【BCA蛋白定量分析套組】 VisualProtein貨號BC03-500
Dual-Range BCA Protein Assay Kit
BCA最常用的測量蛋白質濃度(protein concentration)的方法。
Dual-Range™ BCA 蛋白質檢測試劑盒(Dual-Range™ BCA Protein Assay Kit, BC03-500)是最常用的蛋白質檢測方法。與 Lowry 方法一樣，該測定依賴於蛋白質對 Cu2+ 離子的還原。通過與二辛可寧酸(bicinchoninate)反應轉化為紫色物質來檢測由此形成的 Cu+。 Dual-Range™ BCA Protein Assay Kit 與許多去污劑兼容，但與還原劑(reducing agents)如 DTT、DTE 和 2-巰基乙醇(2-Mercaptoethanol)等不兼容。二辛可寧酸 (bicinchoninic acid , BCA)，試劑 A 含有 BCA，試劑 B 則含有硫酸銅 (CuSO4)。 使用前混和兩種試劑，在鹼性環境下，蛋白質可將二價銅離 子 Cu2+ 還原為兩個單價銅離子 Cu+，而一個 Cu+ 可螯合兩 個 BCA 分子，使試劑顏色由蘋果綠轉變為紫色，此專一性呈 色與蛋白質濃度成正比，透過檢測 562nm 的吸光值，並比對 標準蛋白 ( 例如：BSA) 的校正曲線，可取得蛋白質濃度定量 數據。 用於 5–2,000 μg/mL 濃度範圍內的總蛋白質的比色檢測和定量
● 線性範圍廣，可達到 20-2,000 μg/mL
● BCA 對介面活性劑 (detergent) 具有高耐受性，不易受其影響
● 測定快速，可於 30 分鐘完成測定，比 Lowry 法快 4 倍
● 室溫保存，效期 24 個月
● 台灣生產，品質保證
訂購資訊
BC03-500 Dual-Range BCA Protein Assay Kit
> Reagent A 500 mL
> Reagent B 12 mL
BSA Standard (2 mg/mL) 1 mL x 10
Reference:
1. Young, Guang-Huar, et al. “The functional role of hemojuvelin in acute ischemic stroke.” Journal of Cerebral Blood Flow & Metabolism (2019): 0271678X19861448.
2. Chen, Sheng-Yi, et al. “Antioxidant and anti-inflammatory activities and bioactive compounds of the leaves of Trichodesma khasianum clarke.” Industrial Crops and Products 151 (2020): 112447.
3. Wang, Guan-Yu, et al. “Protective effect of rosmarinic acid-rich Trichodesma khasianum Clarke leaves against ethanol-induced gastric mucosal injury in vitro and in vivo.” Phytomedicine (2020): 153382.
4. Young, Guang‐Huar, et al. “Modulation of adenine phosphoribosyltransferase‐mediated salvage pathway to accelerate diabetic wound healing.” The FASEB Journal 35.3 (2021): e21296.
5. Leyton, Esteban, et al. “DEF8 and Autophagy-Associated Genes are Altered in Mild Cognitive Impairment, Probable Alzheimer’s Disease Patients and a Transgenic Model of the Disease.” Journal of Alzheimer’s Disease Preprint (2021): 1-16.
6. Hsu, Jen‐Ying, et al. “Aqueous extract from Pepino (Solanum muricatum Ait.) leaves ameliorated insulin resistance, hyperlipidemia, and hyperglycemia in mice with metabolic syndrome.” Journal of Food Biochemistry 44.12 (2020): e13518.
