{"id":9418,"date":"2017-04-13T11:27:25","date_gmt":"2017-04-13T03:27:25","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9418"},"modified":"2023-05-12T11:06:23","modified_gmt":"2023-05-12T03:06:23","slug":"%e7%b7%9a%e7%b2%92%e9%ab%94%e8%a4%87%e5%90%88%e7%89%a9i%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-mitochondrial-complex-i-activity-assay-%e8%b2%a8%e8%99%9fk968-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=9418","title":{"rendered":"\u7dda\u7c92\u9ad4\u8907\u5408\u7269I\u6d3b\u6027\u5206\u6790\u5957\u7d44 | Mitochondrial Complex I Activity Assay \u8ca8\u865fK968-100(ABCAM \u8ca8\u865fab287847)"},"content":{"rendered":"

\u7dda\u7c92\u9ad4\u8907\u5408\u7269<\/strong>I<\/strong>\u6d3b\u6027\u5206\u6790\u5957\u7d44<\/strong> \u00a0| \u8ca8\u865f<\/strong>K968-100(ABCAM \u8ca8\u865fab287847)<\/strong><\/a><\/span><\/p>\n

Mitochondrial Complex I Activity Colorimetric Assay Kit<\/em><\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong><\/span><\/p>\n

\u7dda\u7c92\u9ad4\u8907\u5408\u7269I\u6216NADH\uff1a\u6cdb\u918c\u6c27\u5316\u9084\u539f\u9176NADH:ubiquinone oxidoreductase\uff08EC 1.6.5.3\uff09\u662f\u4f4d\u65bc\u7dda\u7c92\u9ad4\u819c\u4e2d\u7684\u96fb\u5b50\u50b3\u8f38\u93c8\u4e2d\u7b2c\u4e00\u500b\u4e5f\u662f\u6700\u5927\u7684\u8907\u5408\u7269\u3002\u5b83\u5c07NADH\u6c27\u5316\u6210NAD +\uff0c\u4e26\u5c07\u96fb\u5b50\u8f49\u79fb\u5230\u6cdb\u918c\uff08\u4e5f\u5b58\u5728\u65bc\u5167\u7dda\u7c92\u9ad4\u819c\u4e2d\uff09\uff0c\u5c07\u5176\u8f49\u5316\u70ba\u6cdb\u9187ubiquinol\u3002\u5728\u6b64\u904e\u7a0b\u4e2d\uff0c\u5b83\u5c07\u8cea\u5b50\u50b3\u905e\u5230\u5167\u7dda\u7c92\u9ad4\u819c\u4e0a\uff0c\u6709\u52a9\u65bc\u958b\u767c\u96fb\u5316\u5b78\u68af\u5ea6\u3002\u9019\u500b\u904e\u7a0b\u5c0d\u7d30\u80de\u547c\u5438\u975e\u5e38\u91cd\u8981\uff0c\u5c0d\u8907\u96dcI\u6d3b\u6027\u7684\u4e0d\u5229\u5f71\u97ff\u53ef\u80fd\u6703\u640d\u5bb3\u7dda\u7c92\u9ad4\u547c\u5438\uff0c\u9032\u4e00\u6b65\u5c0e\u81f4\u7d30\u80de\u61c9\u6fc0\u3002 BioVision\u7684\u7dda\u7c92\u9ad4\u8907\u5408\u7269I\u6e2c\u5b9a\u8a66\u5291\u76d2\u662f\u78ba\u5b9a\u5206\u96e2\u7684\u7dda\u7c92\u9ad4\u4e2d\u5fa9\u5408\u7269I\u7684\u6d3b\u6027\u7684\u5feb\u901f\u53ef\u9760\u7684\u65b9\u6cd5\u3002\u5b83\u53ef\u7528\u65bc\u5206\u96e2\u7dda\u7c92\u9ad4\u7684\u547c\u5438\u7814\u7a76\uff0c\u53ef\u7528\u65bc\u7814\u7a76\u6bd2\u7269\uff0c\u85e5\u7269\u548c\u5176\u4ed6\u74b0\u5883\u689d\u4ef6\u5c0d\u7dda\u7c92\u9ad4\u8907\u5408\u7269I\u6d3b\u6027\u7684\u5f71\u97ff\u3002\u8a72\u8a66\u5291\u76d2\u4f7f\u7528\u6cdb\u918c\u916e\u985e\u4f3c\u7269\uff0c\u4f5c\u70ba\u96fb\u5b50\u53d7\u9ad4\uff0c\u5176\u901a\u904e\u8907\u5408\u7269I\u7684\u50ac\u5316\u6d3b\u6027\u8f49\u5316\u70ba\u5341\u5206\u591a\u70ef\u9187\u3002\u4ee5600nm\u5438\u6536\u5176\u6c27\u5316\u5f62\u5f0f\u7684\u5149\u7684\u8907\u5408\u7269I\u67d3\u6599\u7528\u4f5c\u63a5\u53d7\u7684\u672b\u7aef\u96fb\u5b50\u53d7\u9ad4\u4f86\u81eadecylubiquinol\u7684\u96fb\u5b50\u3002\u901a\u904e\u8a18\u9304\u9084\u539f\u7684\u8907\u5408\u7269I\u67d3\u6599\u5728600nm\u7684\u5438\u5149\u5ea6\u8b8a\u5316\u901a\u904e\u6bd4\u8272\u6cd5\u6e2c\u5b9a\u8907\u5408\u7269I\u6d3b\u6027\u3002\u901a\u904e\u5f9e\u7e3d\u6d3b\u6027\u4e2d\u6e1b\u53bb\u8907\u5408\u7269I\u6291\u5236\u5291\u9b5a\u85e4\u916e\u7684\u5b58\u5728\u800c\u6e1b\u53bb\u6d3b\u6027\u4f86\u7372\u5f97\u7279\u7570\u6027\u8907\u5408\u7269I\u6d3b\u6027\u3002\u8a72\u8a66\u5291\u76d2\u53ef\u4ee5\u6aa2\u6e2c\u4f4e\u81f30.1 mU \/Well\uff0c\u7dda\u6027\u9ad8\u90547 mU \/Well\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
SKU+Size<\/strong><\/td>\nK968-100 (ab287847)\u00a0<\/strong><\/a><\/span><\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nColorimetric (Absorbance 600 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMultiple species<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of Complex I activity in isolated mitochondria<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, rapid & convenient assay to measure Mitochondrial Complex I
\n\u2022 Includes Inhibitor (Rotenone)<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Complex I Assay Buffer
\n\u2022 NADH \u2022 Decylubiquinone
\n\u2022 Complex I Dye
\n\u2022 Complex I Inhibitor Rotenone
\n\u2022 Clear 96-well half area plate<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7dda\u7c92\u9ad4\u8907\u5408\u7269I\u6d3b\u6027\u5206\u6790\u5957\u7d44 \u00a0| \u8ca8\u865fK968-100(ABCAM \u8ca8\u865fab287847) Mitochond […]<\/p>\n","protected":false},"author":3,"featured_media":8363,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[129],"tags":[3849],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9418"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9418"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9418\/revisions"}],"predecessor-version":[{"id":42370,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9418\/revisions\/42370"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8363"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9418"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9418"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9418"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}