Amsbio台灣代理商-BRAND/AGENCY/代理品牌- Amsbio
Oris™ 細胞遷移檢測 – 膠原蛋白 | Oris™ Cell Migration Assay – Collagen I Coated貨號 CMACC1.101
細胞運動性(Cell motility)是活細胞的一個關鍵特徵。細胞遷移(Cell migration)和侵入(invasion)是參與正常發育(normal development)、免疫反應(immune response)和許多疾病(diseases)的關鍵過程。檢查(Examining)和理解(understanding)這些過程對於包括細胞生物學(cell biology)、癌症生物學(cancer biology)、發育生物學(developmental biology)和免疫學(immunology)在內的多個研究領域非常重要。
AMSBIO 提供使用獨特技術創建的基於細胞的遷移(cell-based migration)和細胞侵入檢測試劑盒(cell invasion assay kits)，包括 Oris™ 和 Oris™ Pro 細胞遷移(Oris™ Pro Cell Migration)和侵入檢測(Invasion Assays)。這些試劑盒為分析細胞對趨化因子(chemokines)、毒素(toxins)、藥物(drugs)和其他目標分析物的反應提供了一個即用型平台(ready-to-use platform)。
Oris™ Cell Migration Assay – Collagen I Coated 是一種可重複(reproducible)、靈敏(sensitive)且靈活(flexible assay)的檢測方法，可用於監測細胞遷移(cell migration)。該測定採用 96 孔板的格式，利用由醫用級矽膠(medicalgrade silicone)製成的 Oris™ 細胞播種塞(Oris™ Cell Seeding Stoppers)將細胞seeding限制在孔的外部環形區域。取下塞子會發現 2mm每個孔中心的直徑unseeded區域，即檢測區域，然後接種的細胞可以遷移到該區域。Oris™ 檢測掩膜應用於板底部，將可視化限制在檢測區域，僅允許細胞已遷移以待檢測（見圖 1）。 Oris™ Cell Migration Assay – Collagen I Coated 專為使用而設計使用任何市售的染色劑或標記技術。讀數可以通過顯微鏡或使用微孔板進行測量。Oris™ Cell Migration Assay – Collagen I Coated 系統設計用於貼壁細胞培養。這個檢測已成功用於 HT-1080、T47D、MCF10A、HeLa、HUVEC 和 MDA-MB-231 細胞株。
Oris™ Pro Cell Migration Assays系列產品
Oris™ Pro 細胞遷移檢測(Oris™ Pro Cell Migration Assays)適用於高通量篩選應用(high-throughput screening applications)的理想細胞遷移測定。Oris™ Pro Assays 使用水溶性生物相容性凝膠 (water-soluble biocompatible gel , BCG) 而不是 Oris™ Assays 中的物理矽屏障(physical silicon barrier)，為細胞遷移實驗(migration experiments)創建無細胞檢測區。細胞被播種在無毒 BCG 點周圍，在將培養基和細胞添加到每個孔後溶解，從而可以快速輕鬆地量化細胞遷移(cell migration)。Oris™ Pro Cell Migration Assays產品特點: 與任何貼壁細胞株具兼容性、提供組織培養處理板(tissue culture treated plates)或膠原蛋白 I 塗層(collagen I coated)表面與液體處理器(liquid handlers)、plate washers、高通量screening readers和其他高通量篩選工具兼容。
In each well, a water-soluble Bio-Compatible Gel (BCG) creates a central cell-free Detection Zone for cell migration experiments. Upon attachment of the cells the BCG dissolves within 60 minutes, allowing the cells to migrate into the Detection Zone at the center of each well.
