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【Lactate乳酸鹽測定】Lactate Assay Kit Biovision K607/K627/K668 Abcam (Biovision)

【乳酸鹽測定試劑盒】Lactate Assay Kit-Abcam (Biovision)

metabolism

原理介紹乳酸(CH 3 CH(OH)COO-)在許多生物過程中起重要作用。異常高濃度的乳酸(Lactate )與諸如糖尿病( diabetes )和乳酸酸中毒(lactate acidosis)等疾病狀態有關。L(+) – 乳酸鹽(L(+)-Lactate)是在人類中間代謝中形成的乳酸鹽之主要立體異構體 (stereo-isomer ),並且存在於血液中。 D( – ) – 乳酸鹽也存在但僅在L(+) – 乳酸鹽濃度的約1-5%。 Biovision提供一系列糖解途徑分析套組;其中乳酸鹽測定試劑盒,提供兩種吸收光之產品作選擇;乳酸鹽測定試劑盒(Lactate Assay Kit I, 貨號K607-100) 中,乳酸特異性地與酶混合物反應以產生產物,其與乳酸鹽探針相互作用以產生顏色,可以ELISA呈色於(570nm)波段分析;或螢光(在Ex / Em = 535 / 587nm處)測量。 該試劑盒為檢測生物樣品中的L(+) – 乳酸鹽提供了便利的方法,可測量樣品:血液循環(blood circulation),細胞( cells),培養基(culture mediums),發酵培養基( fermentation mediums)等。不需要預處理或純化樣品。 該試劑盒可檢測0.001-10mM的各種乳酸鹽樣品 (Lactate samples)。測量培養基(culture mediums)時,使用不含乳酸鹽( Lactate ) 和/或丙酮酸鹽( pyruvate )的培養基。含有FBS的培養基應脫蛋白( deproteinized ) 移去LDH避免降解乳酸鹽。在乳酸鹽測定試劑盒(Lactate Assay Kit II, K627-100) 中,提供了替代波長 (450 nm波長濾光片),乳酸鹽被乳酸脫氫酶氧化,產生與探針相互作用產生顏色(λmax= 450nm)的產物。 該試劑盒檢測生物樣品中的L(+) – 乳酸鹽,例如血清或血漿,細胞,培養物和發酵培養基。 不需要預處理或純化樣品。 它在各種樣品中檢測到0.02 mM – 10 mM乳酸。PicoProbe™D-Lactate D-乳酸檢測 | 貨號K668-100 D-乳酸(D-Lactate)是胃腸系統( gastrointestinal system)中微生物的無氧糖酵解( anaerobic glycolysis )的結果,以及乙二醛酶系統( glyoxalase system)對甲基乙二醛(methylglyoxal )的解毒(detoxification)產物。 D-乳酸異常水平的存在與一系列病理狀況有關,例如糖尿病( diabetes)和闌尾炎(appendicitis)。 BioVision的PicoProbe™D-Lactate Assay試劑盒具有簡便性和靈敏度,可適應高通量研究。 該測定法酶促氧化D-乳酸鹽(D-Lactate ),產生螢光信號( fluorescent signal , Ex / Em = 535 / 587nm)。 信號與D-乳酸鹽的量成正比。 該檢測試劑盒可檢測含有低至0.1μMD-乳酸鹽的樣品。

biovision商品插圖

產品應用: 

—檢測不同生物樣品之乳酸鹽含量

—分析不同細胞之乳酸鹽代謝

—糖尿病研究

產品特點:

—K607可選擇呈色Absorbance (570 nm)或螢光Fluorescence (Ex/Em 535/587 nm)兩種型態偵測; K627可選擇450 nm波長濾光片

—K607檢測0.001-10mM範圍之乳酸鹽含量; K627檢測0.02 mM – 10 mM乳酸。

—可測量樣品:血液循環(blood circulation),細胞( cells),培養基(culture mediums),發酵培養基( fermentation mediums)等。

