【立百病毒 (Nipah Virus, NiV) 抗體、蛋白質和ELISA試劑盒 】Nipah Virus Antibodies, Proteins & ELISA Kits
立百病毒抗體、蛋白質和ELISA試劑盒 –Nipah Virus ELISA Kits
Infectious Disease ELISA Kits
什麼是立百病毒？(What is Nipah Virus? )
立百病毒（Nipah Virus , NiV）是一種人畜共通病毒( zoonotic virus )，屬於副黏病毒科亨尼帕病毒屬 (the Paramyxoviridae family and the genus Heniparvirus)。 NiV於1988年首次在馬來西亞(Malaysia )被發現，其主要宿主是狐蝠屬蝙蝠( Pteropus bat)。受感染的豬、蝙蝠(bats)和水果是NiV傳播給人類的途徑。
立百病毒（Nipah Virus , NiV）的結構有助於其致病性和複製能力。尼帕病毒（Nipah Virus , NiV）包含核衣殼蛋白（nucleocapsid proteins, N）、基質蛋白（matrix proteins , M）、磷蛋白（phosphoproteins , P）、大分子蛋白（large proteins , L）、融合蛋白（ fusion proteins, F）和醣蛋白（glycoproteins, G）。此外，尼帕病毒（Nipah Virus , NiV）還包含非結構蛋白(non-structural proteins)，例如C、V和W蛋白。
立百病毒（Nipah Virus , NiV）已被世界衛生組織（世衛組織）列入優先病原體名單，一旦爆發疫情，可能需要迅速研究和開發。針對這些病毒成分進行分析的能力有助於治療方法的研發和疫苗的生產。立百病毒重組蛋白: 研究表明，立百病毒（NiV）可透過阻斷Janus激酶-訊號傳導及轉錄活化因子（JAK – STAT）路徑來抑制其目標宿主中的干擾素訊號傳導。此外，研究主要集中在NiV的F蛋白和G糖蛋白上，它們是病毒細胞黏附和入侵宿主所必需的。 G糖蛋白與上皮細胞上的細胞受體Ephrin-B2結合，這種相互作用會引起構象變化，進而促進膜融合。 NiV蛋白V可增加UBX結構域蛋白1（UBXN1）的表達，而UBXN1負調控RIG-I樣訊號傳導。在NiV病毒入侵後以及感染進展過程中，均可觀察到IgG和IgM抗體反應。
立百病毒ELISA試劑盒 Nipah Virus Glycoprotein (G) Human IgG ELISA Kit
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 4-8 weeks |
| Species | Human, Nipah Virus |
| Compatible Sample Types | Plasma, Serum |
| Gene Symbols | IgG |
| Protein Name / Synonyms | IgG; Nipah Virus Glycoprotein (G) |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Indirect |
| Method Of Detection | Colorimetric |
| Research Area | Infectious Disease, Hemorrhagic Fever |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -20°C |
Introduction
This in-vitro ELISA kit employs an indirect ELISA method. Standard 96-well plates are coated with the pathogen antigen, which combines with the corresponding antibody present in a sample and positive control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA/G/M antibody is added, a complex forms between the biotinylated secondary antibody and human antibody bound to the protein-coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-pathogen antigen antibody present. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Control consists of an anti-pathogen antigen antibody. A biotinylated anti-positive control antibody is added to the positive control wells and treated identically to the samples. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.