| BR01-500 | Bradford 蛋白質定量試劑 | Dual-Range Bradford Reagent (1X), 500 ml , | |
| BR05-500 | 5X Bradford 蛋白質定量試劑 | Dual-Range Bradford Reagent (5X), 500ml | |
| BR05-500-K | 5X Bradford 蛋白質定量試劑組 |
Dual-Range Bradford Protein Assay Kit, (500 ml +10* 2mg/ml BSA standard) |
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| BP01-1L | 西方墨點法遮蓋液 | BlockPRO Blocking Buffer, 500mlx2 | |
| BF01-1L | 西方墨點法遮蓋液-Protein Free | BlockPRO Protein-Free Blocking Buffer, 500mlx2 | |
| BF20-50P | 西方墨點法遮蓋液-Protein Free |
BlockPRO Protein-Free Blocking Buffer, 20X, 20包，每包2.5 mL， 使用前加入500mL ddH2O 回溶成 1X |
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| 西方墨點法遮蓋液-Protein Free, 20X | |||
| BM01-500 | 西方墨點法遮蓋液-1分鐘配方 | BlockPRO 1 Min Protein-Free Blocking Buffer, 500ml | |
| SP01-500 | 抗體去除試劑 | StripPRO 1 Min Stripping Buffer, 500ml | |
| LF01-500 | 冷光試劑組- Prime | LumiFlash Prime Chemiluminescent Substrate,( 250mL solnA + 250mL soln B) | |
| LF08-500 | 冷光試劑組- Ultima | LumiFlash Ultima Chemiluminescent Substrate, (250mL solnA + 250mL soln B) | |
| LF16-500 | 冷光試劑組- Infinity | LumiFlash Infinity Chemiluminescent Substrate, (250mL solnA + 250mL soln B) | |
| VC01-250 | 預染蛋白質標準液 | VisColorTM Pre-Stained Protein Marker, 250ul x 1 | |
| VC01-500 | 預染蛋白質標準液 | VisColorTM Pre-Stained Protein Marker, 250ul x 2 | |
| VC03-250 | 全範圍預染蛋白質標準液 |
VisColorTM Full-Range Pre-Stained Protein Marker, 250ul x 1
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| VC03-500 | 全範圍預染蛋白質標準液 |
VisColorTM Full-Range Pre-Stained Protein Marker, 250ul x 2
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| LH03-10 | 冷光筆 |
LuminolPen HRP system, (約 150 張 Western blot’s membranes 使用) |
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| LH03-50 | 冷光筆 |
LuminolPen HRP system, (約1000 張 Western blot’s membranes 使用) |
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| LH05-10 | 冷光筆 EZ版 | LuminolPen EZ HRP System, (約 150 張 Western blot’s membranes 使用) | |
| LH05-50 | 冷光筆 EZ版 | LuminolPen EZ HRP System, (約 1000 張 Western blot’s membranes 使用) | |
| RB10-1L | SDS-PAGE 電泳緩衝液 | SDS-PAGE Running Buffer , 10X (Tris-Glycine, pH 8.3), 500 ml *2 | |
| RB500P | SDS-PAGE 電泳緩衝液濃縮包 |
SDS-PAGE Running Buffer , 10X (Tris-Glycine, pH 8.3), (20包，每 包須於使用前加入500mL ddH2O 回溶成 1X) |
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| WTB10-1L | Western Blot Transfer Buffer | Western Blot Transfer Buffer, 10X (Tris-Glycine, pH 8.3), 500 ml *2 西方墨點法轉印緩衝液 | |
| WTB500P | 西方墨點法轉印緩衝液濃縮包 |
Western Blot Transfer Buffer,(20包，每包須於使用前加入500mL ddH2O 回溶成 1X) |
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| TBS10-1L | TBS緩衝液 | TBS Buffer, 10X, 500 mL*2 | |
| TBS200P | TBS 緩衝液濃縮包 |
TBS Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) |
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| TBST10-1L | TBST 緩衝液 | TBS Tween-20 Buffer, 10X, 500 mL*2 | |
| TBST200P | TBST 緩衝液濃縮包 |
TBS Tween-20 Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) |
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| PBS10-1L | PBS 緩衝液 | PBS Buffer, 10X , 500 mL*2 | |
| PBS200P | PBS 緩衝液 | PBS Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) | |
| PBST10-1L | PBST 緩衝液 | PBS Tween-20 Buffer, 10X, 500 mL*2 | |
| PBST200P | PBST 緩衝液 |
PBS Tween-20 Buffer, 25X, (30包，每包須於使用前加入200 mL ddH2O 回溶成 1X) |
