【PurKine™內毒素】樹脂、預製Column、內毒素去除套組-Abbkine品牌代理商
PurKine™內毒素去除樹脂(套組) –PurKine™ Endotoxin Removal Kit (polymyxin B)
PurKine™ Endotoxin Removal Kit (polymyxin B)套組包括預裝離心Column、buffer (貨號KTP21400)
• PurKine™ Endotoxin Removal polymyxin B Packed Column
• Regeneration buffer(10X)
• Equilibration buffer(10x)
A. 內毒素去除原理
PurKine™內毒素去除樹脂(PurKine™ Endotoxin Removal Resin) 可有效降低蛋白質樣品中的內毒素(Endotoxins)水平。內毒素(Endotoxins),也稱為脂多醣(lipopolysaccharides)或LPS,是革蘭氏陰性細菌(Gram-negative bacteria), 例如大腸桿菌(E. coli)的細胞膜成分(cell membrane components)。內毒素(Endotoxins)強烈影響DNA轉染至原代細胞(primary cells)和敏感培養細胞中,內毒素(Endotoxins)水平升高導致轉染效率(transfection efficiencies)急劇降低。樹脂(Resin)由90μm交聯的4%瓊脂糖珠(cross-linked 4% agarose)組成,且由修飾的多粘菌素B(modified polymyxin B)與之偶聯。該產品組合具優化(optimization),以實現最大的蛋白質產量(protein yield),穩定性(stability)和溶解性(solubility)。測試確認,至少重複使用五次後。性能不會降低。
B. 內毒素去除產品種類
PurKine™內毒素去除系列產品,有提供樹脂(PurKine™內毒素去除樹脂, 貨號BMR21400)、預裝離心柱(PurKine™內毒素去除預裝離心柱, BMC21400) 和試劑盒(PurKine™內毒素去除樹脂(套組) (polymyxin B), KTP21400) 三種商品形式提供。
C. 產品描述: PurKine™內毒素去除試劑盒(PurKine™ Endotoxin Removal Kit (polymyxin B), KTP21400) 旨在將蛋白質樣品中的內毒素(Endotoxins)水平降低≥99%,同時最大限度地提高蛋白質回收率。樹脂(Resin)由90μm交聯的4%瓊脂糖珠組成,修飾的多粘菌素B(PMB)已與之偶聯。測試證實其性能等於或超過其他供應商的流行多粘菌素B(PMB)樹脂,並且至少重複使用五次後,性能不會降低。操作手冊PurKine™ Endotoxin Removal Kit (polymyxin B)
PurKine™ Endotoxin Removal Resin has been designed to reduce endotoxin levels in protein samples by ≥99% while maximizing protein recovery. The Resin consists of 90μm beads of cross-linked 4% agarose, to which modified polymyxin B (PMB) has been coupled. Tests confirm that performance equals or exceeds popular polymyxin B (PMB) resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.
- FormulationLiquid solution with 50% slurry in 20% ethanol.
- Kit components• PurKine™ Endotoxin Removal polymyxin B Packed Column
• Regeneration buffer(10X)
• Equilibration buffer(10x) - Features & Benefits• High capacity—More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
• High performance—Protein recovery is more than 85%.Reducing final EU concentration to 0.1 EU/mL
• Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits. - Storage instructionsStable for one year at 2-8°C from date of shipment. Do not freeze.