{"id":9935,"date":"2017-05-04T10:50:47","date_gmt":"2017-05-04T02:50:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9935"},"modified":"2022-07-22T10:32:27","modified_gmt":"2022-07-22T02:32:27","slug":"9935","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=9935","title":{"rendered":"PARP\u9ad4\u5167\u85e5\u6548\u5b78\u8a66\u9a57II\u8a66\u5291\u76d2 | PARP in vivo Pharmacodynamic Assay II Kit \u8ca8\u865f4520-096-K"},"content":{"rendered":"<p><strong>PARP<\/strong><strong>\u9ad4\u5167\u85e5\u6548\u5b78\u8a66\u9a57II\u8a66\u5291\u76d2 | 4520-096-K<\/strong><\/p>\n<p><strong>\u7522\u54c1\u4ecb\u7d39<\/strong><strong>:<\/strong> \u805a\uff08ADP-\u6838\u7cd6\uff09\u805a\u5408\u9176\uff08PARP\uff09\u662f\u76f8\u95dc\u9176\u7684\u5bb6\u65cf\uff0c\u5176\u50ac\u5316ADP-\u6838\u7cd6\u8f49\u79fb\u5230\u9776\u86cb\u767d\u4e0a\u3002\u901a\u904e\u805a\uff08ADP-\u6838\u7cd6\uff09\u7cd6\u6c34\u89e3\u9176\uff08PARG\uff09\u7684PAR\u5408\u6210\u548c\u96a8\u5f8c\u7684\u964d\u89e3DNA\u4fee\u5fa9\u4e2d\u626e\u6f14\u91cd\u8981\u4f5c\u7528\u3002\u67d0\u4e9b\u816b\u7624\u5728\u540c\u6e90\u91cd\u7d44\u4fee\u5fa9\u4e2d\u6709\u7f3a\u9677\u4e26\u4e14\u4f9d\u8cf4\u65bcPARP\u4ecb\u5c0e\u7684DNA\u4fee\u5fa9\uff0c\u7528\u65bc\u5b58\u6d3b\u7684\u767c\u73fePARP\u548cPARG\u4f5c\u70ba\u764c\u75c7\u6cbb\u7642\u4e2d\u7684\u6f5b\u5728\u9776\u6a19\u3002\u53e6\u5916\uff0c\u7aef\u7c92\u91761\uff08PARP 5a\uff09\u5728\u7aef\u7c92\u4f38\u9577\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002\u6f5b\u5728\u7684PARP\uff0cPARG\u548c\u963b\u805a\u9176\u6291\u5236\u5291\u7684\u7be9\u9078\u5de5\u5177\u5c0d\u85e5\u7269\u767c\u73fe\u5f88\u91cd\u8981\u3002Trevigen\u63d0\u4f9b\u8a66\u5291\u76d2\uff0c\u5305\u62ec\u7d14\u5316\u9176\uff0c\u6297\u9ad4\u548c\u8a66\u5291\uff0c\u7528\u65bc\u6e2c\u91cfPARP 1\uff0c\u963b\u805a\u91761\uff0cPARG\u548cPAR\u3002\u9019\u4e9b\u6e2c\u5b9a\u975e\u5e38\u9069\u5408\u65bc\u5316\u5408\u7269\u5927\u898f\u6a21\u7be9\u9078\uff0c\u6f5b\u5728\u7684PARP\u6291\u5236\u5291\u7684IC 50\u6e2c\u5b9a\u4ee5\u53ca\u7d30\u80de\u63d0\u53d6\u7269\u6216\u5916\u5468\u8840\u55ae\u6838\u7d30\u80de\u4e2d\u9ad4\u5167\u6d3b\u6027\u7684\u6e2c\u5b9a\u3002\u6b64\u5916\uff0cPARP 1\u548cPAR\u7684\u55ae\u514b\u9686\u548c\u591a\u514b\u9686\u6297\u9ad4\u53ef\u7528\uff0c\u5305\u62ec\u7528\u65bc\u6aa2\u6e2c\u7684\u751f\u7269\u7d20\u5316NAD +\u3002<\/p>\n<p><strong>\u7522\u54c1\u7279\u9ede<\/strong><strong>:\u00a0<\/strong>HT PARP\u9ad4\u5167\u85e5\u6548\u5b78\u6e2c\u5b9aII \u5206\u6790\u5957\u7d44\uff0c\u57fa\u65bcPAR\u6c34\u5e73\u76e3\u6e2c\u7d30\u80de\u5167PARP\u6d3b\u6027\u3002\u572896\u5b54\u677f\u7684\u5b54\u4e2d\u56fa\u5b9a\u7684PAR\u55ae\u514b\u9686\u6297\u9ad4\u6355\u7372\u9644\u8457\u65bc\u86cb\u767d\u8cea\u7684\u7d30\u80dePAR\u548cPAR\uff1b\u7528\u591a\u514b\u9686PAR\u6aa2\u6e2c\u6297\u9ad4\u5b75\u80b2\uff0c\u7136\u5f8c\u52a0\u5165\u5c71\u7f8a\u6297\u5154IgG-HRP\u4e8c\u7d1a\u5316\u5408\u7269\u548c\u5316\u5b78\u767c\u5149HRP\u5e95\u7269\uff0c\u7522\u751f\u8207\u7d30\u80dePAR\u76f4\u63a5\u76f8\u95dc\u7684\u76f8\u5c0d\u5149\u55ae\u4f4d\uff08RLU\uff09\u3002\u9810\u5305\u88ab\u768496\u5b54\u6355\u7372\u6297\u9ad4\u677f\u3001\u4fe1\u566a\u6bd4\u9ad8\u3001\u6aa2\u6e2c\u9748\u654f\u5ea6\u70ba2 pg \/ ml\u7684PAR \u5bec\u7dda\u6027\u52d5\u614b\u7bc4\u570d\u70ba1,000 pg \/ ml\u3001\u6e1b\u5c11\u6e2c\u5b9a\u9593\u8b8a\u7570\u6027\u3002<strong>\u7522\u54c1<\/strong><strong>\u61c9\u7528\uff1a<\/strong>\u5b9a\u91cf\u5916\u5468\u8840\u55ae\u6838\u7d30\u80de\uff0c\u7d44\u7e54\u57f9\u990a\u7d30\u80de\u548c\u4f86\u81ea\u4e0d\u540c\u7d44\u7e54\uff0c\u5668\u5b98\u548c\u7570\u7a2e\u79fb\u690d\u7269\u7684\u816b\u7624\u88c2\u89e3\u7269\u4e2d\u7684PAR\u542b\u91cf\u3002\u76e3\u6e2cPARP\u6291\u5236\u5291\u5c0d\u9ad4\u5167PAR\u5f62\u6210\u7684\u529f\u6548\u3002\u9a57\u8b49\u7531PARP\u6291\u5236\u5291\/\u6297\u764c\u85e5\u7269\u806f\u5408\u6cbb\u7642\u5f15\u8d77\u7684\u589e\u5f37\u7684\u764c\u7d30\u80de\u7d30\u80de\u6bd2\u6027\u4e4b\u89c0\u5bdf\u3002\u4fc3\u9032PARP\u548cPARG\u9776\u5411\u6cbb\u7642\u7684\u767c\u5c55\u3002<\/p>\n<table