【聚凝胺(Polybrene; Hexadimethrine bromide)】CAS#28728-55-4 MCE貨號HY-112735
MCE (MedChem Express) -Polybrene
(Synonyms:Hexadimethrine bromide ; 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide)
MCE (MedChemexpress) 總部位於美國新澤西，提供種類齊全的化學及生化研究用產品，包括創新的生命科學試劑，reference compounds (標準化合物)，APIs (原料藥) 及Natural compounds (天然化合物)以提供實驗室或科學研究之用，MedChemExpress(MCE)產品50000+ 特異性抑制劑、化合物庫、重組蛋白，專注於信號通路和疾病研究領域。全球文獻廣泛引用，質控體系嚴格，品質100%。官網連結 https://www.medchemexpress.com
Hexadimethrine bromide Chemical Structure
CAS No. : 28728-55-4
Polybrene | CAS#28728-55-4 MCE貨號HY-112735
聚凝胺(Polybrene; Hexadimethrine bromide)，或稱為hexadimethrine bromide。一種陽離子聚合物(cationic polymer)，常用於哺乳動物細胞之DNA感染實驗，可增強脂質體的感染效率。廣泛用於反轉錄病毒介導((retrovirus vector-mediated))的基因感染，慢病毒介導的基因感染，作用機制可能是通過中和細胞表面唾液酸與病毒顆粒之間的靜電排斥((neutralizing the charge repulsion))從而促進吸附作用。其也是一種有名的抗肝素劑（肝素拮抗劑），常用來生產非特異性凝集的紅細胞。另外也多用於蛋白測序(protein sequencing)，因為小劑量的聚凝胺在自動測序分析可明顯改善多胜肽的降解現象。
聚凝胺(Polybrene; Hexadimethrine bromide)在慢病毒轉導過程中抑制人間充質幹細胞( mesenchymal stem cell )增殖(cell proliferation)。 聚凝胺(Polybrene; Hexadimethrine bromide)在低濃度下被認為是無毒的，但已發現在濃度大於 10 µg/mL 時會對某些細胞類型的細胞增殖產生負面影響。在顯微鏡下觀察時，暴露於聚凝胺(Polybrene; Hexadimethrine bromide)的胰蛋白酶化細胞(Trypsinized cells )明顯變大 。
建議用量為8 µg/ml polybrene (視不同試驗需調整濃度)
| Description | |
|---|---|
| In Vitro |
Hexadimethrine bromide inhibits human mesenchymal stem cell proliferation during lentiviral transduction. Hexadimethrine bromide is considered non-toxic at low concentrations, but has been found to negatively affect cell proliferation in some cell types at concentrations greater than 10 µg/mL. Trypsinized cells exposed to Hexadimethrine bromide are visibly larger in size when viewed under the microscope[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
| Appearance |
Solid |
| Formula |
(C13H30N2)n.Br2 |
| CAS No. | |
| SMILES |
C[N+](CCCCCCC)(C)CCC[N+](C)(C)C.[Br-].[Br-].[n] |
| Shipping |
Room temperature in continental US; may vary elsewhere. |
| Storage |
4°C, stored under nitrogen, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture) |
15 Publications Citing Use of MCE Hexadimethrine bromide
- •Adv Funct Mater. 2019, 1808556.
- •Adv Sci (Weinh). 2022 Jun 2;e2104823. [Abstract]
- •Proc Natl Acad Sci U S A. 2023 Jan 31;120(5):e2208960120. [Abstract]
- •Front Oncol. 2022 Jun 13;12:817660. [Abstract]
- •Biomed Pharmacother. 2020 Jan;121:109578. [Abstract]
- •Front Pharmacol. 2021 Mar 8;12:603453. [Abstract]
- •J Transl Med. 2021 Mar 23;19(1):123. [Abstract]
- •Cell Signal. 2019 Mar 5;58:119-130. [Abstract]
- •Life Sci. 2022 Jan 19;293:120320. [Abstract]
- •J Neuroimmunol. 2023 Apr 15;377:578065. [Abstract]
- •J Gastrointest Oncol. 2022 Jun;13(3):1255-1265. [Abstract]
- •Oncol Lett. 2023 Mar 8.
