{"id":9443,"date":"2017-04-13T13:23:14","date_gmt":"2017-04-13T05:23:14","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9443"},"modified":"2023-02-04T17:57:11","modified_gmt":"2023-02-04T09:57:11","slug":"mitochondria%e7%b2%92%e7%b7%9a%e9%ab%94%e5%88%86%e9%9b%a2%e5%a5%97%e7%b5%84-mitochondria-isolation-kit%e8%b2%a8%e8%99%9fk288-50","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=9443","title":{"rendered":"Mitochondria\u7c92\u7dda\u9ad4\u5206\u96e2\u5957\u7d44 | Mitochondria Isolation Kit\u8ca8\u865fK288-50 (ABCAM ab288084)"},"content":{"rendered":"

Mitochondria<\/em><\/strong>\u7c92\u7dda\u9ad4\u5206\u96e2\u5957\u7d44 <\/strong>| \u8ca8\u865f<\/strong>K288-50<\/a>\u00a0\u00a0(ABCAM ab288084)\u00a0<\/span><\/p>\n

Mammalian Mitochondria Isolation Kit For Tissue & Cultured Cells<\/em><\/strong><\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\" \u7dda\u7c92\u9ad4\u662f\u7d30\u80de\u7684\u52d5\u529b\uff0c\u4ee5\u4e09\u78f7\u9178\u817a\u82f7\uff08ATP\uff09\u7684\u5f62\u5f0f\u7522\u751f\u5927\u90e8\u5206\u80fd\u91cf\u4f9b\u61c9\u3002 \u7dda\u7c92\u9ad4\u662f\u96d9\u819c\u7d30\u80de\u5668\uff1a\u5916\u819couter membrane\u548cfolded inner membrane\u00a0\u6298\u758a\u5167\u819c\uff0c\u7a31\u70ba\u5d74 (cristae)\u3002\u5206\u96e2\u7684\u7dda\u7c92\u9ad4\u662f\u7814\u7a76\u7dda\u7c92\u9ad4\u547c\u5438(mitochondrial respiration)\uff0c\u547c\u5438\u8907\u5408\u7269\u7d44\u88dd(assembly of the respiratory complexes)\uff0c\u7d30\u80de\u51cb\u4ea1(\u00a0apoptosis)\uff0cmtDNA\u548cmtRNA\u4ee5\u53ca\u7dda\u7c92\u9ad4\u86cb\u767d\u8cea\u8b5c (mitochondrial protein profiling)\u5206\u6790\u7684\u9996\u9078\u6a23\u54c1\u3002Mitochondria<\/em><\/strong>\u7c92\u7dda\u9ad4\u5206\u96e2\u5957\u7d44<\/strong><\/a>\u70ba\u5b8c\u6574\u7dda\u7c92\u9ad4\u7684\u5206\u96e2\u63d0\u4f9b\u4e86\u5169\u500b\u9078\u64c7\u3002\u7b2c\u4e00\u500b\u9078\u64c7\u662f\u5229\u7528\u57fa\u65bc\u8a66\u5291\u7684\u65b9\u6cd5\uff0c\u5141\u8a31\u4e26\u884c\u8655\u7406\u591a\u500b\u6a23\u54c1\u3002 \u7b2c\u4e8c\u500b\u9078\u64c7\u4f7f\u7528\u50b3\u7d71\u7684\u5747\u52fb\u5206\u88c2\uff0c\u5176\u63d0\u4f9b\u66f4\u597d\u7684\u7dda\u7c92\u9ad4\u7522\u91cf\u3002<\/p>\n

<\/div>\n

\"\"<\/p>\n

\u7c92\u817a\u9ad4\u81ea\u7d30\u80de\u6216\u7d44\u7e54\u5206\u96e2\u5f8c, \u76ee\u524d\u662f\u4ee5JC-1\u67d3\u5291\u6e2c\u91cf\u3002\u5206\u96e2\u5f8c\u4e4b\u7c92\u817a\u9ad4\u901a\u904e\u4f7f\u7528JC-1\u67d3\u6599\uff0c\u5206\u6790\u7d14\u5316\u7684\u7dda\u7c92\u9ad4\u7684\u5b8c\u6574\u6027\uff0c\u6e2c\u8a66\u96fb\u5316\u5b78\u8cea\u5b50\u68af\u5ea6\uff08\u0394\u03a8\uff09\u7684\u5167\u7dda\u7c92\u9ad4\u819c\u3002 \u6e2c\u91cf\u65b9\u5f0f\u70ba: \u7d14\u5316\u7684\u7dda\u7c92\u9ad4\u5728\u51b0\u4e0a\u89e3\u51cd\uff0c\u4e26\u8207JC-1\u67d3\u6599\uff081\u03bcg\/ ml\uff09\u572837\u2103\u4e00\u8d77\u5b75\u80b215\u5206\u9418\u3002 \u5b8c\u6574\u7684\u7d14\u5316\u7dda\u7c92\u9ad4\u986f\u793aJC-1\u67d3\u6599\u7684\u805a\u96c6\uff0c\u5176\u4fe1\u865f\u53ef\u4ee5\u5728Ex \/ Em = 530 \/ 590nm\u6e2c\u91cf\u3002\u7528\u6297\u9ef4\u7d20A\uff08100\u03bcM\uff09\u8655\u7406\u6d88\u9664\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\uff0c\u5c0e\u81f4\u87a2\u5149\u4fe1\u865f\u964d\u4f4e\u3002<\/p>\n

