{"id":9949,"date":"2017-05-08T11:12:34","date_gmt":"2017-05-08T03:12:34","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9949"},"modified":"2025-11-19T10:12:29","modified_gmt":"2025-11-19T02:12:29","slug":"%e4%b8%80%e6%b0%a7%e5%8c%96%e6%b0%ae%e5%90%88%e9%85%b6%ef%bc%88nos%ef%bc%89%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-nitric-oxide-synthase-nos-activity-assay%e8%b2%a8","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=9949","title":{"rendered":"\u4e00\u6c27\u5316\u6c2e\u5408\u9176\uff08NOS\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | Nitric Oxide Synthase (NOS) Activity Assay\u8ca8\u865fK205 (ABCAM, ab211084 Dry Ice)"},"content":{"rendered":"

Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric) \u4e00\u6c27\u5316\u6c2e\u5408\u9176\uff08NOS\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08<\/strong>\u5448<\/strong>\u8272\u6cd5\uff09<\/strong>| <\/strong>\u8ca8\u865f<\/strong>K205 (ABCAM, ab211084 Dry Ice)<\/a><\/span><\/p>\n

Ultrasensitive, Simple, HTP adaptable colorimetric assay kit for NOS activity measurement<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u4e00\u6c27\u5316\u6c2e\u5408\u9176\uff08EC 1.14.13.39\uff09\uff08NOS\uff09\u662f\u50ac\u5316L-\u7cbe\u6c28\u9178\u751f\u7522\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u7684\u4e00\u985e\u9176\u3002\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u5728\u795e\u7d93\u50b3\u905e\uff0c\u8840\u7ba1\u8abf\u7bc0\uff0c\u514d\u75ab\u61c9\u7b54\u548c\u51cb\u4ea1\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002\u5728NADPH\uff0cFAD\uff0cFMN\uff0c\uff086R\uff09-5,6,7,8-\u56db\u6c2b\u751f\u7269\u558b\u5464\uff0c\u9223\u8abf\u86cb\u767d\u548c\u8840\u7d05\u7d20\u7684\u5b58\u5728\u4e0b\uff0cNOS\u7528\u5206\u5b50\u6c27\u50ac\u5316L-\u7cbe\u6c28\u9178\u7684\u80cd\u57fa\u6c2e\u7684\u4e94\u96fb\u5b50\u6c27\u5316\uff0c\u7522\u751fNO\u548cL\u74dc\u6c28\u9178\u3002 NOS\u6709\u4e09\u7a2e\u540c\u7a2e\u578b\uff1a\u5167\u76ae\uff08eNOS\uff09\uff0c\u795e\u7d93\u5143\uff08nNOS\uff09\u548c\u8a98\u5c0e\u578b\uff08iNOS\uff09\u3002 nNOS\u4f54\u4e2d\u6a1e\u795e\u7d93\u7cfb\u7d71NO\u7684\u7522\u751f\uff0c\u5176\u4e2dNO\u53c3\u8207\u7d30\u80de\u901a\u4fe1\u548c\u4fe1\u606f\u5b58\u5132\u3002 eNOS\u5728\u8840\u7ba1\u4e2d\u7522\u751fNO\uff0c\u4e26\u53c3\u8207\u8840\u7ba1\u529f\u80fd\u7684\u8abf\u7bc0\u3002\u8207\u5176\u4ed6\u7570\u69cb\u9ad4\u76f8\u53cd\uff0ciNOS\u5728\u6c27\u5316\u61c9\u6fc0\u689d\u4ef6\u4e0b\u5f9e\u982d\u986f\u793a\uff0c\u4e26\u7522\u751f\u5927\u91cf\u7684NO\u4f5c\u70ba\u8eab\u9ad4\u9632\u79a6\u6a5f\u5236\u7684\u4e00\u90e8\u5206\u3002 BioVision\u7684\u4e00\u6c27\u5316\u6c2e\u5408\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u6e96\u78ba\u548c\u65b9\u4fbf\u7684\u65b9\u6cd5\u4f86\u6e2c\u5b9a\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684NOS\u6d3b\u6027\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u7531NOS\u7522\u751f\u7684\u4e00\u6c27\u5316\u6c2e\u7d93\u6b77\u4e00\u7cfb\u5217\u53cd\u61c9\u4e26\u8207Griess\u8a66\u52911\u548c2\u53cd\u61c9\u4ee5\u7522\u751f\u5728540nm\u5177\u6709\u5f37\u5438\u5149\u5ea6\u7684\u8457\u8272\u7522\u7269\u3002\u8a72\u6e2c\u5b9a\u662f\u7c21\u55ae\uff0c\u654f\u611f\u548c\u9ad8\u901a\u91cf\u9069\u61c9\u6027\uff0c\u53ef\u4ee5\u6aa2\u6e2c\u4f4e\u81f35\u03bcU\u7684NOS\u6d3b\u6027\u3002(ABCAM, ab211084 Dry Ice)<\/p>\n

\"Functional<\/p>\n

\"Functional<\/p>\n

\"Functional<\/p>\n\n\n\n\n\n\n\n\n\n\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
SKU+Size<\/strong><\/td>\nK205-100 (ABCAM, ab211084 Dry Ice)<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nColorimetry (Abs: 540 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian samples<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetection of NOS activity<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, HTP adaptable protocol
\nSensitivity as low as 5 \u00b5U of NOS activity
\nColorimetric detection<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 NOS Assay Buffer
\n\u2022 NOS Dilution Buffer
\n\u2022 NOS Substrate
\n\u2022 NOS Cofactor 1
\n\u2022 NOS Cofactor 2 (25X)
\n\u2022 Nitrate Reductase
\n\u2022 NOS (Positive Control)
\n\u2022 Enhancer
\n\u2022 Nitrite Standard
\n\u2022 Griess Reagent 1
\n\u2022 Griess Reagent 2<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-80\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0Catalog #<\/a><\/td>\nInorganic Ions<\/a>\u76f8\u95dc\u5546\u54c1<\/strong><\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a0K370-100<\/td>\n\u00a0Ammonia Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K470-100<\/td>\n\u00a0Ammonia Colorimetric Assay Kit II<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K380-250<\/td>\n\u00a0Calcium Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0250 assays<\/td>\n<\/tr>\n
\u00a0K530-100<\/td>\n\u00a0Chloride Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K505-100<\/td>\n\u00a0Cobalt Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K390-100<\/td>\n\u00a0Iron Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K385-100<\/td>\n\u00a0Magnesium Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K510-100<\/td>\n\u00a0Nickel Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K262-200<\/td>\n\u00a0Nitric Oxide Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K252-200<\/td>\n\u00a0Nitric Oxide Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K420-100<\/td>\n\u00a0Phosphate Assay Kit (Fluorometric)<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K410-500<\/td>\n\u00a0Phosphate Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n
\u00a0K419-100<\/td>\n\u00a0PicoProbe\u2122 Phosphate Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K387-100<\/td>\n\u00a0Zinc Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorim […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[84,5380],"tags":[3738],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9949"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9949"}],"version-history":[{"count":6,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9949\/revisions"}],"predecessor-version":[{"id":53903,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9949\/revisions\/53903"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9949"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9949"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9949"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}