癌化特性之分析套組-Tube formation kit

trevigen提供 Cultrex® In Vitro Angiogenesis Assay Kit ，可以偵測血管細胞血管形成之inducers及inhibitors分析。微孔板上預包埋Trevigen專利的BME基質及其它組分蛋白，具有極低的細胞毒性，經嚴格的無細菌/真菌/支原體等驗證，接近真實體內環境；螢光定量具更高的精準度

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Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit

Catalog #: 3470-096-K 體外血管新生分析套組

癌化過程中具有血管新生之過程，藉以提供癌細胞養份及氧氣。血管新生作用是由內皮細胞所引導的。過程中細胞會分泌酵素來分解基底膜，造成細胞之遊走，經由一連串的增生分化後，最後形成管狀般的血管構造。trevigen提供 Cultrex® In Vitro Angiogenesis Assay Kit ，可以偵測血管細胞血管形成之inducers及inhibitors分析。 ​

Cultrex^® In Vitro Angiogenesis Assay Tube Formation Kit

Under permissive conditions, endothelial cells respond by self organizing into structures that resemble microvessels or tubes. Trevigen’s Cultrex® In Vitro Angiogenesis Assay Kit allows for the detection of inducers and inhibitors of endothelial cell tube formation. Cultrex^® Reduced Growth Factor (RGF) Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor, which gels at room temperature to form a reconstituted protein matrix comprised mainly of laminin, collagen IV, entactin, and heparin sulfate proteoglycan. Sulforaphane [1- isothiocyanato-(4R)- methylsulfinyl)-butane], found in broccoli and other cruciferous vegetables, is a naturally occurring cancer chemopreventive agent, and is provided as a control for inhibition of in vitro endothelial cell tube formation on Cultrex^® RGF BME. Calcein AM is provided for rapid and accurate measurement of cell viability and/or cytotoxicity, and kinetic analysis of tube formation. Trevigen’s Cultrex^® In Vitro Angiogenesis Assay Kit provides a cost-effective method for investigation of prospective angiogenesis inhibitors in a 96 well plate format.

COMPONENTS:

Basement Memrane Extract (BME) growth (3433-005-01) factor reduced

Calcein AM  (螢光顯微鏡)

Cell staining solution (倒立顯微鏡/相位差顯微鏡)

Sulphoraphane 10mM

100 µM Sulforaphane working solution

REFERENCES:

1. Folkman J. 2007. Angiogenesis: an organizing principle for drug discovery? Nat. Rev. Drug Discov. 6:273-286.
   2. Jaffe EA, Nachman RL, Becker CG, Minick CR. 1973. Culture of human endothelial cells derived from umbilical veins. J. Clin. Invest. 52:2745-2756.
   3. Folkman J, Haudenschild C. 1980. Angiogenesis in vitro. Nature 288:551- 556.
   4. Kleinman HK, McGarvey ML, Hassell JR, Star VL, Cannon FB, Laurie GW, Martin GR. 1986. Basement membrane complexes with biological activity. Biochemistry 25:312-318.
   5. Lawley TJ, Kubota Y. 1989. Induction to morphologic differentiation of endothelial cells in culture. J. Invest. Dermatol. 93:59S-61S.
   6. Ucuzian AA, Greisler HP. 2007. In vitro models of angiogenesis. World J. Surg. 31:654-663.
   7. Salcedo R, Zhang X, Young HA, Michael N, Wasserman K, Ma W-H, Martins-Green M, Murphy WJ, Oppenheim JJ. 2003. Angiogenic effects of prostaglandin E2 are mediated by up-regulation of CXCR4 on human microvascular endothelial cells. Blood 102:1966-1977.
   8. Mochizuki M, Philp D, Hozumi K, Suzuki N, Yamada Y, Kleinman HK, Nomizu M. 2007. Angiogenic activity of syndecan-binding laminin peptide AG73 (RKRLQVQLSIRT). Arch. Biochem. Biophys. 459:249-255.
   9. Arnaoutova, I. , J. George, H.K. Kleinman, and G. Benton. 2009. The endothelial cell tube formation assay on basement membrane turns 20: state of the science and the art. Angiogensis. 12(3):267-74.
   10. Arnaoutova I, and Kleinman HK. 2010. In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract. Nat Protoc. 5(4):628-35.

Kit Components:

更多原廠資料連結

http://www.trevigen.com/item/274/Cultrex_In_Vitro_Angiogenesis_Assay_Tube_Formation_Kit/

Trevigen的Cultrex體外血管生成分析試劑盒可進行內皮細胞管形成誘導劑和抑制劑的檢測.不含生長因數（RGF）的BME是從EHS腫瘤中純化出的可溶形式基底膜，其在室溫條件下凝固形成由層連蛋白、Ⅳ型膠原蛋白、Entactin蛋白以及肝素硫酸鹽糖蛋白形成的重構蛋白基質.在花椰菜和其他十字花科蔬菜中發現的萊菔硫烷，是一種天然的癌症化學抑制劑，被用於體外內皮細胞在Cultrex BME上血管形成的抑制劑陰性對照.鈣黃綠素-AM用於快速準確檢測細胞的生存能力和/或細胞毒性，以及血管形成的即時動力學分析.Trevigen Cultrex體外血管生成分析試劑盒以96孔方式為檢測潛在的血管生成抑制劑提供高效的方案.