{"id":9813,"date":"2017-04-28T21:21:32","date_gmt":"2017-04-28T13:21:32","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9813"},"modified":"2023-08-17T12:36:36","modified_gmt":"2023-08-17T04:36:36","slug":"%e8%9b%8b%e7%99%bd%e7%be%b0%e5%9f%ba%e5%90%ab%e9%87%8f%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bd%9cprotein-carbonyls-assay%e8%b2%a8%e8%99%9fk810-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=9813","title":{"rendered":"\u86cb\u767d\u7fb0\u57fa\u542b\u91cf\u5448\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\uff5cProtein carbonyls Assay\u8ca8\u865fK830-100 (ABCAM ab126287)"},"content":{"rendered":"

\u5546\u54c1<\/strong>: \u86cb\u767d\u7fb0\u57fa\u542b\u91cf\u6e2c\u5b9a\u8a66\u5291\u76d2\uff5c\u8ca8\u865fK830-100 (ABCAM ab126287)<\/a><\/span><\/strong><\/p>\n

Simple assay to quantify protein carbonyls<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Protein carbonyl groups\u86cb\u767d\u8cea\u7fb0\u57fa\u662foxidative stress\u6c27\u5316\u61c9\u6fc0\u7684\u91cd\u8981\u4e14\u76f4\u63a5\u7684\u751f\u7269\u6a19\u8a8c\u7269\u3002 \u86cb\u767d\u7fb0\u57fa\u5316\u7684DNPH\u6a19\u8a18\u4e00\u76f4\u662f\u6c27\u5316\u61c9\u6fc0\u6700\u5e38\u898b\u7684\u63aa\u65bd\u4e4b\u4e00\u3002 \u7531\u53cd\u61c9\u5f62\u6210\u7684DNP hydrazones\u53ef\u4ee5\u5728375nm\u6e2c\u91cf\u3002 BioVision\u7684\u86cb\u767d\u7fb0\u57fa\u542b\u91cf\u6e2c\u5b9a\u8a66\u5291\u76d2\u65e8\u5728\u63d0\u4f9b\u4e00\u7a2e\u7c21\u55ae\u800c\u6e96\u78ba\u7684\u86cb\u767d\u8cea\u6a23\u54c1\u4e2d\u7fb0\u57fa\u91cf\u5316\u65b9\u6cd5\u3002 \u4f7f\u7528BSA\u4f5c\u70ba\u5be6\u4f8b\uff0c1mg\uff08\u301c15nmol\uff09\u6a23\u54c1\u5177\u6709\u7d040.15nmol\u7fb0\u57fa\u7684\u6aa2\u6e2c\u9650\uff0c\u5176\u4e2dBSA\u901a\u5e38\u542b\u6709\u7d041-3nmol\u7684\u7fb0\u57fa\/ mg<\/p>\n

Protein carbonyl groups are an important and immediate biomarker of oxidative stress. DNPH tagging of protein carbonyls has been one of the most common measures of oxidative stress. DNP hydrazones formed from the reaction are easily quantifiable at 375 nm. BioVision\u2019s Protein Carbonyl Content Assay Kit is designed to provide a simple and accurate method of quantifying carbonyls in protein samples. Using BSA as an example, a 1 mg (~ 15 nmol) sample has a detection limit of about 0.15 nmol carbonyl, where BSA typically contains approximately 1-3 nmol carbonyl\/mg.<\/p>\n

\"\"\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n
Size<\/td>\n100 assays<\/td>\n<\/tr>\n
SKU+Size<\/td>\nK830-100 (ABCAM ab126287)<\/td>\n<\/tr>\n
Detection Method<\/td>\nAbsorbance ( 375 and 562 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/td>\nN\/A<\/td>\n<\/tr>\n
Features & Benefits<\/td>\n\u2022 Simple procedure; takes ~ 30-90 minutes<\/td>\n<\/tr>\n
Kit Components<\/td>\n\u2022 DNPH Solution\u2022 100% TCA Solution\u2022 10% Streptozocin Solution\u2022 6 M Guanidine Solution\u2022 96-Well Clear Plate<\/td>\n<\/tr>\n
Storage Conditions<\/td>\n4\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

\n
Somanah, J. et al., (2017) Extracts of Mauritian Carica papaya (var solo) protect SW872 and HepG2 cells against hydrogen peroxide induced oxidative stress,\u00a0Journal of Food Science and Technology, Apr.2017, 54(7), 1917-1927<\/a><\/div>\n<\/div>\n
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Gaspard & McMaster. The Mitochondrial Quality Control Protein Yme1 Is Necessary to Prevent Defective Mitophagy in a Yeast Model of Barth Syndrome.\u00a0J. Biol. Chem.,\u00a0Apr 2015; 290: 9284 – 9298.<\/a><\/div>\n<\/div>\n
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Wilson et al., GCN2 is required to increase fibroblast growth factor 21 and maintain hepatic triglyceride homeostasis during asparaginase treatment.Am J Physiol Endocrinol Metab,\u00a0Feb 2015; 308: E283 – E293.<\/a><\/div>\n<\/div>\n

\u5176\u5b83\u5546\u54c1<\/strong>: <\/strong>\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790\u5957\u7d44<\/a> | CETP\u81bd\u56fa\u9187\u916f\u8f49\u904b\u86cb\u767dELISA<\/a> | Irisin\u7af6\u722d\u578bELISA<\/a> | Free Fatty Acid\u5448\u8272\u87a2\u5149\u5206\u6790<\/a> | Triglyceride\u4e09\u9178\u7518\u6cb9\u916f\u5b9a\u91cf\u5206\u6790<\/a> \u3010\u67d3\u8272\u5546\u54c1\u3011<\/strong> Lipid\u8102\u8cea\u67d3\u8272\u5957\u7d44<\/a> | \u81bd\u56fa\u9187\u67d3\u8272\u5957\u7d44<\/a> \u3010\u8403\u53d6\u5546\u54c1\u3011<\/strong>\u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6<\/a><\/p>\n

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 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u5546\u54c1: \u86cb\u767d\u7fb0\u57fa\u542b\u91cf\u6e2c\u5b9a\u8a66\u5291\u76d2\uff5c\u8ca8\u865fK830-100 (ABCAM ab126287) Simple assa […]<\/p>\n","protected":false},"author":3,"featured_media":6786,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[467,122],"tags":[3763],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9813"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9813"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9813\/revisions"}],"predecessor-version":[{"id":16659,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9813\/revisions\/16659"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6786"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9813"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9813"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9813"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}