Rat IgG1 |
| Ab01059 | TIM-4 | 3A1 | Rat IgG1 |