相關試劑 Lactate乳酸呈色螢光分析套組|  貨號K607-100

Lactate Colorimetric/Fluorometric Assay Kit

原理介紹Lactate乳酸呈色螢光分析套組,一組產品可選擇呈色Absorbance (570 nm)或螢光Fluorescence (Ex/Em 535/587 nm)兩種型態偵測;可用於細胞培養液、尿液、血漿、或液體型式的來源。偵測的靈敏度為方法0.001-10 Mm; 快速簡單,操作時間大約40分鐘。異常高濃度的乳酸(Lactate )與諸如糖尿病( diabetes )和乳酸酸中毒(lactate acidosis)等疾病狀態有關。L(+) – 乳酸鹽(L(+)-Lactate)是在人類中間代謝中形成的乳酸鹽之主要立體異構體 (stereo-isomer ),並且存在於血液中。 D( – ) – 乳酸鹽也存在但僅在L(+) – 乳酸鹽濃度的約1-5%。 在乳酸鹽測定試劑盒(Lactate Assay Kit, K607) 中,乳酸特異性地與酶混合物反應以產生產物,其與乳酸鹽探針相互作用以產生顏色,可以ELISA呈色於(570nm)波段分析;或螢光(在Ex / Em = 535 / 587nm處)測量。 該試劑盒為檢測生物樣品中的L(+) – 乳酸鹽提供了便利的方法,可測量樣品:血液循環(blood circulation),細胞( cells),培養基(culture mediums),發酵培養基( fermentation mediums)等。不需要預處理或純化樣品。 該試劑盒可檢測0.001-10mM的各種乳酸鹽樣品 (Lactate samples)。測量培養基(culture mediums)時,使用不含乳酸鹽( Lactate ) 和/或丙酮酸鹽( pyruvate )的培養基。 含有FBS的培養基應脫蛋白( deproteinized ) 移去LDH避免降解乳酸鹽。

 

Lactate乳酸呈色螢光分析套組 | K607-100內容物

Lactate Assay Buffer

Lactate Probe (in DMSO)

Lactate Enzyme Mix

L(+)-Lactate Standard (100 nmol/µl)

引用文獻

1. Hyun Jik Lee, O-cyclic phytosphingosine-1-phosphate stimulates HIF1α-dependent glycolytic reprogramming to enhance the therapeutic potential of mesenchymal stem cells. Cell Death Dis, Aug 2019;  31383843.
2. Ran Jing, A Screen Using iPSC-Derived Hepatocytes Reveals NAD+ as a Potential Treatment for mtDNA Depletion Syndrome. Cell Rep, Nov 2018;  30404003.
3. Guang-Zhi Jin, et al., ( 2018) Phosphoglucomutase 1 inhibits hepatocellular carcinoma progression by regulating glucose trafficking, PLoS Biology, Oct. 2018, 30335765
4. Qi Wu, et al., ( 2018) Tumour-originated exosomal miR-155 triggers cancer-associated cachexia to promote tumour progression, Molecular Cancer, Oct. 2018, 30359265
5. Ashley R. Maiuri, et al., (2018) Inflammation-induced DNA methylation of DNA polymerase gamma alters the metabolic profile of colon tumors, Cancer Metab, Jul. 2018, 30002826

相關試劑 乳酸比色測定試劑盒II Lactate Colorimetric Assay Kit II | 貨號K627-100

產品描述:乳酸(CH 3 CH(OH)COO-)在許多生物過程中起重要作用。 異常高濃度的乳酸與疾病狀態如糖尿病和乳酸酸中毒等有關.L(+) – 乳酸鹽是人體中間代謝中形成的主要乳酸鹽立體異構體,存在於血液中。 D( – ) – 乳酸鹽也存在,但僅在L(+) – 乳酸鹽濃度的約1-5%。 在乳酸鹽測定試劑盒中,乳酸鹽被乳酸脫氫酶氧化,產生與探針相互作用產生顏色(λmax= 450nm)的產物。 該試劑盒檢測生物樣品中的L(+) – 乳酸鹽,例如血清或血漿,細胞,培養物和發酵培養基。 不需要預處理或純化樣品。 它在各種樣品中檢測到0.02 mM – 10 mM乳酸。

商品需用儀器測量  酵素免疫分析儀請按此 (標配405、450、492、630 nm, 可擴增至7個濾片)

