PurKine™內毒素去除樹脂(套組) –PurKine™ Endotoxin Removal Kit (polymyxin B)

內毒素(Endotoxin)是位於革蘭氏陰性菌外膜的脂多醣(Endotoxinisthelipopolysaccharide, LPS）複合物。單個大腸桿菌(E.coli)含有200萬個LPS分子（2-20fg/細胞）。在實驗過程中，大量的內毒素(endotoxins)很容易污染耗材(consumables)、緩衝液(buffers)、樣品(samples)和其他下游產品。在體外，內毒素會導致細胞培養( cell-culture )中的各種問題在體內，內毒素可能會引起各種副作用，包括炎症反應(inflammatory response)、器官衰竭(organ failure)或敗血性休克在宿主生物體。 PurKine™內毒素去除試劑盒(PurKine™ Endotoxin Removal Kit )可以快速有效地消除內毒素(endotoxins )，從而消除內毒素。通過固定化多粘菌素B (immobilized Polymyxin B)，在含有蛋白質或藥理學重要成分的溶液中<0.1EU/mL，該物質以捕獲內毒素和防止毒性作用而聞名。

Endotoxin Removal resin; polymyxin B resin; polymyxin B

PurKine™內毒素去除樹脂(套組)  | PurKine™ Endotoxin Removal Kit (polymyxin B)貨號KTP2140  

PurKine™ Endotoxin Removal Kit (polymyxin B)套組包括預裝離心Column、buffer (1ml; 1mlx5)   (45-165 µm)

2,000,000 EU/mL to eliminate >99% of endotoxins.

• PurKine™ Endotoxin Removal polymyxin B Packed Column
• Regeneration buffer(10X)
• Equilibration buffer(10x)

PurKine™內毒素去除樹脂(PurKine™ Endotoxin Removal Resin) 可有效降低蛋白質樣品中的內毒素(Endotoxins)水平。內毒素(Endotoxins)，也稱為脂多醣(lipopolysaccharides)或LPS，是革蘭氏陰性細菌（Gram-negative bacteria）, 例如大腸桿菌(E. coli）的細胞膜成分(cell membrane components)。內毒素(Endotoxins)強烈影響DNA轉染至原代細胞(primary cells)和敏感培養細胞中，內毒素(Endotoxins)水平升高導致轉染效率(transfection efficiencies)急劇降低。樹脂(Resin)由90μm交聯的4％瓊脂糖珠(cross-linked 4% agarose)組成，且由修飾的多粘菌素B(modified polymyxin B)與之偶聯。該產品組合具優化(optimization)，以實現最大的蛋白質產量(protein yield)，穩定性(stability)和溶解性(solubility)。測試確認，至少重複使用五次後。性能不會降低。PurKine™內毒素去除系列產品，有提供樹脂(PurKine™內毒素去除樹脂, 貨號BMR2140)、預裝離心柱(PurKine™內毒素去除預裝離心柱, BMC2140) 和試劑盒(PurKine™內毒素去除樹脂(套組)  (polymyxin B), KTP2140) 三種商品形式提供。

產品描述：

PurKine™內毒素去除試劑盒(PurKine™ Endotoxin Removal Kit (polymyxin B), KTP2140) 旨在將蛋白質樣品中的內毒素(Endotoxins)水平降低≥99％，同時最大限度地提高蛋白質回收率。樹脂(Resin)由90μm交聯的4％瓊脂糖珠組成，修飾的多粘菌素B（PMB）已與之偶聯。測試證實其性能等於或超過其他供應商的流行多粘菌素B（PMB）樹脂，並且至少重複使用五次後，性能不會降低。操作手冊PurKine™ Endotoxin Removal Kit (polymyxin B)

PurKine™ Endotoxin Removal Resin has been designed to reduce endotoxin levels in protein samples by ≥99% while maximizing protein recovery. The Resin consists of 90μm beads of cross-linked 4% agarose, to which modified polymyxin B (PMB) has been coupled. Tests confirm that performance equals or exceeds popular polymyxin B (PMB) resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.

Characteristics of ToxinEraser™ Endotoxin Removal Resin

Size	1 ml in pre-packed column
Binding Capacity	Up to 2,000,000 EU/ml resin
Ligand	modified polymyxin B (Polymixin B, PMB)
pH Stability	pH7-8
Support Matrix	4% cross-linked agarose, spherical beads
Mean Particle Size	90 μm
Storage	2°C to 8°C (Do not freeze.)
Formulation	Liquid solution with 50% slurry in 20% ethanol.
Kit components	• PurKine™ Endotoxin Removal polymyxin B Packed Column • Regeneration buffer(10X) • Equilibration buffer(10x)
Features & Benefits	• High capacity—More than 2,000,000 EU/mL to eliminate >99% of endotoxins. • High performance—Protein recovery is more than 85%.Reducing final EU concentration to 0.1 EU/mL • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits.