【D-螢光素(D-luciferin)水溶性鈉鹽】CAS#103404-75-7 MedChemExpress(MCE)貨號HY-12591
MCE (MedChem Express) -D-螢光素(D-luciferin)
MCE (MedChemexpress) 總部位於美國新澤西，提供種類齊全的化學及生化研究用產品，包括創新的生命科學試劑，reference compounds (標準化合物)，APIs (原料藥) 及Natural compounds (天然化合物)以提供實驗室或科學研究之用，MedChemExpress(MCE)產品50000+ 特異性抑制劑、化合物庫、重組蛋白，專注於信號通路和疾病研究領域。全球文獻廣泛引用，質控體系嚴格，品質100%。官網連結 https://www.medchemexpress.com
D-Luciferin sodium purchased from MCE. Usage Cited in: Acta Pharm Sin B. 12 March 2022.
Tumor growth is monitored by bioluminescence imaging. Prior to imaging, D-luciferin substrate is injected intraperitoneally into the mice (150 mg/kg per mouse).
D-螢光素(D-luciferin)是螢光素酶 (Luc) 的天然底物，可催化螢火蟲典型的黃綠光的產生。來自該反應的 560 nm 化學發光在幾秒鐘內達到峰值，當底物螢光素(luciferin)過量存在時，光輸出與螢光素酶濃度成正比。螢光素酶 (luc) 基因是用於研究和藥物篩選的常用報告基因。化學發光技術幾乎沒有背景，使 luc 報告基因成為檢測低水平基因表達的理想選擇。在標準閃爍計數器中可以可靠地測量低至 0.02 pg 的螢光素酶。除了作為基因表達報告基因的作用外，螢光素酶還常用於極其敏感的 ATP 檢測。我們提供螢火蟲螢光素酶 (HY-P1004)、螢光素游離酸 (HY-12591A) 及其水溶性鈉鹽 (HY-12591) 和鉀鹽 (HY-12591B)。。
D-螢光素(D-luciferin) | 水溶性鈉鹽CAS#103404-75-7 MedChemExpress(MCE)貨號HY-12591
D-(-)-Luciferin sodium; Firefly luciferin sodium; Beetle Luciferin sodium
D-Luciferin sodium purchased from MCE. Usage Cited in: Cell Mol Gastroenterol Hepatol. 2021;12(3):839-856.
HEK293T cells are seeded into a 6-well plate and transfected with in vitro synthesized mRNA containing the luciferase gene by Lipofectamine 2000. Luciferase substrate D-Luciferin sodium salt (1 mM) is added into the culture medium immediately after transfection. Luciferase activity is detected on the GloMax 96 Microplate Luminometer.
D-Luciferin sodium purchased from MCE. Usage Cited in: Pharmacol Res. 2021 Mar 2;105527. [Abstract]
After cell inoculation for 7 days, mice are injected intraperitoneally with D-luciferin substrate at 150 mg/kg and anesthetized 10% chloral hydrate (5 mL/kg).
D-Luciferin sodium purchased from MCE. Usage Cited in: Cell Death Dis. 2020 Sep 17;11(9):765. [Abstract]
At Day 10, 25, and 31, mice are anesthetized with isoflurane and then injected with 75 mg/kg D-Luciferin solution for imaging.