在每個孔中，水溶性生物相容性凝膠 (water-soluble biocompatible gel , BCG) 為細胞遷移實驗創建了一個中央無細胞檢測區。細胞附著後，BCG 會在 60 分鐘內溶解，使細胞遷移到每個孔中心的檢測區。
96-well plates
384-well plates
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| Orisâ„¢ Pro 384 Cell Migration Assay – Tissue Culture Treated |  |
1-pack | View |
| Orisâ„¢ Pro 384 Cell Migration Assay – Tissue Culture Treated | |
5-pack | View |
| Orisâ„¢ Pro 384 Cell Migration Assay- Collagen I Coated | |
1-pack | View |
| Orisâ„¢ Pro 384 Cell Migration Assay- Collagen I Coated | |
5-pack | View |
Oris™ 細胞遷移和侵入檢測(Cell Migration and Invasion Assays)
遷移(Migration)是活細胞的基本特性，在正常發育(normal development)、免疫反應(immune response)以及炎症(immune response)和癌症轉移(disease processes)等疾病過程中起著至關重要的作用。測量細胞遷移的測定提供了對正常和疾病過程所涉及的機制的更詳細的了解，並且對於藥物和治療發現(therapeutic discovery)工作很重要。AMSBIO 提供 Oris™ 一系列細胞遷移檢測(Cell Migration Assays)，利用獨特的zone technology為您的實驗提供卓越的重現性(reproducibility)、準確性(accuracy)和精密度(precision)。通過識別細胞遷移抑制劑(cell migration inhibitors)和細胞遷移促進劑(cell migration promotors)，Oris™ 細胞遷移分析(Oris™ Cell Migration Assay)是推進藥物發現(drug discovery)、傷口癒合(wound healing)和癌症研究(cancer research)的有力工具。
Available with tissue culture treated plates, Collagen I coated plates, Fibronectin coated plates, Poly-L-Lysine (PLL) coated plates, Basement Membrane Extract (BME) coated plates or a Tricoated version (tissue culture treated, Collagen I, Fibronectin)
How do Oris™ Cell Migration Assays work?
Cells are seeded around a physical barrier: a silicon stopper. This barrier can remain in place after seeding for any desired length of time, facilitating extended adhesion times for the cells that need it. Following removal of the stoppers, the cells migrate into the detection zone and then be observed using a microscope, digital imaging system or fluorescence plate reader. Oris™ assays are the ideal alternative to scratch assays, reducing variability and providing quantitative cell migration data.
Single plate kits
Five plate kits
Oris™ 3D Cell Invasion Assay
In the Oris™ 3D Invasion Assay, silicon stoppers are used to create the central detection zone for cell invasion experiments; cells are suspended in a thick layer of collagen surrounding this zone. Following incubation, the stopper is removed and invading cells move into the detection zone, where they can be easily quantified using a microscope or a plate-reader.
Fig 1. Using the Oris™ 3D Embedded Invasion Assay, cells are suspended in a thick layer of collagen I surrounding a 2-mm circular central cell-free collagen I detection zone (left image). After several days incubation, invading cells move into the detection zone (right image) where they can be unambiguously quantified. Images are false-color composites of the detection zone stained for nuclei (red) and actin (green).
See all Cell Migration/Invasion Kits products
Amsbio台灣代理商-BRAND/AGENCY/代理品牌- Amsbio
AMSBIO於基因體學、蛋白質體學、細胞培養和幹細胞等領域均有涉略。 除了針對細胞增殖、貼附、轉移和侵入等研究細胞基礎功能之產品，另外還提供正常人及疾病患者的生物性樣本，例如乳癌、結腸癌等，以符合研究者的需求。產品線包含DNA/RNA/蛋白質/組織切片樣本、3D細胞培養、抗體及各式分析試劑盒等。
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| Orisâ„¢ Pro Cell Migration Assay – Collagen I Coated | |
1-pack | View |
| Orisâ„¢ Pro Cell Migration Assay – Collagen I Coated | |
5-pack | View |
| Orisâ„¢ Pro Cell Migration Assay – Tissue Culture Treated | |
1-pack | View |
| Orisâ„¢ Pro Cell Migration Assay – Tissue Culture Treated | |
5-pack | View |
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| Orisâ„¢ Pro 384 Cell Migration Assay – Tissue Culture Treated | |
1-pack | View |
| Orisâ„¢ Pro 384 Cell Migration Assay – Tissue Culture Treated | |
5-pack | View |
| Orisâ„¢ Pro 384 Cell Migration Assay- Collagen I Coated | |
1-pack | View |
| Orisâ„¢ Pro 384 Cell Migration Assay- Collagen I Coated | |
5-pack | View |