style=\"height: 403px;\" width=\"449\">\n<tbody>\n<tr>\n<td colspan=\"3\" width=\"807\"><strong>PARP<\/strong><strong>\u9ad4\u5167\u85e5\u6548\u5b78\u8a66\u9a57II\u8a66\u5291\u76d2<\/strong><strong>\u5167\u5bb9\u7269<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"212\">Catalog Number<\/td>\n<td width=\"521\">Description<\/td>\n<td width=\"70\">Qty<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-01<\/td>\n<td width=\"521\">PAR Standard, 25 pg\/\u00b5l<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-08<\/td>\n<td width=\"521\">Jurkat Cell Lysate Control Standard, Low<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-09<\/td>\n<td width=\"521\">Jurkat Cell Lysate Control Standard, Medium<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-10<\/td>\n<td width=\"521\">Jurkat Cell Lysate Control Standard, High<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-03<\/td>\n<td width=\"521\">PAR Polyclonal Detecting Antibody<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-04<\/td>\n<td width=\"521\">Goat anti-Rabbit IgG-HRP<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-06<\/td>\n<td width=\"521\">DNase I, 2 Units\/\u00b5l<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-02<\/td>\n<td width=\"521\">Sample Buffer<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-05<\/td>\n<td width=\"521\">Cell Lysis Reagent<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-07<\/td>\n<td width=\"521\">100X Magnesium Cation<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-11<\/td>\n<td width=\"521\">Antibody Diluent<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-12<\/td>\n<td width=\"521\">20% SDS<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4520-096-P<\/td>\n<td width=\"521\">Pre-coated white 96-stripwell plate and 5 sealers<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4675-096-01<\/td>\n<td width=\"521\">PARP PeroxyGlow\u2122 A<\/td>\n<td>1<\/td>\n<\/tr>\n<tr>\n<td width=\"212\">4675-096-02<\/td>\n<td width=\"521\">PARP PeroxyGlow\u2122 B<\/td>\n<td>\u00a01<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><span style=\"color: #ff0000;\">\u76f8\u95dc\u7522\u54c1<\/span> <span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=9870\">\u5f57\u661f\u5206\u6790\u5957\u7d44<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=8958\">\u5f57\u661f\u5206\u6790\u5c08\u7528\u96fb\u6cf3\u8a2d\u5099<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=9841\">hOGG1 DNA\u4fee\u5fa9\u9176<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7234\">\u7e3d\u6297\u6c27\u5316\u80fd\u529b\u5206\u6790<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6196\">DNA\u96fb\u6cf3\u69fd<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7987\">\u9280\u67d3\u8a66\u5291\u7d44<\/a><\/span><\/strong><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PARP\u9ad4\u5167\u85e5\u6548\u5b78\u8a66\u9a57II\u8a66\u5291\u76d2 | 4520-096-K \u7522\u54c1\u4ecb\u7d39: \u805a\uff08ADP-\u6838\u7cd6\uff09\u805a\u5408\u9176\uff08PARP\uff09\u662f [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":9764,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[121,183,498],"tags":[3740],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9935"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9935"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9935\/revisions"}],"predecessor-version":[{"id":9940,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9935\/revisions\/9940"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/9764"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9935"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9935"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9935"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}