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| SBN06-15 | 6x Laemmli SDS Sample Buffer, non-reducing | 6x Laemmli SDS Sample Buffer, non-reducing, 15ml | |
| SBR06-15 | 6x Laemmli SDS Sample Buffer, reducing | 6x Laemmli SDS Sample Buffer, reducing, 15ml | |
| VP01-125 | 全蛋白質負染色試劑組 |
VisPRO 5 Minutes Protein Stain Kit, (125mL soln 1 + 125mL soln2) |
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| VP01-500 | 全蛋白質負染色試劑組 |
VisPRO 5 Minutes Protein Stain Kit, (500mL soln 1 + 500mL soln 2) |
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| VP05-125 | 全蛋白質負染色試劑組- 五倍濃縮液 |
5x VisPRO 5 Minutes Protein Stain Kit, (125mL soln 1 + 125mL soln 2)
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| VP05-500 | 全蛋白質負染色試劑組- 五倍濃縮液 | 5x VisPRO 5 Minutes Protein Stain Kit, (250mL soln 1 + 250mL soln 2) | |
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PP03-2C
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磷酸化蛋質萃取試劑組 | PhosPRO Phosphoprotein Enrichment Kit, (2 pre-packed column + 2mL Lysis buffer+250mL System buffer) +(100mL Elution buffer+100 mL Stringent buffer stock) | |
| PP03-6C | 磷酸化蛋質萃取試劑組 |
PhosPRO Phosphoprotein Enrichment Kit, (6 pre-packed column + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock ) |
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PP03-5E
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磷酸化蛋質萃取試劑組 |
PhosPRO Phosphoprotein Enrichment Kit, (3 mL Resin + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock) |
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PP05-2C
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PhosPRO Phosphoprotein Purification Kit, (2 pre-packed column + 2mL Lysis buffer+250mL System buffer) +(100mL Elution buffer+100 mL Stringent buffer stock) | ||
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PP05-6C
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磷酸化蛋白質純化試劑組 | PhosPRO Phosphoprotein Purification Kit, (6 pre-packed column + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock) 磷酸化蛋白質純化試劑組 | |
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PP05-5E
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磷酸化蛋白質純化試劑組 | PhosPRO Phosphoprotein Purification Kit, (3 mL Resin + 2mL Lysis buffer+250mL System buffer) + (100mL Elution buffer+100 mL Stringent buffer stock ) | |
| EP05-30 | 全蛋白質萃取試劑 | ExtractPRO Protein Extraction Reagent | |
| FP01-5 | 胞器蛋白質分離試劑組 | FracPRO Protein Fractionation Kit, (10mL Soln1+ 10mL Soln2 +7.5mL Soln3+1.5mL Soln4) | |
| FP01-50 | 胞器蛋白質分離試劑組 | FracPRO Protein Fractionation Kit, (100mL Soln1 + 100mL Soln2+ 75mL Soln3 +15mL Soln4) | |
| HB01-1L | 促融合瘤細胞生長因子 | HybriMore, Hybridoma cloning factor, for 1L medium dilution | |
| IF01-4N | 快速免疫佐劑 | ImmunoFast Adjuvant, for 4 injections to mouse | |
| DG03-1P | DIGE蛋白質標記試劑組 | DIGE-Kit, (Cy2 400pmole + Cy3 400pmole + Cy5 400pmole+ 20ml DMF) | |
| GLP01 | 負染膠片光箱 | Gel Lighting Plate 21 x 29.7 cm(A4) | |
| BLP01 | 正染膠片光箱 | Band Lighting Plate 21 x 29.7 cm(A4) | |
| BSB01 | 黑染盒 | Black Staining Box 13 x 13 x 4 cm (For mini gel) | |
| BSB02 | 黑染盒 | Black Staining Box 17.5 x 17.5 x 4.5 cm (For regular gel) | |
| BSB03 | 黑染盒 | Black Staining Box 20 x 24 x 5 cm | |
| BSB04 | 黑染盒 | Black Staining Box 27 x 27 x 5 cm |