【人毛囊真皮乳頭細胞 (Primary cell) 】Human Hair Follicle Dermal Papilla cells貨號T4059

人毛囊真皮乳頭細胞 (Primary cell) | Human Hair Follicle Dermal Papilla cells貨號T4059 5×10⁵ cells / 1.0 ml

人毛囊真皮乳頭細胞Multipolar | 來源Hair Follicle | 建議培養密度6,000 – 10,000 cells per cm^2 。細胞培養條件: 使用PriCoat™T25培養瓶 (貨號G299)，表面塗有I型膠原組成的細胞外基質，以最佳化初代和不朽細胞的生長、增殖和擴增。或塗層細胞外基質(Applied Cell Extracellular Matrix) 增強細胞貼附 (貨號G422)。Grow cells in ECM-coated culture vessels with the following conditions. 培養液Prigrow X series (TM4059)。1%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0°C

初代細胞 (Primary Cell) 是指直接從活體組織中分離出的細胞，具有有限的細胞壽命無法無限制地繼代培養；與一般學術上常用的細胞株 (Cell Line)不同，具有有限的細胞壽命無法無限制地繼代培養，但卻具有最接近活體狀態的細胞型態與功能特性。人毛囊真皮乳頭細胞(Human Hair Follicle Dermal Papilla cells, 貨號 T4059)是從嵌入頭皮毛囊細胞外基質的毛乳頭中分離出來的高活性間充質細胞(mesenchymal cells)。真皮乳頭細胞(Dermal Papilla Cells)通過參與毛囊細胞的上皮-間質相互作用(epithelial-mesenchymal interaction)，在控制毛髮生長周期和產生方面發揮重要作用。它們的存活受信號轉導通路如 ERK 和 Akt 的調節。這些細胞可用於開發和評估毛髮生長產品(hair growth products)以及鑑定毛囊(hair follicle)內的細胞群。此外，由於它們具有雄激素受體(androgen receptors)，可用於雄激素阻斷劑 (androgen blocking reagents) 的體外篩選。

Description	Human Hair Follicle Dermal Papilla cells are highly active mesenchymal cells isolated from the hair papilla embedded in extracellular matrix of scalp hair follicles. Dermal Papilla Cells play a significant role in controlling the hair growth cycle and production by involving in the epithelial-mesenchymal interaction of hair follicle cells. Their survival is regulated by signal transduction pathways such as ERK and Akt. These cells can be used for development and evaluation of hair growth products and identifying cell populations within the hair follicle. In addition, they can be used for in vitro screening of androgen blocking reagents since they have androgen receptors.
SKU	T4059
Species	Human (H. sapiens)
Tissue/Organ/Organ System	Hair Follicle
Cell Morphology	Multipolar
Applications	For Research Use Only
Seeding Density	6,000 – 10,000 cells per cm^2
Population Doubling Time	10 PD. Doubling time: 22.35 hours
Donor Gender	Female
Donor Gender	Female
Donor Age	56
Donor Disease	Normal
Donor Ethnicity	Caucasian
Cell Type	Primary Cells
Growth Properties	Adherent
Propagation Requirements	Use of PriCoat^TMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below. The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM4059. Carbon dioxide (CO₂): 5%, Temperature: 37.0
Subculture Protocol	0
Unit quantity	5×10⁵ cells / 1.0 ml