三碘甲狀腺原氨酸檢測試劑盒 | EliKine™ Triiodothyronine (T3) ELISA Kit貨號KET0006

三碘甲狀腺原氨酸（Triiodothyronine ,T3）是診斷甲狀腺功能減退(hypothyroidism)和甲狀腺功能亢進(hyperthyroidism)的有用標誌物。甲狀腺功能減退患者的 T3 水平降低，而甲亢患者的 T3 水平升高。 T3 的產生由垂體前葉釋放的促甲狀腺激素 (thyroid-stimulating hormone , TSH) 激活。該途徑是閉環反饋過程的一部分：血漿中 T3 濃度升高會抑制垂體前葉中 TSH 的產生。隨著這些激素濃度的降低，垂體前葉增加 TSH 的產生，通過這些過程，反饋控制系統(feedback control system)穩定了血液中甲狀腺激素的數量。三碘甲狀腺原氨酸檢測試劑盒, 採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的三碘甲狀腺原氨酸（Triiodothyronine ,T3）。偵測範圍: 0.5 ng/ml-8 ng/ml, 最低靈敏度0.4 ng/ml。可應用於以下樣品來源: Other biological fluids, Plasma, Serum

Product name	EliKine™ Triiodothyronine (T3) ELISA Kit
Reactivity	Bovine, Chicken, Dog, Goat, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Swine
Applications	ELISA
Applications notes	EliKine™ T3 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T3 in the sample. The color development is stopped and the intensity of the color is measured.
Detection method	Colorimetric
SampleType	Other biological fluids, Plasma, Serum
Assay type	Competitive ELISA (quantitative)
Assay duration	Multiple steps standard competitive ELISA assay with a working time of 3-4 hours. It depends on the experience of the operation person.
Kit components	• T3 microplate • T3 standard • Biotin conjugated T3 • Avidin-HRP • HRP substrate A • HRP substrate B • Stop solution • Wash buffer • Plate covers.
Features & Benefits	EliKine™ T3 ELISA Kit has high sensitivity and excellent specificity for detection of T3. No significant cross-reactivity or interference between T3 and analogues was observed.
Calibration range	0.5 ng/ml-8 ng/ml
Limit of detection	0.4 ng/ml

TSH甲狀腺促進素檢測 | EliKine™ Human TSH ELISA Kit貨號KET6905

TSH促甲狀腺激素（Thyroid-stimulating hormone） 也稱為促甲狀腺激素(thyrotropin)、促甲狀腺激素(thyrotropic hormone)、TSH 或人類 TSH 的 hTSH）是一種垂體激素(pituitary hormone)，它刺激甲狀腺(thyroid gland)產生甲狀腺素 (thyroxine , T4)，然後是三碘甲狀腺原氨酸 (triiodothyronine, T3)，它刺激幾乎所有的新陳代謝體內的組織。它是由垂體前葉的促甲狀腺細胞合成和分泌的一種糖蛋白激素(glycoprotein hormone)，調節甲狀腺的內分泌功能。 1916 年，Bennett M. Allen 和 Philip E. Smith 發現垂體含有促甲狀腺物質。TSH甲狀腺促進素檢測試劑盒, 採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的TSH促甲狀腺激素（Thyroid-stimulating hormone）。偵測範圍: 0.3 mIU/L-12 mIU/L, 最低靈敏度0.1 mIU/L。可應用於以下樣品來源: Other biological fluids, Plasma, Serum

Product name	EliKine™ Human TSH ELISA Kit
Reactivity	Human
Applications	ELISA
Applications notes	EliKine™ Human TSH ELISA Kit employs a two-site sandwich ELISA to quantitate TSH in samples. An antibody specific for TSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for TSH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method	Colorimetric
SampleType	Other biological fluids, Plasma, Serum
Assay type	Sandwich ELISA (quantitative)
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person.
Alternative	Thyrotropin subunit beta; TSH A; TSH alpha; TSH B; TSH beta; TSHA; TSHB; Thyroid stimulating hormone; Thyroid stimulating hormone alpha chain
Kit components	• Human TSH microplate • Human TSH standard • Human TSH detect antibody • HRP substrate A • HRP substrate B • Stop solution • Wash buffer • Plate covers.
Features & Benefits	EliKine™ Human TSH ELISA Kit has high sensitivity and excellent specificity for detection of Human TSH. No significant cross-reactivity or interference between Human TSH and analogues was observed.
Calibration range	0.3 mIU/L-12 mIU/L
Limit of detection	0.1 mIU/L