RT-6900

 

 

 

 

 

 

 

Cat # +Size K627-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian
Applications Kit detects 0.02 mM – 10 mM lactate in various samples.
Features & Benefits • Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of Lactate
Kit Components • Lactate Assay Buffer
• Lactate Enzyme Mix
• L(+)-Lactate Standard (100 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

引用文獻

Zhimin Ou, A GPR17-cAMP-Lactate Signaling Axis in Oligodendrocytes Regulates Whole-Body Metabolism. Cell Rep, March 2019;  30865888.
Mariana Guerra-Maupome, Aerosol vaccination with Bacille Calmette-Guerin induces a trained innate immune phenotype in calves. PLoS One, Feb 2019; 30794653.
Myung Jin Son, A novel and safe small molecule enhances hair follicle regeneration by facilitating metabolic reprogramming. Exp Mol Med, Dec 2018; 30523246.
Qi Cao, Quantification of Hepatic Lipid Using 7.0T Proton Magnetic Resonance Spectroscopy and Computed Tomography in Mild Alcoholic Steatotic Mice. J Liver, Dec 2018;  30906674.
Manuela Verri, Plasma energy substrates at two stages of Alzheimer’s disease in humans. Int J Immunopathol Pharmacol, Dec 2018; PMC6309029.
Ling, Z. et al., (2017) miR-361-5p modulates metabolism and autophagy via the Sp1-mediated regulation of PKM2 in prostate cancer, Oncology Reports, Jul.2017, 38(3), 1621-1628
Sun, X. et al., (2017) MicroRNA-143 suppresses oral squamous cell carcinoma cell growth, invasion and glucose metabolism through targeting hexokinase 2, Bioscience reports, Jun.2017, 37(3), BSR20160404
Elmorsy, E. et al. (2017) Adverse effects of anti-tuberculosis drugs on HepG2 cell bioenergetics Hum Exp Toxicol. 2017 Jun;36(6):616-625.
Sun, Lucia et al. (2017) MicroRNA-143 suppresses oral squamous cell carcinoma (OSCC) cell growth, invasion and glucose metabolism through targeting Hexokinase2, Biosci Rep. 2017 Jun 8;37(3).
Miyata, Saori et al. (2017) Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation, Int J Nanomedicine. 2017 Apr 4;12:2703-2716.

相關試劑 PicoProbe™D-Lactate D-乳酸檢測 | 貨號K668-100

PicoProbe™ D-Lactate Fluorometric Assay Kit

產品描述:D-乳酸(D-Lactate)是胃腸系統( gastrointestinal system)中微生物的無氧糖酵解( anaerobic glycolysis )的結果,以及乙二醛酶系統( glyoxalase system)對甲基乙二醛(methylglyoxal )的解毒(detoxification)產物。 D-乳酸異常水平的存在與一系列病理狀況有關,例如糖尿病( diabetes)和闌尾炎(appendicitis)。 BioVision的PicoProbe™D-Lactate Assay試劑盒具有簡便性和靈敏度,可適應高通量研究。 該測定法酶促氧化D-乳酸鹽(D-Lactate ),產生螢光信號( fluorescent signal , Ex / Em = 535 / 587nm)。 信號與D-乳酸鹽的量成正比。 該檢測試劑盒可檢測含有低至0.1μMD-乳酸鹽的樣品。

Cat # +Size K668-100
Size 100 assays
Detection Method Fluorescence (Ex/Em= 535/587 nm)
Applications Measurement of D-Lactate in various tissues/cells/biological fluids
Analysis of D-Lactate in pathological conditions
Mechanistic study of the glyoxalase system
Features & Benefits • Simple, highly sensitive, and high-throughput adaptable.
• The assay kit can detect samples containing as low as 0.1 µM D-Lactate.
Kit Components • D-Lactate Assy Buffer
• PicoProbe™ (in DMSO)
• D-Lactate Enzyme Mix (Lyophilized)
• D-Lactate Substrate Mix (Lyophilized)
• D-Lactate Standard (100 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
 Catalog #  Product Name+  Size
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