\u7522\u54c1\u4f7f\u7528\u6700\u4f73\u5316:<\/strong> \u70ba\u4e86\u907f\u514d\u86cb\u767d\u8cea\u964d\u89e3\uff0c\u5efa\u8b70\u5728\u7dda\u7c92\u9ad4\u4e2d\u6dfb\u52a0\u86cb\u767d\u9176\u6291\u5236\u5291\u6df7\u5408\u7269\uff08Cat\uff03K272-1EA\uff09\u3002 \u7d30\u80de\u88c2\u89e3\u6548\u7387\u6aa2\u67e5\uff0c\u53ef\u5c075\u03bcl\u7d30\u80de\u88c2\u89e3\u6db2\u653e\u5165\u8f09\u73bb\u7247\u4e2d\uff0c\u4e26\u52a0\u84cb\u73bb\u7247\u4e26\u5728\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\u3002\u5c0d\u65bc\u7d44\u7e54\uff0c\u9032\u884c\u8db3\u5920\u7684\u00a0strokes\u4e0b\u5373\u7372\u5f97\u5747\u52fb\u7684\u61f8\u6d6e\u6db2\u7d30\u80de\u3002 \u6216\u4f7f\u7528Dounce Tissue Homogenizer \u7d44\u7e54\u7814\u78e8\u5668<\/strong><\/span>\u5747\u8cea\u8edf\u7d44\u7e54\uff08\u5982\u8178\u9053\u3001\u80ba\u3001\u5fc3\u7b49\u7d44\u7e54\uff09\u4ee5\u53ca\u7d30\u80de\u3002<\/p>\n\n\n\n\n\n\n\n\n
Size<\/strong><\/td>\n50 assays<\/td>\n<\/tr>\n
SKU+Size<\/strong><\/td>\nK288-50<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nUseful for mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and protein profiling.
\nWestern blot and ELISA.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple & rapid method to isolate highly pure, intact and functional mitochondria from tissues and cultured cells.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Mitochondria Isolation Buffer
\n\u2022 Reagent A
\n\u2022 Storage Buffer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

BCA<\/strong>\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/strong>\u00a0<\/strong><\/a>|\u00a0<\/strong>\u8ca8\u865f<\/strong>K812-1000\u00a0 \u86cb\u767d\u8cea\u6fc3\u5ea6\u7bc4\u570d\uff080.01-0.6\u6beb\u514b\/\u6beb\u5347\uff09<\/a>\u00a0<\/span><\/p>\n

BCA<\/strong>\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/strong><\/a>|\u00a0<\/strong>\u8ca8\u865f<\/strong>K813-5000\u00a0 \u86cb\u767d\u8cea\u6fc3\u5ea6\u7bc4\u570d25-2000\u03bcg\/ ml<\/a>\u00a0<\/span><\/p>\n

BCA<\/strong>\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/strong> \u9084\u539f\u5291\u517c\u5bb9<\/strong>\u00a0<\/strong><\/a>|\u00a0<\/strong>(562nm) 25-2000\u03bcg\/ ml \u8ca8\u865f<\/strong>K818-1000<\/a>\u00a0<\/span><\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a>\"102-T\"<\/a>\"0100-01\"<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Mitochondria\u7c92\u7dda\u9ad4\u5206\u96e2\u5957\u7d44 | \u8ca8\u865fK288-50\u00a0\u00a0(ABCAM ab288084)\u00a0 Mamm […]<\/p>\n","protected":false},"author":3,"featured_media":6512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[129,89,1],"tags":[3842],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9443"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9443"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9443\/revisions"}],"predecessor-version":[{"id":37872,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9443\/revisions\/37872"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6512"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9443"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9443"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9443"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}