血尿素氮（Blood Urea Nitrogen , BUN）檢測試劑盒 | 貨號abx090684

血尿素氮（Blood Urea Nitrogen , BUN）檢測試劑盒可用於檢測血清(serum)，血漿(plasma)，細胞培養基(cell culture media)，細胞(cells)和組織勻漿樣品(tissue homogenate samples)中的尿素氮(urea nitrogen)。消化蛋白質時，尿素(Urea)是肝臟(liver)產生的廢物。健康人的血液(Blood)通常含有6-20 mg / dl（1.8-7.1 mmol / L）的尿素氮(urea nitrogen)。 BUN(Blood Urea Nitrogen)可用作腎臟（腎臟）健康的指標。

Storage	Store all reagents at 4 °C for up to 6 months.
Validity	The validity for this kit is 6 months.
Sample Type	Serum, plasma and other biological fluids.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

1. Centrifuge serum or plasma samples at approximately 1000 × g for 20 mins. If precipitate appears, centrifuge again. Aspirate the supernatant for analysis.

2. Add 6 ml of distilled water to the dye reagent and mix well.

3. Add 1 ml of distilled water to the standard and mix well. Then add 0.01 ml of the diluted standard to 0.99 ml of distilled water and mix well. The final concentration of the standard is 1 mMol/L.

4. Add the following volumes of reagent to relevant wells in a 96-well microplate.

5. Once the solutions are thoroughly mixed, place the tubes in a convection oven at 90°C for 20 minutes

6. Allow microplate to cool, and then read the absorbance of each tube at 520 nm using a spectrophotometer.

乾浴加熱水浴槽 |  Finnpipette F3 微量分注器 |  試管振盪器 Vortex Mixer  |  Genereach迷你型微量離心機  |   ELISA儀器

商品需用儀器測量  酵素免疫分析儀請按此 (標配405、450、492、630 nm, 可擴增至7個濾片)